LOCUS       sequence-353916-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..46)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(28..46)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(51..67)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(51..67)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      475..1063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     964..983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1190..1195,1196..1212,1213..1218)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1266..2456
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1618..1637)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcctgattgg gagaaaataa aatatcccct atagtgagtc gtattacatg gtcatagctg
       61 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      121 atatatcatc atgcctcctc tagaccaaag ctggtctcga gccagtaata ttggtggcaa
      181 acaggctctt gagacggttc agcgcctcct tccagttctc tgtcaagccc acggactcac
      241 cccagatcaa gttgtagcga ttgctagtaa tgggggtggc aaacaggctc ttgagactgt
      301 tcagcgcctt ctaccagttc tctgtcaagc ccacggcctg acgcccgagc aagttgtagc
      361 gattgcaagt tgagaccata tctggtcgag ccacccatga ccaaaatccc ttaacgtgag
      421 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1141 cacagaatca ggggataacg caggaaagaa catgaattaa ttctcatgtt tgacagctta
     1201 tcatcgatta gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
     1261 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
     1321 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg
     1381 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
     1441 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
     1501 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc
     1561 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     1621 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     1681 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     1741 tacatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     1801 tcctaatgca ggagtcgcat aagggagagc gccgacccat gcccttgaga gccttcaacc
     1861 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     1921 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     1981 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     2041 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     2101 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     2161 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     2221 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     2281 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     2341 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     2401 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     2461 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     2521 gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta tgtcgagcta
     2581 ttttttgact tactggggat caa
//
