LOCUS       sequence-353900-       10184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM5 (with a C-terminal V5 tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM5-V5
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5333..5374
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(5379..5403)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5434..6022
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5487..5507)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5905..5916)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6036..6535
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6071..6094)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6468..6487
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6556..7155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6556..6575)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7052..7072
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7293..7526
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7598..7719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7635..7654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7689..7708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7759..7894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7914..7933)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7915..7933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7943..7960)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7943..7959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7952..7974)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8101..8556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8188..8207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8398..8419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8582..8686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8687..8755,8756..9547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8905..8924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9718..10184,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatggccca
     3181 agggctagaa gtggccctca cagacctcca gagctccaga aataatgtgc ggcaccacac
     3241 ggaggagatc actgtggacc acctgcttgt tcgccggggc caggccttca acctcaccct
     3301 gtacttcagg aaccggagct tccagccagg cctggacaac atcatcttcg tggttgaaac
     3361 tggaccgctg ccagacctgg ccttggggac tcgggctgtg ttcagcctgg cacgccatca
     3421 cagccccagc ccctggattg cctggctgga gaccaatggg gccacctcca cagaggtgag
     3481 cttgtgcgct cctcccaagg cggccgtggg tcggtacctc ttgaaaatcc acatcgactc
     3541 cttccagggg tctgtgacgg cctaccagct aggggagttc atcctgcttt tcaatccctg
     3601 gtgcccagag gatgctgtct acttggacag tgaaccccag aggcaggagt atgtcatgaa
     3661 tgattatggc ttcatctacc aaggcagcaa gaactggatc cgcccatgtc cctggaacta
     3721 tggacagttt gaagacaaaa tcatagacat ctgcctgaag ctgctagaca agagcctgca
     3781 cttccagact gacccagcca cagactgtgc tctgcgggga agccccgtct acgtcagcag
     3841 agtggtgtgt gccatgatca acagcaatga tgataatggg gtgctcaatg gaaactggag
     3901 tgagaattac acagacggcg ccaaccctgc ggagtggacg ggcagcgtgg ccatcctgaa
     3961 gcagtggaac gccacaggct gccagcccgt gcgctacggg caatgctggg tctttgctgc
     4021 cgtcatgtgc acagtgatga ggtgtctggg gatccctacc cgtgtgatca ccaacttcga
     4081 ctctggccac gatacagatg gaaacctgat catagatgag tactatgaca acacaggcag
     4141 gattttgggg aataagaaga aggatactat ctggaacttc catgtctgga atgagtgctg
     4201 gatggcccgg aaggatctgc cccctgcata tggaggctgg caggtgctgg acgccacacc
     4261 tcaggagatg agcaacggcg tctactgctg tggccctgcc tctgtcagag ccatcaaaga
     4321 aggagaagtg gacctgaact atgacacgcc ctttgtgttt tcgatggtga atgctgactg
     4381 catgtcctgg ctcgtccagg gagggaagga gcagaagctt caccaggaca cgagttctgt
     4441 tggcaatttt atcagcacaa agagcatcca gagtgacgag cgggatgaca tcacagagaa
     4501 ctacaagtat gaagaaggat ccctccagga gaggcaggtg tttctgaagg ctctgcagaa
     4561 gctgaaggct agaagcttcc atggctccca aagaggagca gagttgcaac cttccaggcc
     4621 cacatcactg agccaggaca gccctcggag cctgcataca ccttcccttc gacccagtga
     4681 tgtggtgcaa gtctccctga aattcaagct gctcgacccg cccaacatgg gccaggatat
     4741 atgctttgtc ctgctggccc tcaacatgtc ctcccagttc aaggacctca aagtgaacct
     4801 gagtgcccag tctctgctgc acgatggcag ccccctgtcc ccattctggc aggacacagc
     4861 gttcatcaca ctctctccta aagaagcaaa gacctacccc tgcaaaatct cctattccca
     4921 gtacagccag tacctgtcaa cagacaagct gatccgcatc agtgccctgg gtgaagagaa
     4981 aagcagtcct gagaaaatcc tggtgaacaa gatcatcacc ttatcttatc caagcatcac
     5041 gattaatgtt ctaggagcag ccgttgtgaa ccagccactc tccatacagg tgatattttc
     5101 aaaccccctc tcggagcagg ttgaggactg tgtgctgact gtggaaggaa gtggcctctt
     5161 caagaaacag cagaaagtct tccttggagt cctcaaaccc caacaccaag caagcatcat
     5221 tctggagacc gtccccttca agagtggaca aaggcagatc caagctaata tgagaagcaa
     5281 caagtttaag gacattaagg gttacaggaa tgtttatgta gactttgcat taggtaagcc
     5341 tatccctaac cctctcctcg gtctcgattc tacgtgagac ccagctttct tgtacaaagt
     5401 ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt
     5461 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5521 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5581 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5641 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5701 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5761 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5821 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5881 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5941 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6001 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     6061 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     6121 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     6181 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     6241 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     6301 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     6361 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     6421 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     6481 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     6541 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6601 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6661 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6721 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6781 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6841 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6901 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6961 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7021 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7081 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7141 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     7201 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     7261 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     7321 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7381 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7441 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7501 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7561 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7621 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7681 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7741 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7801 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7861 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     7921 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7981 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8041 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     8101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8161 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8221 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8281 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8401 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8461 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8521 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     8581 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8641 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8701 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8761 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8821 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8881 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8941 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9001 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9061 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9121 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9181 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9241 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9301 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9361 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9421 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9481 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9541 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9601 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9661 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9721 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9781 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9841 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9901 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9961 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10021 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10081 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10141 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggag
//
