LOCUS       sequence-353845-        3602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_49.10
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             398..1189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1288..1876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1777..1796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2030..2047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2062..2202
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2128..2147)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2288..2310
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2304..2495)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2675..2761)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2833..2867
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2876..2887
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg
     1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg
     1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2101 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2161 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2221 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2281 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2341 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2401 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2461 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2521 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2581 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2641 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2701 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2761 gatgccttta attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact
     2821 tcggaatcgt cctttacggc tagctcagtc ctaggtatag tgctagctac tagtgaaaga
     2881 ggagaaatac tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta
     2941 aggttcacat ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg
     3001 gtcgtccgta cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc
     3061 cgttcagctg ggacatcctg agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac
     3121 acccggcgga cattccggat tactataagc aaagcttccc ggaaggtttt aaatgggagc
     3181 gtgttatgaa cttcgaagat ggtggcgcgg tgaccgttac ccaggacacc agcctggagg
     3241 atggcaccct gatttacaag gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg
     3301 ttatgcagaa gaaaacgatg ggttgggaag cgagcaccga gcgtctgtat ccggaagatg
     3361 gcgtgctgaa gggtgatatc aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg
     3421 cggattttaa gaccacctat aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg
     3481 ttgaccgtaa actggatatt accagccaca acgaggatta taccgtggtt gagcaatatg
     3541 agcgtagcga gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat
     3601 aa
//
