LOCUS       sequence-353934-       11959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site 
            between Gag-NES and ABE. For production of virus like particles with
            base-editor RNP cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Banskota S, Raguram A, Suh S, Du SW, Davis JR, Choi EH, Wang X, 
            Nielsen SC, Newby GA, Randolph PB, Osborn MJ, Musunuru K, Palczewski
            K, Liu DR
  TITLE     Engineered virus-like particles for efficient in vivo delivery of 
            therapeutic proteins.
  JOURNAL   Cell. 2022 Jan 7. pii: S0092-8674(21)01484-7. doi: 
            10.1016/j.cell.2021.12.021.
  PUBMED    35021064
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Jan 7. pii: S0092-8674(21)01484-7. doi: 10.1016/j.cell.2021.12.021."
COMMENT     Sequence Label: pCMV-MMLVgag-3xNES-ABE7.10-NG
FEATURES             Location/Qualifiers
     source          1..11959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..420
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     350..372
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     388..404
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1633..1659
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1672..1698
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1711..1737
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1771..1794
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1828..1848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3037..7137
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7180..7200
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7206..7225)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7271..7326
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7325..7344)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7684..7700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7684..7700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7697..7719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7708..7724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7732..7738,7739..7756,7757..7762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7777..7798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7915..7932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8086..8674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8166..8185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8845..9636,9637..9705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9468..9487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9706..9810)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9836..10291)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9973..9994)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10185..10204
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10418..10440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10432..10449
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10433..10449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10459..10478
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10459..10477
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        10598..10977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10978..11181
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11132..11152
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11178..11202
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          11293..11865
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
       61 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
      121 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
      181 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
      241 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
      301 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
      361 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      421 aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa
      481 gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga
      541 gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg
      601 agacgggagc cccctgtggc cgactccact acctcgcagg cattccccct ccgcgcagga
      661 ggaaacggac agcttcaata ctggccgttc tcctcttctg acctttacaa ctggaaaaat
      721 aataaccctt ctttttctga agatccaggt aaactgacag ctctgatcga gtctgtcctc
      781 atcacccatc agcccacctg ggacgactgt cagcagctgt tggggactct gctgaccgga
      841 gaagaaaaac aacgggtgct cttagaggct agaaaggcgg tgcggggcga tgatgggcgc
      901 cccactcaac tgcccaatga agtcgatgcc gcttttcccc tcgagcgccc agactgggat
      961 tacaccaccc aggcaggtag gaaccaccta gtccactatc gccagttgct cctagcgggt
     1021 ctccaaaacg cgggcagaag ccccaccaat ttggccaagg taaaaggaat aacacaaggg
     1081 cccaatgagt ctccctcggc cttcctagag agacttaagg aagcctatcg caggtacact
     1141 ccttatgacc ctgaggaccc agggcaagaa actaatgtgt ctatgtcttt catttggcag
     1201 tctgccccag acattgggag aaagttagag aggttagaag atttaaaaaa caagacgctt
     1261 ggagatttgg ttagagaggc agaaaagatc tttaataaac gagaaacccc ggaagaaaga
     1321 gaggaacgta tcaggagaga aacagaggaa aaagaagaac gccgtaggac agaggatgag
     1381 cagaaagaga aagaaagaga tcgtaggaga catagagaga tgagcaagct attggccact
     1441 gtcgttagtg gacagaaaca ggatagacag ggaggagaac gaaggaggtc ccaactcgat
     1501 cgcgaccagt gtgcctactg caaagaaaag gggcactggg ctaaagattg tcccaagaaa
     1561 ccacgaggac ctcggggacc aagaccccag acctccctcc tgaccctaga tgactctggc
     1621 ggctcacttc aactgcctcc acttgaaaga ctgacactgg gatcattaca attacctcct
     1681 ttagaacgat taacactcgg ttcactacag cttccgcctc ttgagagatt gacattaacg
     1741 tccacgctgc taatggagaa ctcgtctgga gattacaaag acgatgacga taagaaacgg
     1801 acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaagtcgag
     1861 tttagccacg agtattggat gaggcacgca ctgaccctgg caaagcgagc atgggatgaa
     1921 agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg agagggatgg
     1981 aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc actgaggcag
     2041 ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt gacactggag
     2101 ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt ggtgttcgga
     2161 gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca ccaccccggc
     2221 atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc cgccctgctg
     2281 agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc acagagctcc
     2341 accgactctg gaggatctag cggaggatcc tctggaagcg agacaccagg cacaagcgag
     2401 tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc
     2461 cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag
     2521 gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga
     2581 gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc
     2641 ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc
     2701 gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg
     2761 aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat
     2821 caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat
     2881 ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac
     2941 tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca
     3001 acacctgaaa gcagcggggg cagcagcggg gggtcagaca agaagtacag catcggcctg
     3061 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     3121 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     3181 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3241 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3301 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3361 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3421 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3481 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3541 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3601 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     3661 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     3721 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     3781 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     3841 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     3901 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     3961 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     4021 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     4081 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     4141 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4201 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4261 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4321 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4381 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4441 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4501 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4561 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4621 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     4681 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     4741 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     4801 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     4861 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     4921 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     4981 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     5041 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     5101 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     5161 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5221 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5281 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5341 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5401 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5461 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5521 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5581 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     5641 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     5701 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     5761 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     5821 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     5881 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     5941 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     6001 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     6061 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     6121 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6181 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     6241 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     6301 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6361 atcaggccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6421 aagtacggcg gcttcgtcag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6481 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6541 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6601 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     6661 cggaagagaa tgctggcctc tgccagattc ctgcagaagg gaaacgaact ggccctgccc
     6721 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     6781 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     6841 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     6901 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     6961 atccacctgt ttaccctgac caatctggga gcccctaggg ccttcaagta ctttgacacc
     7021 accatcgacc ggaaggtgta caggagcacc aaagaggtgc tggacgccac cctgatccac
     7081 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct
     7141 ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc
     7201 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     7261 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     7321 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     7381 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     7441 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     7501 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     7561 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     7621 gtcatagctg tccctcttct cttatggaga tccctcgacg gatcggccgc aattcgtaac
     7681 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7741 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7801 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7861 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8521 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     9841 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     9901 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     9961 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
    10021 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
    10081 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
    10141 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
    10201 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
    10261 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
    10321 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    10381 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    10441 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt
    10501 ggcggccgct ctagagagct tggcccattg catacgttgt atccatatca taatatgtac
    10561 atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat
    10621 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    10681 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    10741 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    10801 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    10861 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    10921 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    10981 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    11041 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    11101 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    11161 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
    11221 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggtc gaccgatcct
    11281 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
    11341 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
    11401 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
    11461 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
    11521 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
    11581 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
    11641 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
    11701 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
    11761 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
    11821 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
    11881 ctggttgttg tgctgtctca tcattttggc aaagaattcc tcgagggccg gatccgaatt
    11941 cctgtatttg tctgaaaac
//
