LOCUS       sequence-354138-        9086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA EYFP_zhang2.0
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          113..1129
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1053..1075
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1137..1156
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1157..1166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1163..1165,1166..1168,1169..1882)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1469..1488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1816..1837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1895..2483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1948..1968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2366..2377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2690..3323
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3452..3468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3452..3468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3465..3487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3476..3492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3500..3506,3507..3524,3525..3530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3545..3566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3683..3700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3854..4442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3934..3953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4613..5404,5405..5473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5236..5255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5474..5578)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5626..5760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5663..5682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5717..5736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5928..6561
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6608..6733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7230..7463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7648..7692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7955..8070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8173..8421
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8173..8193
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8344..8363
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8460..8478
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8530..8548
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        8652..8955
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
       61 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      121 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      181 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      241 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      301 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
      361 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
      421 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
      481 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
      541 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
      601 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
      661 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
      721 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
      781 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
      841 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
      901 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
      961 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     1021 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1081 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1141 cgtgctggtt attgtggcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1261 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1621 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1681 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1741 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     1801 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1861 catggacgag ctgtacaagt aaagcgtctg gaacaatcaa cctctggatt acaaaatttg
     1921 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2281 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     2341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2401 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2461 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     2521 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     2581 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     2641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     2701 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     2761 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     2821 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     2881 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     2941 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     3001 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     3061 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     3121 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     3181 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3241 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3301 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     3361 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     3421 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3481 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3541 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3601 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3661 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3721 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3781 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3841 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3901 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3961 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4021 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4081 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4141 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4201 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4261 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4321 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4381 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5581 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
     5641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     5761 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     5821 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     5881 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     5941 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6001 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     6061 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     6121 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     6181 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     6241 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     6301 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     6361 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6541 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6601 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6661 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6721 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6781 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6841 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6901 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     6961 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7021 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     7081 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     7141 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     7201 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     7261 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     7321 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     7381 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     7441 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     7501 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     7561 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     7621 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     7681 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     7741 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     7801 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     7861 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     7921 attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg ggattggggg
     7981 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8041 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8101 agtttatcat tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgagcggcc
     8161 gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     8221 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     8281 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     8341 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     8401 ttgtggaaag gacgaaacac cggagacgat taatgcgtct cggttttaga gctaggccaa
     8461 catgaggatc acccatgtct gcagggccta gcaagttaaa ataaggctag tccgttatca
     8521 acttggccaa catgaggatc acccatgtct gcagggccaa gtggcaccga gtcggtgctt
     8581 tttttgaatt ctcgacctcg agacaaatgg cagtattcgt cattagttca tagcccatat
     8641 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     8701 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     8761 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     8821 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     8881 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     8941 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     9001 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     9061 gcgggggggg ggggcgcgcg ccaggc
//
