LOCUS       sequence-35388-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, 
            Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, 
            Antosiewicz-Bourget J, Teng JM, Thomson JA
  TITLE     Chemically defined conditions for human iPSC derivation and culture.
  JOURNAL   Nat Methods. 2011 May;8(5):424-9. doi: 10.1038/nmeth.1593. Epub 2011
            Apr 10.
  PUBMED    21478862
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1593"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "424-9"
COMMENT     Sequence Label: pET-3c-rhVTN-N
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             99..121
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag
      121 atatacatat ggtgactcgc ggggatgtgt tcactatgcc ggaggatgag tacacggtct
      181 atgacgatgg cgaggagaaa aacaatgcca ctgtccatga acaggtgggg ggcccctccc
      241 tgacctctga cctccaggcc cagtccaaag ggaatcctga gcagacacct gttctgaaac
      301 ctgaggaaga ggcccctgcg cctgaggtgg gcgcctctaa gcctgagggg atagactcaa
      361 ggcctgagac ccttcatcca gggagacctc agcccccagc agaggaggag ctgtgcagtg
      421 ggaagccctt cgacgccttc accgacctca agaacggttc cctctttgcc ttccgagggc
      481 agtactgcta tgaactggac gaaaaggcag tgaggcctgg gtaccccaag ctcatccgag
      541 atgtctgggg catcgagggc cccatcgatg ccgccttcac ccgcatcaac tgtcagggga
      601 agacctacct cttcaagggt agtcagtact ggcgctttga ggatggtgtc ctggaccctg
      661 attacccccg aaatatctct gacggcttcg atggcatccc ggacaacgtg gatgcagcct
      721 tggccctccc tgcccatagc tacagtggcc gggagcgggt ctacttct
//
