LOCUS       sequence-353844-        4220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_5a7_pKD46.10
FEATURES             Location/Qualifiers
     source          1..4220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             277..1467
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(629..648)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(1594..2544)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2592..2814
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3097..3183)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3347..3381
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3384..3433
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3499..3507
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta
      241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
      301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
      601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg
      781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc
     1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc
     1501 gtacaattct gaggtccaca cagatagtgt cctgttctac ttttgtttgt tagtcttgat
     1561 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     1621 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
     1681 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     1741 taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt
     1801 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     1861 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     1921 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     1981 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     2041 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     2101 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     2161 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     2221 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     2281 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     2341 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga
     2401 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     2461 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     2521 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     2581 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
     2641 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
     2701 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     2761 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     2821 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     2881 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg cggccgcaat
     2941 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt
     3001 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac
     3061 tacaggcgcg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt
     3121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     3181 ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa tctggtgtaa
     3241 cttcggaatc gtccccaatt attgaacacc cttcggggtg tttttttgtt tctggtctac
     3301 catctcgttg tgataataga cctgaagtgc ctactctgga aaatctttga cagctagctc
     3361 agtcctaggt ataatgctag cagctgtcac cggatgtgct ttccggtctg atgagtccgt
     3421 gaggacgaaa cagcctctac aaataatttt gtttaaggct cgctacgaga agattgttac
     3481 tttcgcagcg ttattatcta aggaggtagt ccatggttag caaaggcgag gcggttatca
     3541 aggagtttat gcgttttaag gttcacatgg agggtagcat gaatggtcac gagttcgaga
     3601 tcgagggtga aggcgagggt cgtccgtacg aaggcaccca gaccgcgaag ctgaaagtga
     3661 ccaagggtgg cccgctgccg ttcagctggg acatcctgag cccgcagttc atgtatggca
     3721 gccgtgcgtt taccaaacac ccggcggaca ttccggatta ctataagcaa agcttcccgg
     3781 aaggttttaa atgggagcgt gttatgaact tcgaagatgg tggcgcggtg accgttaccc
     3841 aggacaccag cctggaggat ggcaccctga tttacaaggt gaaactgcgt ggcaccaact
     3901 ttccgccgga tggtccggtt atgcagaaga aaacgatggg ttgggaagcg agcaccgagc
     3961 gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa aatggcgctg cgtctgaagg
     4021 acggtggccg ttacctggcg gattttaaga ccacctataa agcgaagaaa ccggtgcaaa
     4081 tgccgggtgc gtacaacgtt gaccgtaaac tggatattac cagccacaac gaggattata
     4141 ccgtggttga gcaatatgag cgtagcgagg gtcgccacag caccggcggc atggacgaac
     4201 tgtataaggg atcctaataa
//
