LOCUS       sequence-353846-        3873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_47.10
FEATURES             Location/Qualifiers
     source          1..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             398..1189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1263..2213)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2261..2483
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2750..2836)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3000..3034
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3037..3086
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3152..3160
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg
     1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg
     1261 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     1321 agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc
     1381 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     1441 ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     1501 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     1561 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     1621 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     1681 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     1741 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     1801 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     1861 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     1921 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     1981 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     2041 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     2101 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     2161 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     2221 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     2281 catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
     2341 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     2401 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     2461 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     2521 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     2581 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     2641 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     2701 gggtaaatgg cactacaggc gcggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     2761 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     2821 cctttcgttt tatttgatgc ctttaattaa ggctcgggag acctatcggt aataacagtc
     2881 caatctggtg taacttcgga atcgtcccca attattgaac acccttcggg gtgttttttt
     2941 gtttctggtc taccatctcg ttgtgataat agacctgaag tgcctactct ggaaaatctt
     3001 tgacagctag ctcagtccta ggtataatgc tagcagctgt caccggatgt gctttccggt
     3061 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaag gctcgctacg
     3121 agaagattgt tactttcgca gcgttattat ctaaggaggt agtccatggt tagcaaaggc
     3181 gaggcggtta tcaaggagtt tatgcgtttt aaggttcaca tggagggtag catgaatggt
     3241 cacgagttcg agatcgaggg tgaaggcgag ggtcgtccgt acgaaggcac ccagaccgcg
     3301 aagctgaaag tgaccaaggg tggcccgctg ccgttcagct gggacatcct gagcccgcag
     3361 ttcatgtatg gcagccgtgc gtttaccaaa cacccggcgg acattccgga ttactataag
     3421 caaagcttcc cggaaggttt taaatgggag cgtgttatga acttcgaaga tggtggcgcg
     3481 gtgaccgtta cccaggacac cagcctggag gatggcaccc tgatttacaa ggtgaaactg
     3541 cgtggcacca actttccgcc ggatggtccg gttatgcaga agaaaacgat gggttgggaa
     3601 gcgagcaccg agcgtctgta tccggaagat ggcgtgctga agggtgatat caaaatggcg
     3661 ctgcgtctga aggacggtgg ccgttacctg gcggatttta agaccaccta taaagcgaag
     3721 aaaccggtgc aaatgccggg tgcgtacaac gttgaccgta aactggatat taccagccac
     3781 aacgaggatt ataccgtggt tgagcaatat gagcgtagcg agggtcgcca cagcaccggc
     3841 ggcatggacg aactgtataa gggatcctaa taa
//
