LOCUS       sequence-353855-        3942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_47_pKD46.10
FEATURES             Location/Qualifiers
     source          1..3942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             398..1189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1316..2266)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2314..2536
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2819..2905)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3069..3103
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3106..3155
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3221..3229
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg
     1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct
     1261 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga
     1321 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg
     1381 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac
     1441 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta
     1501 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt
     1561 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat
     1621 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt
     1681 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa
     1741 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct
     1801 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag
     1861 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata
     1921 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact
     1981 ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct
     2041 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag
     2101 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg
     2161 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc
     2221 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt
     2281 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc
     2341 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa
     2401 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc
     2461 aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca
     2521 gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa
     2581 cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg
     2641 caagctcggt tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     2701 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     2761 agtgaatggg ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat
     2821 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2881 tcgactgagc ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta
     2941 ataacagtcc aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg
     3001 tgtttttttg tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg
     3061 gaaaatcttt gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg
     3121 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg
     3181 ctcgctacga gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt
     3241 agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc
     3301 atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc
     3361 cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg
     3421 agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat
     3481 tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat
     3541 ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag
     3601 gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg
     3661 ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc
     3721 aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat
     3781 aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt
     3841 accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac
     3901 agcaccggcg gcatggacga actgtataag ggatcctaat aa
//
