LOCUS       sequence-353922-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(100..105,106..122,123..128))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(255..843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(335..354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1248..1264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1248..1264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1269..1288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1269..1287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1462..2600,1..52))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2281..2300
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
       61 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctaa tcgatgataa
      121 gctgtcaaac atgagaatta attcatgttc tttcctgcgt tatcccctga ttctgtggat
      181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc
      901 acgttaaggg attttggtca tgggtggctc gaccagatat ggtctcaaga ggcaatcgct
      961 acaacttgct cgggcgtcag gccgtgggct tgacagagaa ctggtagaag gcgctgaaca
     1021 gtctcaagag cctgtttgcc acccccatta ctagcaatcg ctacaacttg atctggggtg
     1081 agtccgtggg cttgacagag aactggaagg aggcgctgaa ccgtctcaag agcctgtttg
     1141 ccaccaatat tggaacgaga ccagctttgg tctagaggag gcatgatgat atatttttat
     1201 cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgccag gaaacagcta
     1261 tgaccatgta atacgactca ctatagggga tattttattt tctcccaatc aggcttgatc
     1321 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatcaaggg ttggtttgcg
     1381 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     1441 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     1501 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
     1561 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
     1621 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     1681 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     1741 gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc
     1801 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     1861 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     1921 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     1981 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     2041 ccggaaggag ctgactgggt tgaaggctct caagggcatg ggtcggcgct ctcccttatg
     2101 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     2161 aaggaatggt gcatgtaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     2221 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     2281 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     2341 gatgcgtccg gcgtagagaa tccacaggac gggtgtggtc gccatgatcg cgtagtcgat
     2401 agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc
     2461 tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata
     2521 gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat
     2581 aaccaagcct atgcctacag
//
