LOCUS       sequence-353906-        8738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aTc inducible MCP-SoxS double mutant expression for CRISPRa in 
            E.coli. Contains constitutive dCas9 and scRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Tickman BI, Burbano DA, Chavali VP, Kiattisewee C, Fontana J, 
            Khakimzhan A, Noireaux V, Zalatan JG, Carothers JM
  TITLE     Multi-layer CRISPRa/i circuits for dynamic genetic programs in 
            cell-free and bacterial systems.
  JOURNAL   Cell Syst. 2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 
            10.1016/j.cels.2021.10.008.
  PUBMED    34800362
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 
            10.1016/j.cels.2021.10.008."
COMMENT     Sequence Label: pCK389.AAV
FEATURES             Location/Qualifiers
     source          1..8738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(220..298)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(594..620,621..623,624..3110,3111..3113,3114..4700)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      4724..4795
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4811..4838
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4891..5517)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     5422..5441
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        5533..5588
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    5569..5587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             5600..5611
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(6513..7058)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6593..6612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7166..7200
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        7284..7302
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             7325..7354
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7370..7387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7443..7460)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7613..7659
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(7877..7971)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7992..8651)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8566..8585
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8652..8738,1..16))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg acgtcttaag ttacgaaatc
       61 atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga atgataaaag
      121 gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc gcttgttttt
      181 ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg gtgccacttt
      241 ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga atggttccaa
      301 caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag aaatttatca
      361 gccataaaac aatacttaat actatagaat gataacaaaa taaactactt tttaaaagaa
      421 ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa gagatatttt
      481 gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac tattattgaa
      541 atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca aaaatggata
      601 agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg gtgatcactg
      661 atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta
      721 tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc
      781 gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac
      841 aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag
      901 agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag
      961 tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg
     1021 tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta
     1081 agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca
     1141 aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg
     1201 caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag
     1261 aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg
     1321 ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta
     1381 aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg
     1441 gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt
     1501 cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta
     1561 aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac
     1621 ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata
     1681 ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa
     1741 tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac
     1801 ggacctttga caacggctct attccccatc aaattcactt gggtgagctg catgctattt
     1861 tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa
     1921 tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat agtcgttttg
     1981 catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg
     2041 ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc
     2101 caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg
     2161 aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg
     2221 aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc
     2281 aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag
     2341 ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag
     2401 ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat
     2461 tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct
     2521 ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt
     2581 ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata ttagattttt
     2641 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga
     2701 catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac
     2761 atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag
     2821 ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa
     2881 tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac
     2941 gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa
     3001 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt
     3061 atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat gccattgttc
     3121 cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa
     3181 atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt
     3241 ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag
     3301 ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg
     3361 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat
     3421 acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag
     3481 tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc
     3541 atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa
     3601 aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg
     3661 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca
     3721 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa
     3781 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag
     3841 tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag
     3901 gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta
     3961 aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag
     4021 tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt
     4081 tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag
     4141 aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc
     4201 tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag
     4261 gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg
     4321 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata
     4381 agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag
     4441 cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac
     4501 gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg
     4561 cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt
     4621 tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga
     4681 gtcagctagg aggtgactaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc
     4741 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     4801 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctaga gcgcccggaa
     4861 gagagtcaat tcagggtggt gaatctgcag ttaagaccca ctttcacatt taagttgttt
     4921 ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg
     4981 atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc
     5041 cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg
     5101 ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa
     5161 ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac
     5221 ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa
     5281 aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat
     5341 ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg
     5401 ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc
     5461 attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag acatcattaa
     5521 ttcctaattt ttgttgacac tctatcgttg atagagttat tttaccactc cctatcagtg
     5581 atagagaaaa gaattcatta aagaggagaa aggtaccatg gggcccgctt ctaactttac
     5641 tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt
     5701 cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac
     5761 ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc
     5821 taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa
     5881 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     5941 tccctcagca atcgcagcaa actccggcat ctacggtggc ggaggtagca tgtcccatca
     6001 gaaaattatt caggatctta tcgcatggat tgacgagcat attgaccagc cgcttaacat
     6061 tgatgtagtc gcaaaaaaat caggctattc aaagtggtac ttgcaacgaa tgttccgcac
     6121 ggtgacgcat cagacgcttg gcgattacat tcgccaacgc cgcctgttac tggccgccgt
     6181 tgagttgcgc accaccgagc gtccgatttt tgatatcgca atggacctgg gttatgtctc
     6241 gcagcagacc ttctcccgcg ttttcgcgcg gcagtttgat cgcactcccg cggattatcg
     6301 ccaccgcctg taagcggccg ccacgcaaaa aaccccgctt cggcggggtt ttttcgccct
     6361 agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     6421 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     6481 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     6541 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     6601 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     6661 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     6721 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     6841 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     6901 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     6961 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     7021 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
     7081 taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     7141 ccatacgata taagttgtta ctagattgac agctagctca gtcctaggta taatactagt
     7201 ggggccacta gggacaggat gttttagagc tagaaatagc aagttaaaat aaggctagtc
     7261 cgttatcaac ttgaaaaagt ggcacatgag gatcacccat gtgctttttt tgaagcttgg
     7321 gcccgaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca
     7381 tcatcattga gtttaaacgg tctccagctt ggctgttttg gcggatgaga gaagattttc
     7441 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     7501 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     7561 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     7621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaactg
     7681 gatccttact cgagtctaga ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7861 aaaaactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     7921 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     7981 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     8041 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     8101 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     8161 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     8221 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     8281 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     8341 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     8401 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     8461 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     8521 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     8581 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     8641 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     8701 ggtagtgatc ttatttcatt atggtgaaag ttggaacc
//
