LOCUS       sequence-353893-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pH-sensitive RFP pHlorina for detection of efferocytosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Raymond MH, Davidson AJ, Shen Y, Tudor DR, Lucas CD, Morioka S, 
            Perry JSA, Krapivkina J, Perrais D, Schumacher LJ, Campbell RE, Wood
            W, Ravichandran KS
  TITLE     Live cell tracking of macrophage efferocytosis during Drosophila 
            embryo development in vivo.
  JOURNAL   Science. 2022 Mar 11;375(6585):1182-1187. doi: 
            10.1126/science.abl4430. Epub 2022 Mar 10.
  PUBMED    35271315
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abl4430"; journalName: "Science"; date: 
            "2022-03-11- 11"; volume: "375"; issue: "6585"; pages: "1182-1187"
COMMENT     Sequence Label: pBAD-pHlorina
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKQSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGYEASEERMYPEDGALK
                     SECKERLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERT
                     EGRHSTGGMDELYK"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggttta cattaagcac
      241 ccagccgaca tccccgacta cttcaagcag tccttccccg agggcttcag gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
      361 ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctatgaggcc tccgaggagc ggatgtaccc cgaggacggc
      481 gccctgaaga gcgagtgtaa ggagaggctg aagctgaagg acggcggcca ctacgccgcc
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
      601 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcactgagg gccgccactc caccggcggc atggacgagc tgtacaagta a
//
