LOCUS       sequence-354268-         839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 
            (Omicron) variant with single chain Fc, HRV3C protease cleavage 
            site, and AVI tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Teng IT, Nazzari AF, Choe M, Liu T, Oliveira de Souza M, Petrova Y, 
            Tsybovsky Y, Wang S, Zhang B, Artamonov M, Madan B, Huang A, Lopez 
            Acevedo SN, Pan X, Ruckwardt TJ, DeKosky BJ, Mascola JR, Misasi J, 
            Sullivan NJ, Zhou T, Kwong PD
  TITLE     Molecular probes of spike ectodomain and its subdomains for 
            SARS-CoV-2 variants, Alpha through Omicron.
  JOURNAL   PLoS One. 2022 May 24;17(5):e0268767. doi: 
            10.1371/journal.pone.0268767. eCollection 2022.
  PUBMED    35609088
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2022-05-24"; volume: "17(5):e0268767. doi"; pages: " 
            10.1371/journal.pone.0268767. eCollection 2022"
COMMENT     Sequence Label: pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             129..152
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             774..818
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 ccagatggca gcagggcaat gtgttcagct gcagcgtgat gcacgaggct ctgcacaacc
       61 actacaccca gaaaagcctg tccctgagtc caggcaaggg cggctctggc ggaggaggat
      121 ctggcggcct ggaagtcctc ttccagggcc ctcccaatat cacaaacctg tgtcctttcg
      181 acgaggtgtt taacgccaca agattcgcca gcgtttatgc ctggaataga aaaagaatct
      241 ctaactgcgt ggctgactac agcgtgctgt ataacctggc cccttttttc accttcaagt
      301 gctacggcgt ttcccctaca aaactgaacg acctgtgctt cacgaacgtg tatgccgaca
      361 gcttcgtgat ccggggagat gaggtgcggc agatcgcccc tggccaaaca ggcaacatcg
      421 ccgattacaa ctacaagctg cccgacgact tcacaggatg tgtgatcgcc tggaacagca
      481 acaagctcga cagcaaagtg agcggaaact acaactacct gtacagactg tttagaaaga
      541 gcaatctgaa gcccttcgag agagatatca gcaccgagat ctaccaggcc ggcaacaaac
      601 cgtgcaacgg cgtggccggc ttcaactgct acttccccct gagaagctac tctttccggc
      661 ctacctacgg cgtgggccac cagccttaca gagtggtggt gctgagcttc gagctgctgc
      721 acgcccctgc caccgtgtgc ggaggcggcg gcctggtgcc ccagcagagc ggcggactga
      781 acgacatctt cgaggcccag aagatcgagt ggcacgaggg ctgatgagga tccagatct
//
