LOCUS       sequence-353912-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM1 (with a C-terminal Flag tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM1-Flag
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5630..5653
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5658..5682)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5713..6301
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5766..5786)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6184..6195)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6315..6814
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6350..6373)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6747..6766
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6835..7434
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6835..6854)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7331..7351
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7572..7805
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7877..7998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7914..7933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7968..7987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8038..8173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8193..8212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8194..8212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8222..8239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8222..8238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8231..8253)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8380..8835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8467..8486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8677..8698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8861..8965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8966..9034,9035..9826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9184..9203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9997..10463,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatgatgga
     3181 tgggccacgt tccgatgtgg gccgttgggg tggcaacccc ttgcagcccc ctaccacgcc
     3241 atctccagag ccagagccag agccagacgg acgctctcgc agaggaggag gccgttcctt
     3301 ctgggctcgc tgctgtggct gctgttcatg ccgaaatgcg gcagatgacg actggggacc
     3361 tgaaccctct gactccaggg gtcgagggtc cagctctggc actcgaagac ctggctcccg
     3421 gggctcagac tcccgccggc ctgtatcccg gggcagcggt gtcaatgcag ctggagatgg
     3481 caccatccga gagggcatgc tagtagtgaa cggtgtggac ttgctgagct cgcgctcgga
     3541 ccagaaccgc cgagagcacc acacagacga gtatgagtac gacgagctga tagtgcgccg
     3601 cgggcagcct ttccatatgc tcctcctcct gtcccggacc tatgaatcct ctgatcgcat
     3661 cacccttgag ttactcatcg gaaacaaccc cgaggtgggc aagggcacgc acgtgatcat
     3721 cccagtgggc aaggggggca gtggaggctg gaaagcccag gtggtcaagg ccagtgggca
     3781 gaatctgaac ctgcgggtcc acacttcccc caacgccatc atcggcaagt ttcagttcac
     3841 agtccgcaca caatcagacg ctggggagtt ccagttgccc tttgaccccc gcaatgagat
     3901 ctacatcctc ttcaacccct ggtgcccaga ggacattgtg tacgtggacc atgaggattg
     3961 gcggcaggag tatgttctta atgagtctgg gagaatttac tacgggaccg aagcacagat
     4021 tggtgagcgg acctggaact acggccagtt tgaccacggg gtgctggatg cctgcttata
     4081 catcctggac cggcggggga tgccatatgg aggccgtgga gacccagtca atgtctcccg
     4141 ggtcatctct gccatggtga actccctgga tgacaatgga gtcctgattg ggaactggtc
     4201 tggtgattac tcccgaggca ccaacccatc agcgtgggtg ggcagcgtgg agatcctgct
     4261 tagctaccta cgcacgggat attccgtccc ctatggccag tgctgggtct ttgctggcgt
     4321 gaccaccaca gtgctgcgct gcctgggtct ggccacccgt actgtcacca acttcaactc
     4381 cgcccacgac acagacacat cccttaccat ggacatctac ttcgacgaga acatgaagcc
     4441 cctggagcac ctgaaccatg attctgtctg gaacttccat gtgtggaacg actgctggat
     4501 gaagaggccg gatctgccct cgggctttga tgggtggcag gtggtggatg ccacacccca
     4561 agagactagc agtggcatct tctgctgcgg cccctgctct gtggagtcca tcaagaatgg
     4621 cctggtctac atgaagtacg acacgccttt catttttgct gaggtgaata gtgacaaggt
     4681 gtactggcag cggcaggatg atggcagctt caagattgtt tatgtggagg agaaggccat
     4741 cggcacactc attgtcacaa aggccatcag ctccaacatg cgggaggaca tcacctacct
     4801 ctataagcac ccagaaggct cagacgcaga gcggaaggca gtagagacag cagcagccca
     4861 cggcagcaaa cccaatgtgt atgccaaccg gggctcagcg gaggatgtgg ccatgcaggt
     4921 ggaggcacag gacgcggtga tggggcagga tctgatggtc tctgtgatgc tgatcaatca
     4981 cagcagcagc cgccgcacag tgaaactgca cctctacctc tcagtcactt tctatactgg
     5041 tgtcagtggt accatcttca aggagaccaa gaaggaagtg gagctggcac caggggcctc
     5101 ggaccgtgtg accatgccag tggcctacaa ggaataccgg ccccatcttg tggaccaggg
     5161 ggccatgctg ctcaatgtct caggccacgt caaggagagc gggcaggtgc tggccaagca
     5221 gcacaccttc cgtctgcgca ccccagacct ctccctcacg ttactgggag cagcagtggt
     5281 tggccaggag tgtgaagtac agattgtctt caagaacccc cttcccgtca ccctcaccaa
     5341 tgtcgtcttc cggctcgaag gctctgggtt acagaggccc aagatcctca acgttgggga
     5401 cattggaggc aatgaaacag tgacactgcg ccagtcgttt gtgcctgtgc gaccaggccc
     5461 ccgccagctc attgccagct tggacagccc acagctctcc caggtgcacg gtgtcatcca
     5521 ggtggatgtg gccccagccc ctggggatgg gggcttcttc tcagacgctg gaggtgacag
     5581 tcacttagga gagaccatcc ctatggcatc tcgaggtgga gctggcggcg actacaaaga
     5641 cgatgacgac aagtgagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     5701 cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5761 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5821 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5881 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5941 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6001 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6061 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6121 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6181 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6241 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6301 cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     6361 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     6421 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     6481 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     6541 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     6601 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     6661 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     6721 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     6781 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     6841 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6901 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6961 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7021 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7081 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7141 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7201 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7261 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7321 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7381 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     7441 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc
     7501 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7561 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     7621 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7681 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7741 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7801 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7861 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7981 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8161 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8221 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8281 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8341 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     8401 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8461 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8521 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8581 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8641 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8701 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8761 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8821 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8941 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9001 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9061 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9121 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9181 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9241 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9301 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9361 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9421 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9481 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9541 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9601 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9661 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9721 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9781 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9841 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9901 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9961 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10021 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10081 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10141 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10201 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10261 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10321 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10381 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10441 aagcggcagg gtcggaacag gag
//
