LOCUS       sequence-354259-        8394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral reverse tetracycline-controlled transactivator 3 
            (rtTA3) expression vector, CMV promoter, Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8394)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV rtTA3 Blast (w756-1)
FEATURES             Location/Qualifiers
     source          1..8394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2353..2656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2657..2860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2811..2831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2857..2881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2965..2989
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2992..3699
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(3698..3722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3753..4341
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3806..3826)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4224..4235)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4392..4412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4397..4726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4577..4712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4774..4821
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4840..5238
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5325..5558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5630..5751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5667..5686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5721..5740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5791..5926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5946..5965)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5947..5965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5975..5992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5975..5991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5984..6006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6133..6588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6220..6239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6430..6451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6614..6718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6719..6787,6788..7579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6937..6956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7750..8338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8239..8258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     2341 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2401 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2461 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2521 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2581 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2641 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2701 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2761 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2821 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagtccag
     2941 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagttggcac catgtctaga
     3001 ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa
     3061 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3121 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3181 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3241 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3301 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3361 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3421 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3481 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     3541 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3601 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     3661 gcccttgacg attttgacct tgacatgctc cccgggtaat caactttctt gtacaaagtg
     3721 gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     3781 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3841 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3901 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3961 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4021 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4081 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4141 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4201 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4261 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4321 ccctttgggc cgcctccccg cctggaattc tgcagatatc cggttagtaa tgagtttgga
     4381 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4441 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4561 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4741 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     4801 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa
     4861 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     4921 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     4981 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     5041 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     5101 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     5161 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     5221 tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     5341 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5581 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5761 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5941 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6121 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6301 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctca
//
