LOCUS       sequence-353849-        3227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_5a6.10
FEATURES             Location/Qualifiers
     source          1..3227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             277..1467
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(629..648)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      1577..2122
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2023..2042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2300..2386)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2458..2492
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2501..2512
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta
      241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
      301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
      601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg
      781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc
     1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc
     1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc ctagaaatat tttatctgat
     1561 taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg
     1621 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc
     1681 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac
     1741 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt
     1801 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1861 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     1921 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     1981 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
     2041 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     2101 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt
     2161 atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc
     2221 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat ccggcgcgcc
     2281 cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     2341 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctttaattaa
     2401 ggctcgggag acctatcggt aataacagtc caatctggtg taacttcgga atcgtccttg
     2461 acagctagct cagtcctagg tataatgcta gctactagtg aaagaggaga aatactagta
     2521 tggttagcaa aggcgaggcg gttatcaagg agtttatgcg ttttaaggtt cacatggagg
     2581 gtagcatgaa tggtcacgag ttcgagatcg agggtgaagg cgagggtcgt ccgtacgaag
     2641 gcacccagac cgcgaagctg aaagtgacca agggtggccc gctgccgttc agctgggaca
     2701 tcctgagccc gcagttcatg tatggcagcc gtgcgtttac caaacacccg gcggacattc
     2761 cggattacta taagcaaagc ttcccggaag gttttaaatg ggagcgtgtt atgaacttcg
     2821 aagatggtgg cgcggtgacc gttacccagg acaccagcct ggaggatggc accctgattt
     2881 acaaggtgaa actgcgtggc accaactttc cgccggatgg tccggttatg cagaagaaaa
     2941 cgatgggttg ggaagcgagc accgagcgtc tgtatccgga agatggcgtg ctgaagggtg
     3001 atatcaaaat ggcgctgcgt ctgaaggacg gtggccgtta cctggcggat tttaagacca
     3061 cctataaagc gaagaaaccg gtgcaaatgc cgggtgcgta caacgttgac cgtaaactgg
     3121 atattaccag ccacaacgag gattataccg tggttgagca atatgagcgt agcgagggtc
     3181 gccacagcac cggcggcatg gacgaactgt ataagggatc ctaataa
//
