LOCUS       sequence-35390-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Sleight SC, Bartley BA, Lieviant JA, Sauro HM
  TITLE     Designing and engineering evolutionary robust genetic circuits
  JOURNAL   J Biol Eng. 2010 Nov 1;4:12
  PUBMED    21040586
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-11-1"; volume: "4"; pages: "1"
COMMENT     Sequence Label: SS13 lac-inducible GFP reporter circuit
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..764
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(81..109)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     704..724
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggaggacgg aacatttcac acatactaga gtcacacagg aaagtactag atgcgtaaag
       61 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      121 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      181 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      241 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      301 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      361 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      421 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      481 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      541 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      601 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      661 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      721 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaa
//
