LOCUS       sequence-353919-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     992..1008
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     992..1008
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1013..1032
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1013..1031
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1206..2396)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2025..2044
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2444..2449,2450..2466,2467..2472))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2599..2600,1..587))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
       61 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      421 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      601 tcttttctac ggggtctgac gctcagtgga acgacgcgta actcacgtta agggattttg
      661 gtcatgggtg gctcgaccag atatggtctc aggaagcaat cgctacaact tgctcgggcg
      721 tcaggccgtg ggcttgacag agaactggta gaaggcgctg aacagtctca agagcctgtt
      781 tgccaccccc attactagca atcgctacaa cttgatctgg ggtgagtccg tgggcttgac
      841 agagaactgg aaggaggcgc tgaaccgtct caagagcctg tttgccacca atattacttc
      901 gagaccagct ttggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca
      961 tcagagattt tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga
     1021 ctcactatag gggatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     1081 atagctcgac atactgttct tccccgatca agggttggtt tgcgcattca cagttctccg
     1141 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
     1201 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     1261 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     1321 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc
     1381 gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     1441 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     1501 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg
     1561 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     1621 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     1681 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     1741 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     1801 gggttgaagg ctctcaaggg catgggtcgg cgctctccct tatgcgactc ctgcattagg
     1861 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgt
     1921 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     1981 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     2041 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     2101 agaatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
     2161 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
     2221 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
     2281 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
     2341 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
     2401 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag ctaatcgatg
     2461 ataagctgtc aaacatgaga attaattcat gttctttcct gcgttatccc ctgattctgt
     2521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2581 ggccgcgttg ctggcgtttt
//
