LOCUS       sequence-354067-       12069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac compatible plasmid expressing dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: NAX_CAGGS_CAS9_m4_KanR neo
FEATURES             Location/Qualifiers
     source          1..12069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          149..1165
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1089..1111
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1173..1192
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1262..1271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1268..1294,1295..1297,1298..3781,3782..3787,3788..3853,
                     3854..3856,3857..5371)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5384..5404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5429..5448)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5473..5521
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5753..5964
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5980..6774
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5980..6774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6034..6053)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6644..6663
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6778..6899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6815..6834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6869..6888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7662..7696
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8187..8203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8187..8203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8200..8222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8211..8227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8235..8241,8242..8259,8260..8265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8280..8301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8418..8435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8589..9177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8669..8688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9348..10139,10140..10208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9971..9990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10209..10313)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10339..10794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10476..10497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10688..10707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10921..10943
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10935..10952
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10936..10952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   11082..11144
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11565..11944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
       61 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      121 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
      181 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
      241 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
      301 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
      361 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
      421 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt ttgcgctccg
      481 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
      541 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
      601 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
      661 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
      721 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
      781 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
      841 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
      901 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
      961 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1021 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     1081 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1141 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     1201 tcattttggc aaagaatcta gataccctcg taaaggtcta gagctagcgg atcgaaccct
     1261 tgccaccatg gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg
     1321 ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac
     1381 cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac
     1441 ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg
     1501 gatctgctac ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt
     1561 ccataggctg gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat
     1621 ctttggcaat atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct
     1681 gaggaagaag cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct
     1741 ggcgcatatg atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa
     1801 cagcgatgtc gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga
     1861 gaacccgatc aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa
     1921 atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt
     1981 tggtaatctt atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct
     2041 ggccgaagat gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct
     2101 gctggcccag atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga
     2161 cgccattctg ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag
     2221 cgctagtatg atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct
     2281 tgtcagacag caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg
     2341 ctacgccgga tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc
     2401 catcttggaa aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct
     2461 gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga
     2521 actgcacgct atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga
     2581 aaagattgag aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg
     2641 aaattccaga ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt
     2701 cgaggaagtc gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt
     2761 tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt
     2821 cacagtttat aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc
     2881 attcctgtct ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa
     2941 agttaccgtg aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt
     3001 tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct
     3061 gaaaatcatt aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga
     3121 cattgtcctc acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac
     3181 ttacgctcat ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg
     3241 atgggggcgg ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac
     3301 aatcctggat tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca
     3361 tgatgactct ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga
     3421 cagtcttcac gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact
     3481 gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa
     3541 tatcgttatc gagatggccc gagagaacca aactacccag aagggacaga agaacagtag
     3601 ggaaaggatg aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga
     3661 acacccagtt gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa
     3721 cggcagggac atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt
     3781 ggctgctatc gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac
     3841 aagatccgat aaagctagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa
     3901 aatgaaaaat tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga
     3961 taatctgact aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa
     4021 aaggcagctt gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg
     4081 catgaacacc aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct
     4141 gaagtctaag ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat
     4201 caacaattac caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat
     4261 caaaaaatat cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt
     4321 taggaaaatg atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt
     4381 ttacagcaat attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg
     4441 gaagcgacca cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag
     4501 ggatttcgcg acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac
     4561 cgaagtacag accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa
     4621 gctgatcgca cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac
     4681 agtcgcttac agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa
     4741 aagcgtcaag gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc
     4801 catcgacttt ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct
     4861 tcccaagtac tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg
     4921 cgagctgcag aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct
     4981 ggccagccac tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt
     5041 cgtggaacaa cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa
     5101 aagagtgatc ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag
     5161 ggataagccc atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt
     5221 gggcgcgcct gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc
     5281 tacaaaggag gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac
     5341 aagaatcgac ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa
     5401 ggtgtgagtt taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     5461 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     5521 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5581 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5641 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgaaat
     5701 cggatccgcg gccacactga ttctagctgc agaggctccg gtgcccgtca gtgggcagag
     5761 cgcacatcgc ccacagcccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     5821 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5881 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5941 aacgggtttg ccgccagaac acaggatcct cgagccacca tgattgaaca agatggattg
     6001 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6061 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6121 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     6181 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6241 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6301 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6361 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6421 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6481 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     6541 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6601 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6661 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6721 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagttaac
     6781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6901 catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat ctggtctccc
     6961 ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc
     7021 ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt
     7081 ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg
     7141 gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     7201 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     7261 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     7321 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     7381 tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     7441 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     7501 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     7561 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     7621 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     7681 gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg
     7741 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     7801 ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga
     7861 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     7921 taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac
     7981 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     8041 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     8101 cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     8161 cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8281 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8341 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10321 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    10381 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    10441 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    10501 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    10561 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    10621 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    10681 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    10741 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
    10801 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10861 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10921 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg
    10981 aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga
    11041 tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata
    11101 ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt
    11161 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
    11221 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa
    11281 aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa
    11341 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
    11401 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
    11461 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
    11521 cctgcagaca cctgggggga tacggggaaa aggcctccac ggccgacatt gattattgac
    11581 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
    11641 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
    11701 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    11761 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
    11821 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
    11881 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
    11941 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
    12001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
    12061 ggggggggg
//
