LOCUS       sequence-353859-        3681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_67.10
FEATURES             Location/Qualifiers
     source          1..3681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(193..198,199..215,216..221)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             410..943
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             complement(1071..2021)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2069..2291
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2558..2644)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2808..2842
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        2845..2894
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             2960..2968
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
      241 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      301 gttttcatgg cttgttatga ctgttttttt gtacagccta tgcctcgggc atccaagcag
      361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
      481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
      541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
      601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
      661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
      721 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac
      781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
      841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
      901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
      961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact
     1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccggccggcc tcagatcctt
     1081 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     1141 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     1201 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     1261 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     1321 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     1381 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     1441 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     1501 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     1561 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     1621 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     1681 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     1741 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     1801 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     1861 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     1921 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     1981 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     2041 tgattttaat cactatacca attgagatgg gctagtcaat gataattaca tgtccttttc
     2101 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     2161 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     2221 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     2281 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     2341 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     2401 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     2461 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     2521 ctacaggcgc ggcgcgccca gctgtctagg gcggcggatt tgtcctactc aggagagcgt
     2581 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     2641 tttgatgcct ttaattaagg ctcgggagac ctatcggtaa taacagtcca atctggtgta
     2701 acttcggaat cgtccccaat tattgaacac ccttcggggt gtttttttgt ttctggtcta
     2761 ccatctcgtt gtgataatag acctgaagtg cctactctgg aaaatctttg acagctagct
     2821 cagtcctagg tataatgcta gcagctgtca ccggatgtgc tttccggtct gatgagtccg
     2881 tgaggacgaa acagcctcta caaataattt tgtttaaggc tcgctacgag aagattgtta
     2941 ctttcgcagc gttattatct aaggaggtag tccatggtta gcaaaggcga ggcggttatc
     3001 aaggagttta tgcgttttaa ggttcacatg gagggtagca tgaatggtca cgagttcgag
     3061 atcgagggtg aaggcgaggg tcgtccgtac gaaggcaccc agaccgcgaa gctgaaagtg
     3121 accaagggtg gcccgctgcc gttcagctgg gacatcctga gcccgcagtt catgtatggc
     3181 agccgtgcgt ttaccaaaca cccggcggac attccggatt actataagca aagcttcccg
     3241 gaaggtttta aatgggagcg tgttatgaac ttcgaagatg gtggcgcggt gaccgttacc
     3301 caggacacca gcctggagga tggcaccctg atttacaagg tgaaactgcg tggcaccaac
     3361 tttccgccgg atggtccggt tatgcagaag aaaacgatgg gttgggaagc gagcaccgag
     3421 cgtctgtatc cggaagatgg cgtgctgaag ggtgatatca aaatggcgct gcgtctgaag
     3481 gacggtggcc gttacctggc ggattttaag accacctata aagcgaagaa accggtgcaa
     3541 atgccgggtg cgtacaacgt tgaccgtaaa ctggatatta ccagccacaa cgaggattat
     3601 accgtggttg agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa
     3661 ctgtataagg gatcctaata a
//
