LOCUS       sequence-354000-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     365..384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(591..596,597..613,614..619)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             667..1857
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1019..1038)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2031..2050)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2032..2050)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2055..2071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2055..2071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2484..2608,1..464)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
       61 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      121 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      181 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      241 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      301 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      361 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      421 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      481 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      541 ccacagaatc aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt
      601 atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
      661 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
      721 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
      781 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
      841 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
      901 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt
      961 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1021 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1081 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1141 ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     1201 ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag agccttcaac
     1261 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     1321 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     1381 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     1441 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     1501 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     1561 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     1621 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     1681 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     1741 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     1801 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     1861 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     1921 tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt atgtcgagct
     1981 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc ccctatagtg
     2041 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2101 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc aaagctggtc
     2161 tcacctgatc tggggtgagt ccgtgggctt gacagagaac tggaaggagg cgctgaaccg
     2221 tctcaagagc ctgtttgcca ccaatattac tagcaatcgc tacaacttgc gctggggtta
     2281 gtccgtgggc ttgacagaga actggtagaa ggcgttggac ggtttcaaga gcctgtttgc
     2341 caccgtcatg actagcgatg gccaccacct gctccggggc gagaccatat ctggtcgagc
     2401 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2581 ctttttccga aggtaactgg cttcagca
//
