LOCUS       sequence-353899-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..129)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(111..129)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(134..150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(134..150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      558..1146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1047..1066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1273..1278,1279..1295,1296..1301)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1349..2539
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1701..1720)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta tgtcgagcta
       61 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc cctatagtga
      121 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      181 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca aagctggtct
      241 caggctatcg ctacaacttg ctcgggcgtc aggccgtggg cttgacagag aactggtaga
      301 aggcgctgaa cagtctcaag agcctgtttg ccacccccat tactagcaat cgctacaact
      361 tgatctgggg tgagtccgtg ggcttgacag agaactggaa ggaggcgctg aaccgtctca
      421 agagcctgtt tgccaccaat attactagca atccgagacc atatctggtc gagccaccca
      481 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
      541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1201 cacatgttct ttcctgcgtt atccacagaa tcaggggata acgcaggaaa gaacatgaat
     1261 taattctcat gtttgacagc ttatcatcga ttagctttaa tgcggtagtt tatcacagtt
     1321 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     1381 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     1441 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     1501 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     1561 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     1621 gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc
     1681 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     1741 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     1801 gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg tgctcaacgg
     1861 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgacc
     1921 catgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     1981 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     2041 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     2101 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     2161 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     2221 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     2281 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     2341 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     2401 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     2461 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     2521 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     2581 agaattggag ccaatcaatt ctt
//
