LOCUS       sequence-354113-        9037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA EBFP2
FEATURES             Location/Qualifiers
     source          1..9037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          113..1129
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1053..1075
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1137..1156
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1157..1166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1163..1165,1166..1168,1169..1882)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    1906..2494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1959..1979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2377..2388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2701..3334
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3463..3479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3463..3479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3476..3498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3487..3503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3511..3517,3518..3535,3536..3541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3556..3577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3694..3711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3865..4453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3945..3964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4624..5415,5416..5484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5247..5266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5485..5589)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5637..5771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5674..5693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5728..5747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5939..6572
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6619..6744
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7241..7474
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7659..7703
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7966..8081
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8184..8432
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8184..8204
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8355..8374
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8454..8529
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8603..8906
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
       61 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      121 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      181 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      241 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      301 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
      361 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
      421 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
      481 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
      541 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
      601 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
      661 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
      721 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
      781 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
      841 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
      901 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
      961 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     1021 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1081 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1141 cgtgctggtt attgtggcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga
     1261 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tagtgaaccg catcgagctg aagggcgtcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acatctatat
     1621 catggccgtc aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacgtgga
     1681 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1741 cgtgctgctg cccgacagcc actacctgag cacccagtcc gtgctgagca aagaccccaa
     1801 cgagaagcgc gatcacatgg tcctgctgga gttccgcacc gccgccggga tcactctcgg
     1861 catggacgag ctgtacaagt aatgtacaag taaagcgtct ggaacaatca acctctggat
     1921 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     1981 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2041 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2101 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2161 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2221 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2281 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     2341 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2401 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2461 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     2521 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     2581 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     2641 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     2701 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     2761 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     2821 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     2881 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     2941 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     3001 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     3061 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     3121 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     3181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3301 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     3361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     3421 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     3481 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3541 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3601 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3661 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4501 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4561 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4621 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4681 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4741 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4801 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4861 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4921 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4981 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5041 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5101 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5161 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5221 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5281 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5341 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5401 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5461 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5521 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5581 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
     5641 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5701 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5761 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     5821 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     5881 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     5941 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     6001 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     6061 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     6121 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     6181 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     6241 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     6301 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     6361 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     6421 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6481 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6541 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6601 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6661 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6721 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6781 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6841 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6901 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6961 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7021 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7081 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7141 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7201 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7261 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7321 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7381 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7441 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7501 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7561 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7621 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7681 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7741 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7801 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7861 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7921 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     7981 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8041 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8101 agcagagatc cagtttatca ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     8161 ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
     8221 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     8281 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     8341 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     8401 ctttatatat cttgtggaaa ggacgaaaca ccggagacga ttaatgcgtc tcggttttag
     8461 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     8521 agtcggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattcg tcattagttc
     8581 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8641 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     8701 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     8761 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     8821 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     8881 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     8941 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     9001 cagcgatggg ggcggggggg ggggcgcgcg ccaggcg
//
