LOCUS       sequence-353853-        3872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_27_pKD46.10
FEATURES             Location/Qualifiers
     source          1..3872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1246..2196)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2244..2466
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2749..2835)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2999..3033
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3036..3085
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3151..3159
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca
     1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct acttttgttt
     1201 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta
     1261 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     1321 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     1381 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc
     1441 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     1501 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     1561 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     1621 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     1681 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     1741 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     1801 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     1861 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc
     1921 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     1981 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     2041 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     2101 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     2161 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     2221 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     2281 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     2341 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat
     2401 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta
     2461 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc
     2521 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggt
     2581 tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     2641 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     2701 ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat ttgtcctact
     2761 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     2821 ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta ataacagtcc
     2881 aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg tgtttttttg
     2941 tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg gaaaatcttt
     3001 gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg ctttccggtc
     3061 tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg ctcgctacga
     3121 gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt agcaaaggcg
     3181 aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc atgaatggtc
     3241 acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc cagaccgcga
     3301 agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg agcccgcagt
     3361 tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat tactataagc
     3421 aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat ggtggcgcgg
     3481 tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag gtgaaactgc
     3541 gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg ggttgggaag
     3601 cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc aaaatggcgc
     3661 tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat aaagcgaaga
     3721 aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt accagccaca
     3781 acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac agcaccggcg
     3841 gcatggacga actgtataag ggatcctaat aa
//
