LOCUS       sequence-35389-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, 
            Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, 
            Antosiewicz-Bourget J, Teng JM, Thomson JA
  TITLE     Chemically defined conditions for human iPSC derivation and culture.
  JOURNAL   Nat Methods. 2011 May;8(5):424-9. doi: 10.1038/nmeth.1593. Epub 2011
            Apr 10.
  PUBMED    21478862
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1593"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "424-9"
COMMENT     Sequence Label: pET-3c-rhVTN-NC
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
      121 gatatacata tggtgactcg cggggatgtg ttcactatgc cggaggatga gtacacggtc
      181 tatgacgatg gcgaggagaa aaacaatgcc actgtccatg aacaggtggg gggcccctcc
      241 ctgacctctg acctccaggc ccagtccaaa gggaatcctg agcagacacc tgttctgaaa
      301 cctgaggaag aggcccctgc gcctgaggtg ggcgcctcta agcctgaggg gatagactca
      361 aggcctgaga cccttcatcc agggagacct cagcccccag cagaggagga gctgtgcagt
      421 gggaagccct tcgacgcctt caccgacctc aagaacggtt ccctctttgc cttccgaggg
      481 cagtactgct atgaactgga cgaaaaggca gtgaggcctg ggtaccccaa gctcatccga
      541 gatgtctggg gcatcgaggg ccccatcgat gccgccttca cccgcatcaa ctgtcagggg
      601 aagacctacc tcttcaaggg tagtcagtac tggcgctttg aggatggtgt cctggaccct
      661 gattaccccc gaaatatctc tgacggcttc gatggcatcc cggacaacgt ggatgcagcc
      721 ttggccctcc ctgcccatag ctacagtggc cgggagcggg tctacttctt caaggggaaa
      781 cagtactggg agtaccagtt cca
//
