LOCUS       sequence-354241-        9422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of a gRNA-tRNA array for macsGESTALT 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, 
            Shendure J, McKenna A, Lengner CJ
  TITLE     Single-cell lineage tracing of metastatic cancer reveals selection 
            of hybrid EMT states.
  JOURNAL   Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 
            10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10.
  PUBMED    34115987
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2021.05.005"; journalName: "Cancer Cell"; date: 
            "2021-08-9- 9"; volume: "39"; issue: "8"; pages: "1150-1162.e9"
COMMENT     Sequence Label: pUltra-U6-gRNAs1-5
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2261
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2013..2033
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2184..2203
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2283..2358
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2457..2532
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2631..2706
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2805..2880
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2979..3054
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3118..4329
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4166..4185
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4362..4371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4368..4370,4371..4373,4374..5084)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4368..4370,4371..4373,4374..5084)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4413..4434)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4674..4693)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5021..5042
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5094..5150
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5172..5225
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5269..5857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5322..5342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5740..5751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5948..6181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6253..6374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6290..6309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6344..6363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6414..6549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6569..6588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6570..6588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6598..6615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6598..6614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6607..6629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6756..7211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6843..6862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7053..7074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7237..7341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7342..7410,7411..8202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7560..7579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8373..8961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8862..8881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9115..9132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9249..9270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9285..9290,9291..9308,9309..9315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9323..9339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9328..9350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9347..9363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9347..9363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9382..9402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9384..9402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatgg cgcgccggta ccgagggcct atttcccatg attccttcat
     2041 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     2101 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2161 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2221 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgttagagcc tggctggtct
     2281 ccgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     2341 gtggcaccga gtcggtgcta acaaagcacc agtggtctag tggtagaata gtaccctgcc
     2401 acggtacaga cccgggttcg attcccggct ggtgcactta atgagcaatt cctctcgttt
     2461 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     2521 ccgagtcggt gctaacaaag caccagtggt ctagtggtag aatagtaccc tgccacggta
     2581 cagacccggg ttcgattccc ggctggtgca agtattgcta tctaggataa gttttagagc
     2641 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     2701 cggtgctaac aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc
     2761 cgggttcgat tcccggctgg tgcaggtcct gcaaggatta ttatgtttta gagctagaaa
     2821 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     2881 taacaaagca ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt
     2941 cgattcccgg ctggtgcatt ataatcaact gcctcttcgt tttagagcta gaaatagcaa
     3001 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     3061 gcctacctgg agcctgagag ttgttcgata tcattaaccc gtgtcggctc cagatctggc
     3121 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     3181 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     3241 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     3301 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     3361 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     3421 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     3481 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
     3541 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
     3601 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
     3661 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     3721 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     3781 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     3841 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     3901 ggcggtgccg ttgggcagtg cacccgtacc tttgggrgcg cgcgccctcg tcgtgtcgtg
     3961 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     4021 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     4081 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     4141 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     4201 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     4261 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     4321 ttgttagacg aagcttgggc tgcaggtccg atccaccggt cgccaccatg gtgagcaagg
     4381 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4441 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4501 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4561 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4621 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4681 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4741 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4801 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4861 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4921 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4981 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5041 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggatct ggagcaacaa
     5101 acttctcact actcaaacaa gcaggtgacg tggaggagaa tcccgggcct tctagaggat
     5161 ccggctccgg cgagggcagg ggaagtctac taacatgcgg ggacgtggag gaaaatcccg
     5221 gcccagctag cgtgtgatca gaattcgttc cggagtcgtc gactcgacaa tcaacctctg
     5281 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5341 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5401 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5461 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5521 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5581 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5641 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5701 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5761 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcatcttcg ccttcgccct
     5821 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc gagctcggta
     5881 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5941 gggggactgg aagggctacg taactcccaa cgaagacaag atctgctttt tgcttgtact
     6001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6181 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6241 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6361 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6541 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6601 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6661 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6721 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6841 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6901 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6961 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7021 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7081 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7141 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7201 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7261 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7321 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7381 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7441 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7501 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7561 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7621 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7681 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7741 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7801 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7861 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7921 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7981 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8041 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8101 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8161 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8881 tatagtcctg tcgggtttcg ccacctctca cttgagcgtc gatttttgtg atgctcgtca
     8941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9061 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9121 tcagtgagcg aggaagsgga agagcsccma atacgcaaac cgcctctccc cgcgcgttgg
     9181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9301 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9361 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     9421 ca
//
