LOCUS       sequence-354137-        9359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA iRFP713_zhang2.0
FEATURES             Location/Qualifiers
     source          1..9359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          134..1150
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1074..1096
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1158..1177
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1178..1187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1184..2134
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    2158..2746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2211..2231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2629..2640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2953..3586
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3715..3731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3715..3731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3728..3750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3739..3755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3763..3769,3770..3787,3788..3793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3808..3829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3946..3963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4117..4705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4197..4216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4876..5667,5668..5736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5499..5518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5737..5841)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5889..6023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5926..5945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5980..5999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6191..6824
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6871..6996
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7493..7726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7911..7955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8218..8333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8436..8684
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8436..8456
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8607..8626
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8723..8741
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8793..8811
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        8915..9218
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9220..9359,1..133)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
       61 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      121 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      181 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
      241 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
      301 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
      361 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
      421 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
      481 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
      541 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
      601 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
      661 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
      721 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
      781 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
      841 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
      901 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
      961 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1021 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1081 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     1141 tttcctacag ctcctgggca acgtgctggt tattgtggcc accatggcgg aaggatccgt
     1201 cgccaggcag cctgacctct tgacctgcga cgatgagccg atccatatcc ccggtgccat
     1261 ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg acgatcgttg ccggcagcga
     1321 caaccttccc gaactcaccg gactggcgat cggcgccctg atcggccgct ctgcggccga
     1381 tgtcttcgac tcggaaacgc acaaccgtct gacgatcgcc ttggccgagc ccggggcggc
     1441 cgtcggagca ccgatcactg tcggcttcac gatgcgaaag gacgcaggct tcatcggctc
     1501 ctggcatcgc catgatcagc tcatcttcct cgagctcgag cctccccagc gggacgtcgc
     1561 cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc cgccgcctgc aggccgccga
     1621 aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg cggaagatta ccggcttcga
     1681 tcgggtgatg atctatcgct tcgcctccga cttcagcggc gaagtgatcg cagaggatcg
     1741 gtgcgccgag gtcgagtcaa aactaggcct gcactatcct gcctcaaccg tgccggcgca
     1801 ggcccgtcgg ctctatacca tcaacccggt acggatcatt cccgatatca attatcggcc
     1861 ggtgccggtc accccagacc tcaatccggt caccgggcgg ccgattgatc ttagcttcgc
     1921 catcctgcgc agcgtatcgc ccgtccatct ggagttcatg cgcaacatag gcatgcacgg
     1981 cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg ggattgatcg tttgccatca
     2041 ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc tgcgagctag tcgcccaggt
     2101 tctggcctgg cagatcggcg tgatggaaga gtgatgtaca agtaaagcgt ctggaacaat
     2161 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2221 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2281 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2341 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2401 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2461 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2521 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     2581 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2641 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2701 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     2761 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     2821 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     2881 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     2941 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     3001 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     3061 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     3121 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     3181 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     3241 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     3301 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     3361 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     3421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3601 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     3661 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     3721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4861 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4921 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4981 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5041 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5101 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5161 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5221 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5281 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5341 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5401 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5461 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5521 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5581 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5641 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5701 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5761 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5821 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     5881 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6001 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     6061 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     6121 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     6181 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     6241 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6301 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6361 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     6421 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     6481 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     6541 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6601 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6661 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6721 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6781 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6841 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6901 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6961 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7021 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7081 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7141 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7201 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7261 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7321 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7381 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7441 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7501 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7561 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7621 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7681 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7741 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7801 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7861 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7921 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7981 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8041 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8101 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8161 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt
     8221 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8281 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     8341 tattacaggg acagcagaga tccagtttat cattagtgaa cggatctcga cggtatcgat
     8401 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt
     8461 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     8521 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     8581 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     8641 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac gattaatgcg
     8701 tctcggtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
     8761 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
     8821 caagtggcac cgagtcggtg ctttttttga attctcgacc tcgagacaaa tggcagtatt
     8881 cgtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9061 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     9121 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     9181 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     9241 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     9301 aattattttg tgcagcgatg ggggcggggg gggggggcgc gcgccaggcg gggcggggc
//
