LOCUS       sequence-354255-        8438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It encodes an mVenus fused to a mutant p27K-, which lacks binding 
            affinity to Cdk. This reporter helps to identify quiescent 
            disseminated tumor cells (in G0 phase of cell cycle)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Correia AL, Guimaraes JC, Auf der Maur P, De Silva D, Trefny MP, 
            Okamoto R, Bruno S, Schmidt A, Mertz K, Volkmann K, Terracciano L, 
            Zippelius A, Vetter M, Kurzeder C, Weber WP, Bentires-Alj M
  TITLE     Hepatic stellate cells suppress NK cell-sustained breast cancer 
            dormancy.
  JOURNAL   Nature. 2021 Jun;594(7864):566-571. doi: 10.1038/s41586-021-03614-z.
            Epub 2021 Jun 2.
  PUBMED    34079127
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03614-z"; journalName: "Nature"; date: 
            "2021-06"; volume: "594"; issue: "7864"; pages: "566-571"
COMMENT     Sequence Label: pCDH-EF1-mVenus-p27K−
FEATURES             Location/Qualifiers
     source          1..8438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..88
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     80..97
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     81..97
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        112..338
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             339..519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    566..691
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1184..1417
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1602..1646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1913..2029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2072..2283
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2296..2564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(2592..2594,2595..2597,2598..3308)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2637..2658)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2898..2917)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3245..3266
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3951..4004
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4011..4610
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4011..4030)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4507..4527
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4623..5211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4676..4696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5094..5105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5285..5518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5590..5711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5681..5700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5751..5886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5919..5935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5919..5935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5932..5954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5943..5959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5967..5973,5974..5991,5992..5997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6012..6033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6150..6167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6321..6909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7080..7871,7872..7940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7703..7722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7941..8045)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8113..8131
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8169..8191)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8291..8310
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
       61 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgacgc gtgtagtctt
      121 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
      181 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
      241 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
      301 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
      361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      541 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      601 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      661 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      721 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      781 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      841 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
      901 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
      961 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1021 acagcaagcg gccactgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1081 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1141 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1201 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1261 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1321 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1381 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1441 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1501 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1561 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1621 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1681 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1741 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1801 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1861 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     1921 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1981 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgatactag
     2041 tggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2101 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     2161 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2221 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2281 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     2341 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     2401 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     2461 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     2521 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     2581 cctactctag aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2641 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2701 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     2761 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     2821 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3001 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     3061 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     3121 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3181 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
     3241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3301 tgtacaaggg atatccatca cactggcggc cgctcgaggc caccatgtca aacgtgagag
     3361 tgtctaacgg gagcccgagc ctggagcgga tggacgccag acaagcggag caccccaagc
     3421 cttccgcctg cagaaatctc ttcggcccgg tcaatcatga agaactaacc cgggacttgg
     3481 agaagcactg ccgggatatg gaagaagcga gtcagcgcaa gtggaatgcc gacgcccaga
     3541 atcataagcc cctggagggc agatacgagt ggcaggaggt ggagaggggc agcttgcccg
     3601 agttctacta caggcccccg cgccccccca agagcgcctg caaggtgctg gcgcaggaga
     3661 gccaggatgt cagcgggagc cgccaggcgg tgcctttaat tgggtctcag gcaaactctg
     3721 aggaccggca tttggtggac caaatgcctg actcgtcaga caatccggct gggttagcgg
     3781 agcagtgtcc agggatgagg aagcgacctg ctgcagaaga ttcttcttcg caaaacaaaa
     3841 gggccaacag aacagaagaa aatgtttcag acggttcccc gaacgctggc actgtggagc
     3901 agacgcccaa gaagcccggc cttcgacgcc agacgggatc cgcggccgct gagggcagag
     3961 gaagtcttct aacatgcggt gacgtggagg agaatcccgg cccttccggg atgaccgagt
     4021 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4081 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4141 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4201 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4261 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4321 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4381 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4441 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4501 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4561 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga atctaggtcg
     4621 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4741 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4801 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4861 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4921 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4981 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5161 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct
     5221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5281 ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
     5341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5521 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5581 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5881 gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga
     5941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6721 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6961 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7021 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8041 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8101 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8161 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8221 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     8281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     8341 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     8401 aactgttggg aagggcgatc ggtgcgggcc tcttcgct
//
