LOCUS       sequence-35394-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: pCS2 Flag-JunB
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttcgccacca tggactacaa ggacgacgat gacaagtgca ctaaaatgga acagcccttc
       61 taccacgacg actcatacac agctacggga tacggccggg cccctggtgg cctctctcta
      121 cacgactaca aactcctgaa accgagcctg gcggtcaacc tggccgaccc ctaccggagt
      181 ctcaaagcgc ctggggctcg cggacccggc ccagagggcg gcggtggcgg cagctacttt
      241 tctggtcagg gctcggacac cggcgcgtct ctcaagctcg cctcttcgga gctggaacgc
      301 ctgattgtcc ccaacagcaa cggcgtgatc acgacgacgc ctacaccccc gggacagtac
      361 ttttaccccc gcgggggtgg cagcggtgga ggtgcagggg gcgcaggggg cggcgtcacc
      421 gaggagcagg agggcttcgc cgacggcttt gtcaaagccc tggacgatct gcacaagatg
      481 aaccacgtga caccccccaa cgtgtccctg ggcgctaccg gggggccccc ggctgggccc
      541 gggggcgtct acgccggccc ggagccacct cccgtttaca ccaacctcag cagctactcc
      601 ccagcctctg cgtcctcggg aggcgccggg gctgccgtcg ggaccgggag ctcgtacccg
      661 acgaccacca tcagctacct cccacacgcg ccgcccttcg ccggtggcca cccggcgcag
      721 ctgggcttgg gccgcggcgc ctccaccttc aaggaggaac cgcagaccgt gccggag
//
