LOCUS       sequence-354293-        2550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCP-SoxS (R93A) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Tickman BI, Burbano DA, Chavali VP, Kiattisewee C, Fontana J, 
            Khakimzhan A, Noireaux V, Zalatan JG, Carothers JM
  TITLE     Multi-layer CRISPRa/i circuits for dynamic genetic programs in 
            cell-free and bacterial systems.
  JOURNAL   Cell Syst. 2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 
            10.1016/j.cels.2021.10.008.
  PUBMED    34800362
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 
            10.1016/j.cels.2021.10.008."
COMMENT     Sequence Label: pBT005
FEATURES             Location/Qualifiers
     source          1..2550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..41
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             51..62
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(830..847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(964..1509)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1044..1063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1602..1696)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1717..2376)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2291..2310
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2377..2479)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctgcagttta cggctagctc agccctaggt attatgctag cgaattcatt aaagaggaga
       61 aaggtaccat ggggcccgct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa
      121 ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg gatcgctgaa tggatcagct
      181 ctaactcgcg ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga
      241 atcgcaaata caccatcaaa gtcgaggtgc ctaaaggcgc ctggcgttcg tacttaaata
      301 tggaactaac cattccaatt ttcgccacga attccgactg cgagcttatt gttaaggcaa
      361 tgcaaggtct cctaaaagat ggaaacccga ttccctcagc aatcgcagca aactccggca
      421 tctacggtgg cggaggtagc atgtcccatc agaaaattat tcaggatctt atcgcatgga
      481 ttgacgagca tattgaccag ccgcttaaca ttgatgtagt cgcaaaaaaa tcaggctatt
      541 caaagtggta cttgcaacga atgttccgca cggtgacgca tcagacgctt ggcgattaca
      601 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
      661 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttcgcgc
      721 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtaagcggcc gccacgcaaa
      781 aaaccccgct tcggcggggt tttttcgccc tagggatata ttccgcttcc tcgctcactg
      841 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
      901 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
      961 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     1021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     1081 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     1141 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     1201 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1261 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     1321 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     1381 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     1441 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     1501 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     1561 atctcttcaa atgtagcacc tgaagtcagc ctagtgcttg gattctcacc aataaaaaac
     1621 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1681 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1741 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1801 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1861 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1921 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     1981 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2041 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2101 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2161 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2221 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2281 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2341 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2401 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2461 acctcttacg tgccgatcaa cgtctcattt tcgcctaatt cctaattttt gttggagcgc
     2521 ccggaagaga gtcaattcag ggtggtgaat
//
