LOCUS       sequence-354129-        9274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA iRFP713_TtoC and hairpin ext...
FEATURES             Location/Qualifiers
     source          1..9274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1166..1175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1172..2122
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    2146..2734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2199..2219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2617..2628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2941..3574
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3703..3719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3703..3719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3716..3738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3727..3743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3751..3757,3758..3775,3776..3781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3796..3817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3934..3951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4105..4693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4185..4204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4864..5655,5656..5724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5487..5506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5725..5829)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5877..6011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5914..5933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5968..5987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6179..6812
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6859..6984
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7481..7714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7899..7943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8206..8321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8424..8672
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8424..8444
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8595..8614
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        8853..9156
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtggccac catggcggaa ggatccgtcg ccaggcagcc
     1201 tgacctcttg acctgcgacg atgagccgat ccatatcccc ggtgccatcc aaccgcatgg
     1261 actgctgctc gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca accttcccga
     1321 actcaccgga ctggcgatcg gcgccctgat cggccgctct gcggccgatg tcttcgactc
     1381 ggaaacgcac aaccgtctga cgatcgcctt ggccgagccc ggggcggccg tcggagcacc
     1441 gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc atcggctcct ggcatcgcca
     1501 tgatcagctc atcttcctcg agctcgagcc tccccagcgg gacgtcgccg agccgcaggc
     1561 gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa ccttggaaag
     1621 cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc gggtgatgat
     1681 ctatcgcttc gcctccgact tcagcggcga agtgatcgca gaggatcggt gcgccgaggt
     1741 cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg ccggcgcagg cccgtcggct
     1801 ctataccatc aacccggtac ggatcattcc cgatatcaat tatcggccgg tgccggtcac
     1861 cccagacctc aatccggtca ccgggcggcc gattgatctt agcttcgcca tcctgcgcag
     1921 cgtatcgccc gtccatctgg agttcatgcg caacataggc atgcacggca cgatgtcgat
     1981 ctcgattttg cgcggcgagc gactgtgggg attgatcgtt tgccatcacc gaacgccgta
     2041 ctacgtcgat ctcgatggcc gccaagcctg cgagctagtc gcccaggttc tggcctggca
     2101 gatcggcgtg atggaagagt gatgtacaag taaagcgtct ggaacaatca acctctggat
     2161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     2581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2701 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     2761 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     2821 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     2881 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     2941 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     3001 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     3061 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     3121 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     3181 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     3241 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     3301 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     3361 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     3421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3541 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     3601 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     3661 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     3721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
     5881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6001 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     6061 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     6121 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     6181 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     6241 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     6301 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     6361 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     6421 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     6481 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     6541 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     6601 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     6661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6781 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6841 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6901 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6961 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7021 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7081 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7141 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7201 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7261 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7321 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     8221 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8281 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8341 agcagagatc cagtttatca ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     8401 ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
     8461 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     8521 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     8581 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     8641 ctttatatat cttgtggaaa ggacgaaaca ccggagacga ttaatgcgtc tcggtttaag
     8701 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     8761 agtggcaccg agtcggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattcg
     8821 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     8881 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     8941 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9001 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9061 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     9121 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     9181 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     9241 ttattttgtg cagcgatggg ggcggggggg gggg
//
