LOCUS       sequence-353995-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(240..259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      685..1273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1174..1193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1400..1405,1406..1422,1423..1428)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1476..2600,1..66)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1828..1847)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
       61 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
      121 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta
      181 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc
      241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      361 aagctggtct catgacgcaa tcgctacaac ttgctcgggc gtcaggccgt gggcttgaca
      421 gagaactggt agaaggcgct gaacagtctc aagagcctgt ttgccacccc cattactagc
      481 aatcgctaca acttgatctg gggtgagtcc gtgggcttga cagagaactg gaaggaggcg
      541 ctgaaccgtc tcaagagcct gtttgccacc aatattagag cgagaccata tctggtcgag
      601 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
      661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1321 ttttgctcac atgttctttc ctgcgttatc cacagaatca ggggataacg caggaaagaa
     1381 catgaattaa ttctcatgtt tgacagctta tcatcgatta gctttaatgc ggtagtttat
     1441 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
     1501 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
     1561 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
     1621 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
     1681 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
     1741 tcatggcgac cacacccgtc ctgtggattc tctacgccgg acgcatcgtg gccggcatca
     1801 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     1861 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     1921 tggccggggg actgttgggc gccatctcct tacatgcacc attccttgcg gcggcggtgc
     1981 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     2041 gccgacccat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     2101 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2161 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     2221 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     2281 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     2341 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     2401 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     2461 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     2521 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     2581 tggcatggat tgtaggcgcc
//
