LOCUS       sequence-353914-        4461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A7_STOP
FEATURES             Location/Qualifiers
     source          1..4461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2018..2117)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2134..2153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2135..2153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2158..2174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2158..2174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2276..2295)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2287..3096
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2354..2373)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3243..3831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3732..3751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3985..4002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4161..4188)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4280..4366)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4415..4437
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4429..4446
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4430..4446
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gaagctgcag
      121 ggagcacacc tgaggaagcc tgtgacacca gacctgctga tgaccccttc tgatcagggc
      181 gacgtggatc tggacgtgga ttttgccgcc cacagaggca actggacagg caagctggac
      241 ttcctgctga gctgcatcgg ctactgcgtg ggcctgggca acgtgtggag gttcccatac
      301 cgcgcctata ccaatggcgg cggcgccttt ctggtgccct acttcctgat gctggccatc
      361 tgcggcatcc ctctgttctt tctggagctg tctctgggac agtttagctc cctgggacca
      421 ctggccgtgt ggaagatcag ccctctgttc aagggagcag gagcagcaat gctgctgatc
      481 gtgggcctgg tggccatcta ctataacatg atcatcgcct acgtgctgtt ttatctgttc
      541 gccagcctga catccgacct gccctgggag cactgcggca actggtggaa taccgagctg
      601 tgcctggagc accgggtgag caaggatggc aacggcgccc tgcctctgaa tctgacctgc
      661 acagtgagcc catccgagga gtactggtcc agatatgtgc tgcacatcca gggaagccag
      721 ggaatcggat ccccaggcga gatcagatgg aatctgtgcc tgtgcctgct gctggcctgg
      781 gtcatcgtgt tcctgtgcat cctgaagggc gtgaagtcta gcggcaaggt ggtgtacttt
      841 accgccacat tcccctatct gatcctgctg atgctgctgg tgcggggagt gacactgcca
      901 ggagcctgga agggcatcca gttttacctg accccccagt tccaccacct gctgtcctct
      961 aaagtgtgga tcgaggccgc cctgcagatc ttttatagcc tgggagtggg atttggagga
     1021 ctgctgacat tcgcctccta caacaccttt caccagaata tctatagaga cacattcatc
     1081 gtgaccctgg gcaatgccat cacctccatc ctggccggct ttgccatctt ctctgtgctg
     1141 ggctacatga gccaggagct gggcgtgcct gtggatcagg tggcaaaggc aggaccagga
     1201 ctggccttcg tggtgtatcc ccaggccatg acaatgctgc ccctgtctcc tttttggagc
     1261 ttcctgttct ttttcatgct gctgaccctg ggcctggact cccagtttgc cttcctggag
     1321 accatcgtga cagccgtgac cgatgagttt ccatactatc tgaggcccaa gaaggccgtg
     1381 ttcagcggcc tgatctgcgt ggccatgtac ctgatgggcc tgatcctgac cacagacggc
     1441 ggcatgtact ggctggtgct gctggacgat tattctgcca gctttggcct gatggtggtg
     1501 gtcatcacca catgcctggc cgtgaccagg gtgtacggca tccagcggtt ctgtagagat
     1561 atccacatga tgctgggctt caagcctggc ctgtattttc gcgcatgctg gctgttcctg
     1621 tccccagcca ccctgctggc cctgatggtg tactctatcg tgaagtatca gccctccgag
     1681 tacggctctt ataggtttcc accttgggca gagctgctgg gaatcctgat gggactgctg
     1741 agctgtctga tgatccctgc aggaatgctg gtggccgtgc tgagggagga gggatccctg
     1801 tgggagcggc tgcagcaggc ctctagacct gccatggact ggggcccaag cctggaggag
     1861 aaccgcacag gaatgtacgt ggccaccctg gcaggatccc agtctccaaa gccactgatg
     1921 gtgcacatga ggaagtatgg cggcatcaca tccttcgaga ataccgccat cgaggtggat
     1981 cgcgagatcg ccgaggagga ggagtctatg atgtaagacc cagctttctt gtacaaagtt
     2041 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2101 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2161 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2221 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2281 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2341 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2401 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2461 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2521 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2581 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2641 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2701 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2761 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2821 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2881 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2941 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3001 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3061 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3121 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3181 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3361 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3601 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3841 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3901 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3961 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4021 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4081 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4141 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4201 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4261 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4321 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4381 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4441 gccagtctta agctcgggcc c
//
