LOCUS       sequence-353897-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     402..421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(628..633,634..650,651..656)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             704..1894
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1056..1075)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2068..2087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2069..2087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2515..2602,1..501)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
       61 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      541 tgctggcctt ttgctcacat gttctttcct gcgttatcca cagaatcagg ggataacgca
      601 ggaaagaaca tgaattaatt ctcatgtttg acagcttatc atcgattagc tttaatgcgg
      661 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
      721 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
      781 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
      841 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
      901 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
      961 ctacgcgatc atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc
     1021 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1081 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1141 aggccccgtg gccgggggac tgttgggcgc catctcctta catgcaccat tccttgcggc
     1201 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1261 gggagagcgc cgacccatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1321 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1381 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     1441 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     1501 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     1561 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     1621 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     1681 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     1741 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     1801 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     1861 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     1921 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     1981 accaaccctt gatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     2041 agcctgattg ggagaaaata aaatatcccc tatagtgagt cgtattacat ggtcatagct
     2101 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2161 aatatatcat catgcctcct ctagaccaaa gctggtctcg gcaattgcta gtaatattgg
     2221 tggcaaacag gctcttgaga cggttcagcg cctccttcca gttctctgtc aagcccacgg
     2281 actcacccca gatcaagttg tagcgattgc tagtaatggg ggtggcaaac aggctcttga
     2341 gactgttcag cgccttctac cagttctctg tcaagcccac ggcctgacgc ccgagcaagt
     2401 tgtagcgatt tgagaccata tctggtcgag ccacccatga ccaaaatccc ttaacgtgag
     2461 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2521 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2581 ggtttgtttg ccggatcaag ag
//
