LOCUS       sequence-354011-       10063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM4 (with a C-terminal Flag tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM4-Flag
FEATURES             Location/Qualifiers
     source          1..10063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5231..5254
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5258..5282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5313..5901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5366..5386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5784..5795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5915..6414
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5950..5973)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6347..6366
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6435..7034
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6435..6454)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6931..6951
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7172..7405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7477..7598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7514..7533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7568..7587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7638..7773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7793..7812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7794..7812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7822..7839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7822..7838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7831..7853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7980..8435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8067..8086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8277..8298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8461..8565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8566..8634,8635..9426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8784..8803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9597..10063,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatgatgga
     3181 tgcatcaaaa gagctgcaag ttctccacat tgacttcttg aatcaggaca acgccgtttc
     3241 tcaccacaca tgggagttcc aaacgagcag tcctgtgttc cggcgaggac aggtgtttca
     3301 cctgcggctg gtgctgaacc agcccctaca atcctaccac caactgaaac tggaattcag
     3361 cacagggccg aatcctagca tcgccaaaca caccctggtg gtgctcgacc cgaggacgcc
     3421 ctcagaccac tacaactggc aggcaaccct tcaaaatgag tctggcaaag aggtcacagt
     3481 ggctgtcacc agttccccca atgccatcct gggcaagtac caactaaacg tgaaaactgg
     3541 aaaccacatc cttaagtctg aagaaaacat cctatacctt ctcttcaacc catggtgtaa
     3601 agaggacatg gttttcatgc ctgatgagga cgagcgcaaa gagtacatcc tcaatgacac
     3661 gggctgccat tacgtggggg ctgccagaag tatcaaatgc aaaccctgga actttggtca
     3721 gtttgagaaa aatgtcctgg actgctgcat ttccctgctg actgagagct ccctcaagcc
     3781 cacagatagg agggaccccg tgctggtgtg cagggccatg tgtgctatga tgagctttga
     3841 gaaaggccag ggcgtgctca ttgggaattg gactggggac tacgaaggtg gcacagcccc
     3901 atacaagtgg acaggcagtg ccccgatcct gcagcagtac tacaacacga agcaggctgt
     3961 gtgctttggc cagtgctggg tgtttgctgg gatcctgact acagtgctga gagcgttggg
     4021 catcccagca cgcagtgtga caggcttcga ttcagctcac gacacagaaa ggaacctcac
     4081 ggtggacacc tatgtgaatg agaatggcga gaaaatcacc agtatgaccc acgactctgt
     4141 ctggaatttc catgtgtgga cggatgcctg gatgaagcga ccggatctgc ccaagggcta
     4201 cgacggctgg caggctgtgg acgcaacgtc gcaggagcga agccagggtg tcttctgttg
     4261 tgggccatca ccactgaccg ccatccgcaa aggtgacatc tttattgtct atgacaccag
     4321 attcgtcttc tcagaagtga atggtgacag gctcatctgg ttggtgaaga tggtgaatgg
     4381 gcaggaggag ttacacgtaa tttcaatgga gaccacaagc atcgggaaaa acatcagcac
     4441 caaggcagtg ggccaagaca ggcggagaga tatcacctat gagtacaagt atccagaagg
     4501 ctcctctgag gagaggcagg tcatggatca tgccttcctc cttctcagtt ctgagaggga
     4561 gcacagacga cctgtaaaag agaactttct tcacatgtcg gtacaatcag atgatgtgct
     4621 gctgggaaac tctgttaatt tcaccgtgat tcttaaaagg aagaccgctg ccctacagaa
     4681 tgtcaacatc ttgggttcct ttgaactaca gttgtacact ggcaagaaga tggcaaaact
     4741 gtgtgacctc aataagacct cgcagatcca aggtcaagta tcagaagtga ctctgacctt
     4801 ggactccaag acctacatca acagcctggc tatattagat gatgagccag ttatcagagg
     4861 tttcatcatt gcggaaattg tggagtctaa ggaaatcatg gcctctgaag tattcacgtc
     4921 tttccagtac cctgagttct ctatagagtt gcctaacaca ggcagaattg gccagctact
     4981 tgtctgcaat tgtatcttca agaataccct ggccatccct ttgactgacg tcaagttctc
     5041 tttggaaagc ctgggcatct cctcactaca gacctctgac catgggacgg tgcagcctgg
     5101 tgagaccatc caatcccaaa taaaatgcac cccaataaaa actggaccca agaaatttat
     5161 cgtcaagtta agttccaaac aagtgaaaga gattaatgct cagaagattg ttctcatcac
     5221 caagggcggc gactacaaag acgatgacga caagtagacc cagctttctt gtacaaagtg
     5281 gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     5341 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5401 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5461 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5521 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5581 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5641 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5701 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5761 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5821 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5881 ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg
     5941 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6001 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6061 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6121 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6181 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6241 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6301 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6361 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6421 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6481 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6541 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6601 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6661 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     6721 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     6781 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     6841 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     6901 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6961 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7021 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     7081 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7141 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     7201 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7381 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7441 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7561 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7621 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7681 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7741 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     7801 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7861 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7921 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7981 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8041 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8101 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8161 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8221 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8281 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8341 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8401 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     8461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gag
//
