LOCUS       sequence-353848-        6169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_5a5a.10
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             277..1467
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(629..648)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(1544..2395)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2382..3221)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3732..4703)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4810..5204
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5242..5328)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5400..5434
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             5443..5454
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta
      241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
      301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
      601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg
      781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc
     1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc
     1501 gtacaattct gaggtccaca cagatagtgt ccgtaggccg gcctcagcct gccgccttgg
     1561 gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca
     1621 gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct
     1681 ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt
     1741 cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca
     1801 cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg
     1861 ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc
     1921 gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga
     1981 tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg
     2041 ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg
     2101 gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta
     2161 ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca
     2221 gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca
     2281 gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt
     2341 gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc
     2401 acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc
     2461 cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg
     2521 acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg
     2581 ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc
     2641 tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg
     2701 atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg
     2761 ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg
     2821 cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg
     2881 ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg
     2941 aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc
     3001 aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca
     3061 atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc
     3121 gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct
     3181 gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg
     3241 cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt
     3301 ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca
     3361 tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca
     3421 gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg
     3481 gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg
     3541 gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca
     3601 ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt
     3661 tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac
     3721 caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt
     3781 gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg
     3841 ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg
     3901 ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc
     3961 tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc
     4021 atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcgggc
     4081 agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc
     4141 tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc
     4201 agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag
     4261 cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg
     4321 gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc
     4381 catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct
     4441 ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc
     4501 tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc
     4561 gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg
     4621 tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc
     4681 tgcaaagtcc tgtcgttctt catcgggcca ccaagcgatt cccacacatt atacgagccg
     4741 gaagcataaa gtgtagctag atccgaagga tgagccgggc tgaatgatcg accgagacag
     4801 gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg
     4861 gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc
     4921 gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca
     4981 gctatccggc ctctggccgg gcatattggg caagggcagc agcgccccac aagggcgctg
     5041 ataaccgcgc ctagtggatt attcttagat aatcatggat ggatttttcc aacaccccgc
     5101 cagcccccgc ccctgctggg tttgcaggtt tgggggcgtg acagttattg caggggttcg
     5161 tgacagttat tgcagggggg cgtgacagtt attgcagggg ttcgtgacag ttagggcgcg
     5221 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     5281 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt
     5341 aaggctcggg agacctatcg gtaataacag tccaatctgg tgtaacttcg gaatcgtcct
     5401 tgacagctag ctcagtccta ggtataatgc tagctactag tgaaagagga gaaatactag
     5461 tatggttagc aaaggcgagg cggttatcaa ggagtttatg cgttttaagg ttcacatgga
     5521 gggtagcatg aatggtcacg agttcgagat cgagggtgaa ggcgagggtc gtccgtacga
     5581 aggcacccag accgcgaagc tgaaagtgac caagggtggc ccgctgccgt tcagctggga
     5641 catcctgagc ccgcagttca tgtatggcag ccgtgcgttt accaaacacc cggcggacat
     5701 tccggattac tataagcaaa gcttcccgga aggttttaaa tgggagcgtg ttatgaactt
     5761 cgaagatggt ggcgcggtga ccgttaccca ggacaccagc ctggaggatg gcaccctgat
     5821 ttacaaggtg aaactgcgtg gcaccaactt tccgccggat ggtccggtta tgcagaagaa
     5881 aacgatgggt tgggaagcga gcaccgagcg tctgtatccg gaagatggcg tgctgaaggg
     5941 tgatatcaaa atggcgctgc gtctgaagga cggtggccgt tacctggcgg attttaagac
     6001 cacctataaa gcgaagaaac cggtgcaaat gccgggtgcg tacaacgttg accgtaaact
     6061 ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc gtagcgaggg
     6121 tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa
//
