LOCUS       sequence-354119-        9251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA iRFP713
FEATURES             Location/Qualifiers
     source          1..9251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          110..1126
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1050..1072
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1134..1153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1154..1163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1160..2110
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    2134..2722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2187..2207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2605..2616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2929..3562
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3691..3707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3691..3707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3704..3726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3715..3731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3739..3745,3746..3763,3764..3769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3784..3805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3922..3939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4852..5643,5644..5712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5475..5494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5713..5817)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5865..5999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5902..5921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5956..5975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6167..6800
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6847..6972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7469..7702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7887..7931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8194..8309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8412..8660
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8412..8432
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8583..8602
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8682..8757
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8831..9134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      361 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      421 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      481 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      541 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      601 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      661 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      721 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
      781 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      841 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      901 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      961 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1021 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1081 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1141 gctggttatt gtggccacca tggcggaagg atccgtcgcc aggcagcctg acctcttgac
     1201 ctgcgacgat gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc
     1261 cctcgccgcc gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact
     1321 ggcgatcggc gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg aaacgcacaa
     1381 ccgtctgacg atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg
     1441 cttcacgatg cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat
     1501 cttcctcgag ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg
     1561 caccaacagc gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc
     1621 cgcggcgcaa gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc
     1681 ctccgacttc agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact
     1741 aggcctgcac tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa
     1801 cccggtacgg atcattcccg atatcaatta tcggccggtg ccggtcaccc cagacctcaa
     1861 tccggtcacc gggcggccga ttgatcttag cttcgccatc ctgcgcagcg tatcgcccgt
     1921 ccatctggag ttcatgcgca acataggcat gcacggcacg atgtcgatct cgattttgcg
     1981 cggcgagcga ctgtggggat tgatcgtttg ccatcaccga acgccgtact acgtcgatct
     2041 cgatggccgc caagcctgcg agctagtcgc ccaggttctg gcctggcaga tcggcgtgat
     2101 ggaagagtga tgtacaagta aagcgtctgg aacaatcaac ctctggatta caaaatttgt
     2161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2521 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     2581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2701 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     2761 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     2821 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     2881 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     2941 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     3001 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     3061 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     3121 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     3181 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     3241 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     3301 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     3361 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3541 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3601 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc
     3661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5821 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     6001 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     6061 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     6121 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     6181 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6241 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6301 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     6361 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     6421 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     6481 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     6541 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     6601 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6781 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6841 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6901 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6961 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7021 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7081 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7141 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7201 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7261 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     7321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8161 ttcgattagt gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg
     8221 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     8281 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     8341 gtttatcatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
     8401 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     8461 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     8521 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     8581 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     8641 tgtggaaagg acgaaacacc ggagacgatt aatgcgtctc ggttttagag ctagaaatag
     8701 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     8761 ttttgaattc tcgacctcga gacaaatggc agtattcgtc attagttcat agcccatata
     8821 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     8881 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     8941 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     9001 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     9061 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     9121 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     9181 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     9241 cggggggggg g
//
