LOCUS       sequence-354261-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  13S-S Candidatus "Scalindua brodae" Twin- Strep SUMO tagged RAMP 
            (codon-optimization) enriched with CRISPR array spacer 1 (five 
            times).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   van Beljouw SPB, Haagsma AC, Rodriguez-Molina A, van den Berg DF, 
            Vink JNA, Brouns SJJ
  TITLE     The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with 
            a caspase-like peptidase.
  JOURNAL   Science. 2021 Sep 17;373(6561):1349-1353. doi: 
            10.1126/science.abk2718. Epub 2021 Aug 26.
  PUBMED    34446442
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abk2718"; journalName: "Science"; date: 
            "2021-09-17- 17"; volume: "373"; issue: "6561"; pages: "1349-1353"
COMMENT     Sequence Label: pTU422
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..183
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    301..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             882..926
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      978..1025
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1141..1932)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1933..2024)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2072..2810)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(3019..4101)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4063..4082
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4102..4179)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4225..4243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4248..4271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4324..4346
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4372..4395
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4432..4455
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4474..4767
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             4774..4794
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attggaagtg gataacggat ccgcgatcgc ggcgcgccac ctggtggccg gccggtacca
       61 cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      121 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      181 ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
      241 ccggtaaata atcgtattgt acacggccgc ataatcgaaa ttaatacgac tcactatagg
      301 ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat aagaaggaga
      361 tatacatatg tacaaaatgg ccccttctcg ccatatacgt aacctcagag ttgttggagg
      421 gttatgaaac aagagaagga cttaatgtca cggtacccaa ttttctgccc cggactccac
      481 ggctgttact agaggttatg aaacaagaga aggacttaat gtcacggtac ccaattttct
      541 gccccggact ccacggctgt tactagaggt tatgaaacaa gagaaggact taatgtcacg
      601 gtacccaatt ttctgccccg gactccacgg ctgttactag aggttatgaa acaagagaag
      661 gacttaatgt cacggtaccc aattttctgc cccggactcc acggctgtta ctagaggtta
      721 tgaaacaaga gaaggactta atgtcacggt acccaatttt ctgccccgga ctccacggct
      781 gttactagag gttatgaaac aagagaagga cttaatgtca cggtaccaat tggatatcgg
      841 ccggccacgc gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc gctgctgcga
      901 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg
      961 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     1021 ttttgctgaa acctcccagc aatagacata agcggctatt taacgaccct gccctgaacc
     1081 gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca
     1141 ttatttgccg actaccttgg tgatctcgcc tttcacgtaa tggacaaatt cttccaactg
     1201 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1261 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1321 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1381 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1441 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1501 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1561 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     1621 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     1681 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     1741 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     1801 cggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     1861 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     1921 cgcttccctc atactcttcc tttttcatta ttattgaagc atttatcagg gttattgtct
     1981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg
     2041 tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac
     2101 agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg
     2161 gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt
     2221 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     2281 gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac
     2341 cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct
     2401 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     2461 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     2521 ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg
     2581 cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta
     2641 cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag
     2701 ttccccaact gncttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag
     2761 ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg
     2821 aaccgctctt atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
     2881 acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact
     2941 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     3001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     3121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     3181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     3241 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     3301 gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     3361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     3421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     3481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     3541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     3601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     3661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     3721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     3781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     3841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     3901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     3961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     4021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     4081 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     4141 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     4201 tatgcgactc ctgcattagg aaattaatac gactcactat agggagagaa ttgtgagcgg
     4261 ataacaattc ccctgtagat taattaagcg gccgccctgc aggactcgag ttctagaaat
     4321 aattttgttt aactttaaga aggagatata catatgaaat cttcttccgc gtggtctcac
     4381 cctcagtttg aaaaaggcgg cggttctggc ggcggctccg gcggtggtgc ttggtcccac
     4441 ccgcagtttg aaaaaggttc ttctatggct agcatgtcgg actcagaagt caatcaagaa
     4501 gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt aaaggtgtcc
     4561 gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag aaggctgatg
     4621 gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt gtacgacggt
     4681 attagaattc aagctgatca gacccctgaa gatttggaca tggaggataa cgatattatt
     4741 gaggctcaca gagaacagat tggtgggatc gaggaaaacc tgtacttcca atccaatgca
     4801 atgaaaagca acgacatgaa cattaccgtg gaactgacct tttttgaacc gtatcgtctg
     4861 gttgaatggt ttgattggga tgcacgtaaa aaaagccata gcgcaatgcg tggtcaggca
     4921 tttgcacagt ggacctggaa aggtaaaggt cgtaccgcag gtaaaagctt tattaccggt
     4981 acactggttc gtagcgcagt tattaaagca gttgaagaac tgctgagcct gaataatggt
     5041 aaatgggaag gtgttccgtg ttgcaatggt agctttcaga ccgatgaaag caaaggtaaa
     5101 aaaccgagct ttctgcgtaa acgtcatacc ctgcagtggc aggcaaataa caaaaacatt
     5161 tgcgataaag aagaggcctg tccgttttgt attctgctgg gtcgttttga taatgccggt
     5221 aaagtgcatg aacgcaacaa agattatgat atccacttca gcaacttcga cctggatcac
     5281 aaacaagaaa aaaatgatct gcgcctggtt gatattgcaa gcggtcgtat tctgaatcgt
     5341 gttgattttg ataccggcaa agccaaagat tactttcgta cctgggaagc agattatgaa
     5401 acctatggca cctataccgg tcgcattacc ctgcgtaatg aacatgcaaa aaaactgctg
     5461 ctggcaagcc tgggttttgt tgataaactg tgtggtgcac tgtgtcgtat tgaggttatc
     5521 aaaaaaagcg aaagtccgct gccgagcgat accaaagaac agagctatac aaaagatgat
     5581 accgttgaag ttctgagcga agatcataat gatgaactgc gcaaacaggc cgaagttatt
     5641 gttgaagcat ttaagcagaa cgataaactg gaaaaaattc gcattctggc agatgcaatt
     5701 cgtaccctgc gcctgcatgg tgaaggtgtg attgaaaaag atgagctgcc ggatggtaaa
     5761 gaagaacgcg ataaaggtca tcatctgtgg gatattaaag ttcagggcac cgcactgcgt
     5821 accaaactga aagaactgtg gcagagcaat aaagatattg gctggcgcaa atttaccgaa
     5881 atgctgggta gcaatctgta cctgatctat aagaaagaaa ccggtggtgt tagcacccgt
     5941 tttcgcatcc tgggtgatac cgagtattat agcaaagcac atgatagcga aggtagcgac
     6001 ctgtttattc cggttacacc gcctgaaggt attgaaacca aagaatggat tattgtgggt
     6061 cgcctgaaag cagcaacccc gttttatttc ggtgttcagc agccgagtga tagcattccg
     6121 ggtaaagaga aaaaatcaga agatagcctg gtcatcaatg aacacaccag ctttaacatc
     6181 ctgctggata aagaaaatcg ttatcgtatt ccgcgtagtg cactgcgtgg tgccctgcgt
     6241 cgcgatctgc gtaccgcatt tggtagcggt tgtaatgtta gcttaggtgg tcagattctg
     6301 tgcaattgta aagtgtgtat tgaaatgcgt cgcatcacac tgaaagatag cgttagcgat
     6361 ttttcagaac ctccggaaat tcgctatcgc attgcaaaaa atccgggtac agcaaccgtg
     6421 gaagatggta gtctgtttga tattgaagtt ggtccggaag gcctgacctt tccgtttgtt
     6481 ctgcgttatc gtggtcataa atttccagaa cagctgagca gcgttattcg ttattgggaa
     6541 gaaaatgatg gcaaaaatgg tatggcatgg ttaggtggcc tggatagcac cggtaaaggc
     6601 cgttttgccc tgaaagacat taaaatcttt gagtgggatc tgaaccagaa aatcaacgaa
     6661 tatatcaaag aacgcggtat gcgtggcaaa gaaaaagaat tactggaaat gggtgaaagc
     6721 agtctgcctg atggtctgat tccgtataaa ttctttgaag aacgtgaatg cctgtttccg
     6781 tacaaagaaa acctgaaacc gcagtggtca gaagttcagt ataccattga agtgggttca
     6841 ccgctgctga ccgcagatac cattagcgca ctgaccgaac cgggtaatcg tgatgcaatt
     6901 gcctacaaaa aacgcgtgta taacgatggc aataatgcca ttgaaccgga accgcgtttt
     6961 gcagttaaaa gtgaaaccca tcgtggtatt tttcgcaccg cagttggtcg tcgtaccggt
     7021 gatctgggca aagaagatca cgaagattgt acctgtgata tgtgcattat ctttggcaat
     7081 gagcatgaga gcagcaaaat tcgttttgaa gatctggaac tgatcaacgg caacgaattt
     7141 gaaaagctgg aaaaacatat tgaccacgtg gccattgatc gttttacagg tggcgcactg
     7201 gacaaagcaa aatttgatac ctatccgctg gcaggtagcc cgaaaaaacc gctgaaactg
     7261 aagggtcgct tttggattaa aaagggtttt agcggtgatc acaagctgct gattaccaca
     7321 gcactgagcg atattcgtga tggcctgtat cctctgggta gtaaaggtgg tgttggttat
     7381 ggttgggttg caggtattag cattgatgat aatgtgccgg atgactttaa agagatgatc
     7441 aacaagacag aaatgccgct gccggaagaa gtggaagaaa gcaataatgg tccgatcaat
     7501 aacgattatg ttcatccggg tcatcagagc ccgaaacagg atcataaaaa caagaacatc
     7561 tattatccgc attattttct ggacagcggc agcaaagtgt atcgcgaaaa agatattatc
     7621 acccacgaag aattcaccga ggaactgctg tcaggcaaaa ttaactgtaa acttgaaacc
     7681 ctgacaccgc tgattattcc ggataccagt gatgaaaatg gtctgaaact tcagggtaat
     7741 aaaccgggtc ataagaacta caaattcttc aacattaatg gcgaactgat gattccgggt
     7801 tcagaactgc gtggcatgct gcgcacccat tttgaagcac tgaccaaaag ctgttttgcc
     7861 atttttggtg aagatagcac cctgagctgg cgtatgaatg cagatgagaa agattacaaa
     7921 atcgatagca acagcatccg caaaatggaa agccagcgta atccgaaata tcgcattccg
     7981 gacgaactgc agaaagagct gcgtaatagc ggtaatggtc tgtttaatcg tctgtatacc
     8041 agcgaacgtc gtttttggag tgatgtgagt aacaaatttg agaacagcat cgattacaaa
     8101 cgcgaaattc tgcgttgtgc aggtcgtccg aaaaactata aaggcggtat tattcgtcag
     8161 cgtaaagata gtctgatggc cgaagaactg aaagttcatc gtctgcctct gtatgataac
     8221 tttgatattc ctgatagcgc ctacaaagcc aacgatcatt gtcgtaaaag cgcaacctgt
     8281 agcaccagcc gtggttgtcg tgaacgtttt acctgtggca ttaaagtgcg tgataaaaat
     8341 cgcgtttttc tgaatgcagc caataataat cgccagtacc tgaacaacat caaaaagtcc
     8401 aatcacgatc tgtatctgca gtatctgaaa ggcgaaaaaa agatccgctt caacagcaaa
     8461 gttattacag gtagcgaacg tagcccgatt gatgttattg cagaactgaa tgaacgtggt
     8521 cgtcagaccg gttttatcaa actgagcggt ctgaataaca gcaataaaag ccagggcaat
     8581 accggcacca catttaatag tggttgggat cgctttgaac tgaatatact gctggatgat
     8641 ctggaaaccc gtccgagcaa aagcgattat ccgcgtccgc gtctgctgtt taccaaagat
     8701 cagtatgaat acaacatcac caaacgttgc gaacgcgtgt ttgaaattga taaaggcaac
     8761 aaaacaggct atccggtgga tgatcagatc aaaaagaact acgaagatat cctggacagc
     8821 tatgatggca tcaaagatca agaagttgcc gaacgctttg atacatttac ccgtggtagc
     8881 aagctgaaag ttggcgatct ggtttatttt catatcgacg gcgataacaa aattgacagc
     8941 ctgattccgg ttcgtattag ccgtaaatgt gcaagcaaaa ccttaggtgg caaattagat
     9001 aaagcactgc atccgtgcac cggtctgtca gatggtctgt gtccgggttg tcacctgttt
     9061 ggcaccaccg attataaagg tcgcgttaaa tttggcttcg ccaaatatga aaacggtcct
     9121 gaatggctga ttacgcgtgg taataatccg gaacgtagtc tgaccctggg tgtgctggaa
     9181 tcaccgcgtc cggcattttc aattccggat gatgaaagtg aaattccggg tcgtaaattc
     9241 tatctgcatc acaatggttg gcgcattatt cgccagaaac aactggaaat tcgtgaaacc
     9301 gttcagccgg aacgcaatgt taccaccgaa gtgatggata aaggtaacgt gtttagcttt
     9361 gatgtgcgct ttgaaaatct gcgtgaatgg gaactgggtc tgctgctgca gagtctggat
     9421 cctggtaaaa acattgcaca taaacttggt aaaggcaaac cgtatggttt tggcagcgtg
     9481 aaaatcaaga ttgatagcct gcataccttc aagattaaca gcaacaacga caaaatcaaa
     9541 cgtgttccgc agagtgatat ccgcgagtat attaacaaag gctaccagaa actgattgaa
     9601 tggtcaggta ataatagcat ccagaaaggt aatgtgctgc cgcagtggca tgttattccg
     9661 catattgaca aactgtacaa actgctgtgg gttccgtttc tgaacgatag caaactggaa
     9721 ccggatgttc gttatccggt tctgaatgaa gaatccaaag gttatattga gggcagcgat
     9781 tacacctata aaaagctggg agataaagat aacctgccgt ataaaacccg tgttaaaggt
     9841 ctgaccacac cgtggtcacc gtggaatccg tttcaggtga ttgccgaaca tgaagaacaa
     9901 gaagtgaacg ttaccggtag ccgtccgagt gttaccgata aaattgaacg tgatggtaaa
     9961 atggtgtaat aataac
//
