LOCUS       sequence-35417-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, 
            Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li 
            X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T,
            Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, 
            Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, 
            Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu
            B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L,
            Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar
            Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley
            DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu 
            VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, 
            Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, 
            Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, 
            Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, 
            Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D
  TITLE     Community-Wide Assessment of Protein-Interface Modeling Suggests 
            Improvements to Design Methodology.
  JOURNAL   J Mol Biol. 2011 Sep 29.
  PUBMED    22001016
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Sep 29."
COMMENT     Sequence Label: pETCON-E49/design_82
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..307
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             308..334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             647..655
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tacattttca attaagatgc agttacttcg ctgtttttca atattttctg ttattgcttc
       61 agttttagca caggaactga caactatatg cgagcaaatc ccctcaccaa ctttagaatc
      121 gacgccgtac tctttgtcaa cgactactat tttggccaac gggaaggcaa tgcaaggagt
      181 ttttgaatat tacaaatcag taacgtttgt cagtaattgc ggttctcacc cctcaacaac
      241 tagcaaaggc agccccataa acacacagta tgtttttaag gacaatagct cgacgattga
      301 aggtagatac ccatacgacg ttccagacta cgctctgcag gctagtggtg gaggaggctc
      361 tggtggaggc ggtagcggag gcggagggtc ggctagccat atgatgaata ctagaacaat
      421 ttatttggct ggtggttgtt tttggggttt agaagcatac ttccaaagaa ttgatggtgt
      481 tgttgatgct gtttctggtt atgcaaacgg taacactaag aacccatctt atgaagatgt
      541 ttcatacaga catacaggtc atgctgaaac agttaaagtt acctatgatg ctgataagtt
      601 gtcattgaac gatatcttgc aatacttttt cagagttgtt gatccaacct ccttaaataa
      661 gcagggtaac gataccggta ctcaatatag aagtggtgtt tattacacag atcctgctga
      721 agcaagagtt atcgctttgg cattgatgtt cgaacaatct aagtaccaat tgccattggt
      781 tgttgaaaac ga
//
