LOCUS       sequence-354286-       11344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PASTEv1 PE2-P2A-Bxb1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY0216 PASTEv1 PE2-P2A-Bxb1
FEATURES             Location/Qualifiers
     source          1..11344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     795..815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     886..905
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        886..904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             954..974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(975..3488,3489..3491,3492..5075)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             975..5075
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7248..7268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7278..7334
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7335..7355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7356..7382
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8908..8925)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8914..9138
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9209..9225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9209..9225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9222..9244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9233..9249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9257..9263,9264..9281,9282..9287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9302..9323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9440..9457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9611..10199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9691..9710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10370..11161,11162..11230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10993..11012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11231..11335)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
       61 tactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      901 tagggagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga
      961 agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
     1021 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     1081 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     1141 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     1201 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     1261 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     1321 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     1381 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     1441 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     1501 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     1561 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1621 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     1681 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     1741 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     1801 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     1861 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     1921 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     1981 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     2041 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     2101 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     2161 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     2221 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     2281 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     2341 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     2401 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     2461 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     2521 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     2581 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     2641 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     2701 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     2761 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     2821 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     2881 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     2941 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     3001 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     3061 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     3121 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     3181 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     3241 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     3301 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     3361 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     3421 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     3481 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     3541 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3601 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     3661 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     3721 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     3781 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     3841 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     3901 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     3961 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     4021 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     4081 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     4141 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     4201 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     4261 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     4321 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     4381 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     4441 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     4501 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     4561 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4621 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     4681 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     4741 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     4801 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     4861 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     4921 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     4981 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     5041 agacacggat cgacctgtct cagctgggag gtgactctgg aggatctagc ggaggatcct
     5101 ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca
     5161 gcggcggcag cagcacccta aatatagaag atgagtatcg gctacatgag acctcaaaag
     5221 agccagatgt ttctctaggg tccacatggc tgtctgattt tcctcaggcc tgggcggaaa
     5281 ccgggggcat gggactggca gttcgccaag ctcctctgat catacctctg aaagcaacct
     5341 ctacccccgt gtccataaaa caatacccca tgtcacaaga agccagactg gggatcaagc
     5401 cccacataca gagactgttg gaccagggaa tactggtacc ctgccagtcc ccctggaaca
     5461 cgcccctgct acccgttaag aaaccaggga ctaatgatta taggcctgtc caggatctga
     5521 gagaagtcaa caagcgggtg gaagacatcc accccaccgt gcccaaccct tacaacctct
     5581 tgagcgggct cccaccgtcc caccagtggt acactgtgct tgatttaaag gatgcctttt
     5641 tctgcctgag actccacccc accagtcagc ctctcttcgc ctttgagtgg agagatccag
     5701 agatgggaat ctcaggacaa ttgacctgga ccagactccc acagggtttc aaaaacagtc
     5761 ccaccctgtt taatgaggca ctgcacagag acctagcaga cttccggatc cagcacccag
     5821 acttgatcct gctacagtac gtggatgact tactgctggc cgccacttct gagctagact
     5881 gccaacaagg tactcgggcc ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg
     5941 ccaagaaagc ccaaatttgc cagaaacagg tcaagtatct ggggtatctt ctaaaagagg
     6001 gtcagagatg gctgactgag gccagaaaag agactgtgat ggggcagcct actccgaaga
     6061 cccctcgaca actaagggag ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg
     6121 ggtttgcaga aatggcagcc cccctgtacc ctctcaccaa accggggact ctgtttaatt
     6181 ggggcccaga ccaacaaaag gcctatcaag aaatcaagca agctcttcta actgccccag
     6241 ccctggggtt gccagatttg actaagccct ttgaactctt tgtcgacgag aagcagggct
     6301 acgccaaagg tgtcctaacg caaaaactgg gaccttggcg tcggccggtg gcctacctgt
     6361 ccaaaaagct agacccagta gcagctgggt ggcccccttg cctacggatg gtagcagcca
     6421 ttgccgtact gacaaaggat gcaggcaagc taaccatggg acagccacta gtcattctgg
     6481 ccccccatgc agtagaggca ctagtcaaac aaccccccga ccgctggctt tccaacgccc
     6541 ggatgactca ctatcaggcc ttgcttttgg acacggaccg ggtccagttc ggaccggtgg
     6601 tagccctgaa cccggctacg ctgctcccac tgcctgagga agggctgcaa cacaactgcc
     6661 ttgatatcct ggccgaagcc cacggaaccc gacccgacct aacggaccag ccgctcccag
     6721 acgccgacca cacctggtac acggatggaa gcagtctctt acaagaggga cagcgtaagg
     6781 cgggagctgc ggtgaccacc gagaccgagg taatctgggc taaagccctg ccagccggga
     6841 catccgctca gcgggctgaa ctgatagcac tcacccaggc cctaaagatg gcagaaggta
     6901 agaagctaaa tgtttatact gatagccgtt atgcttttgc tactgcccat atccatggag
     6961 aaatatacag aaggcgtggg tggctcacat cagaaggcaa agagatcaaa aataaagacg
     7021 agatcttggc cctactaaaa gccctctttc tgcccaaaag acttagcata atccattgtc
     7081 caggacatca aaagggacac agcgccgagg ctagaggcaa ccggatggct gaccaagcgg
     7141 cccgaaaggc agccatcaca gagactccag acacctctac cctcctcata gaaaattcat
     7201 caccctctgg cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga
     7261 ggaaagtcgg aagcggagct actaacttca gcctgctgaa gcaggctggc gacgtggagg
     7321 agaaccctgg acctccaaaa aagaaaagaa aagtgtatcc ctatgatgtc cccgattatg
     7381 ccggttcaag agccctggtc gtgattagac tgagccgagt gacagacgcc accacaagtc
     7441 ccgagagaca gctggaatca tgccagcagc tctgtgctca gcggggttgg gatgtggtcg
     7501 gcgtggcaga ggatctggac gtgagcgggg ccgtcgatcc attcgacaga aagaggaggc
     7561 ccaacctggc aagatggctc gctttcgagg aacagccctt tgatgtgatc gtcgcctaca
     7621 gagtggaccg gctgacccgc tcaattcgac atctccagca gctggtgcat tgggctgagg
     7681 accacaagaa actggtggtc agcgcaacag aagcccactt cgatactacc acaccttttg
     7741 ccgctgtggt catcgcactg atgggcactg tggcccagat ggagctcgaa gctatcaagg
     7801 agcgaaacag gagcgcagcc catttcaata ttagggccgg taaatacaga ggctccctgc
     7861 ccccttgggg atatctccct accagggtgg atggggagtg gagactggtg ccagaccccg
     7921 tccagagaga gcggattctg gaagtgtacc acagagtggt cgataaccac gaaccactcc
     7981 atctggtggc acacgacctg aatagacgcg gcgtgctctc tccaaaggat tattttgctc
     8041 agctgcaggg aagagagcca cagggaagag aatggagtgc tactgcactg aagagatcta
     8101 tgatcagtga ggctatgctg ggttacgcaa cactcaatgg caaaactgtc cgggacgatg
     8161 acggagcccc tctggtgagg gctgagccta ttctcaccag agagcagctc gaagctctgc
     8221 gggcagaact ggtcaagact agtcgcgcca aacctgccgt gagcacccca agcctgctcc
     8281 tgagggtgct gttctgcgcc gtctgtggag agccagcata caagtttgcc ggcggagggc
     8341 gcaaacatcc ccgctatcga tgcaggagca tggggttccc taagcactgt ggaaacggga
     8401 cagtggccat ggctgagtgg gacgcctttt gcgaggaaca ggtgctggat ctcctgggtg
     8461 acgctgagcg gctggaaaaa gtgtgggtgg caggatctga ctccgctgtg gagctggcag
     8521 aagtcaatgc cgagctcgtg gatctgactt ccctcatcgg atctcctgca tatagagctg
     8581 ggtccccaca gagagaagct ctggacgcac gaattgctgc actcgctgct agacaggagg
     8641 aactggaggg cctggaggcc aggccctctg gatgggagtg gcgagaaacc ggacagaggt
     8701 ttggggattg gtggagggag caggacaccg cagccaagaa cacatggctg agatccatga
     8761 atgtccggct cacattcgac gtgcgcggtg gcctgactcg aaccatcgat tttggcgacc
     8821 tgcaggagta tgaacagcac ctgagactgg ggtccgtggt cgaaagactg cacactggga
     8881 tgtcctaggt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     8941 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9001 cctaataaaa tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9061 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     9121 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
     9181 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9241 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
     9301 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9361 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10201 aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag
    10261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11341 ttcc
//
