LOCUS       sequence-354312-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for CRISPRa-SAM with GARP targeting 
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, 
            Blaeschke F, Ye CJ, Marson A
  TITLE     CRISPR activation and interference screens decode stimulation 
            responses in primary human T cells.
  JOURNAL   Science. 2022 Feb 4;375(6580):eabj4008. doi: 
            10.1126/science.abj4008. Epub 2022 Feb 4.
  PUBMED    35113687
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-02-4"; volume: "375(6580):eabj4008. doi"; pages: " 
            10.1126/science.abj4008. Epub 2022 Feb 4"
COMMENT     Sequence Label: 
            pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..203
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    250..375
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    868..1101
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1286..1330
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1508..1756
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1508..1528
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1679..1698
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2025..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2191..2701
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2620..2638
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3166..3190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3240..3260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3276..3818
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             3843..4214
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             4230..4283
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4311..4907
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4311..4330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4807..4827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4997..5230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5308..5429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5345..5364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5399..5418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5469..5604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5624..5643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5625..5643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5653..5670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5653..5669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5662..5684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5811..6266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5898..5917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6108..6129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6292..6396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6397..6465,6466..7257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6615..6634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7428..8016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7917..7936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8170..8187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8304..8325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8340..8345,8346..8363,8364..8370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8378..8394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8383..8405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8402..8418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8402..8418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8437..8457
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8439..8457
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(8485..8689,1..22)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
       61 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      181 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      241 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      301 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      361 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      421 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      481 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      541 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      601 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      661 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
      721 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
      781 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
      841 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
      901 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
      961 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1021 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1081 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1141 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1201 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1261 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1321 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1381 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1441 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1501 acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     1561 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     1621 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     1681 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     1741 gaaaggacga aacaccgaaa ttgcagccgg agcgcgggtt taagagctat gctgcgaata
     1801 cgagggagca gacgatatgg cgtcgctcct ctccacgaga gcatatgggc tccgtggtct
     1861 cgtacaccat cagggtacgt atcagacacc atcagggtct gctcgtattc gcagcatagc
     1921 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     1981 tttgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2041 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2101 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2161 agcagagatc cactttggcg ccggctcgag ggggcccggg ttgcgccttt tccaaggcag
     2221 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     2281 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     2341 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     2401 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtcttacta gtaccctcgc
     2461 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     2521 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     2581 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     2641 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     2701 gggggatcca acttaagctt gccaccatgt cgcggaggcg gcacagctac gagaacgatg
     2761 gtggacaacc tcacaaaagg aggaagacgt ctatgctagc ctccaaaacc atcgttcttt
     2821 cggtcggcga ggctactcgc actctgactg agatccagtc caccgcagac cgtcagatct
     2881 tcgaagagaa ggtcgggcct ctggtgggtc ggctgcgcct cacggcttcg ctccgtcaaa
     2941 acggagccaa gaccgcgtat cgagtcaacc taaaactgga tcaggcggac gtcgttgatt
     3001 ccggacttcc gaaagtgcgc tacactcagg tatggtcgca cgacgtgaca atcgttgcga
     3061 atagcaccga ggcctcgcgc aaatcgttgt acgatttgac caagtccctc gtcgcgacct
     3121 cgcaggtcga agatcttgtc gtcaaccttg tgccgctggg ccgttcttca actttcttgt
     3181 acaaagtggg cggaggaggt ggaagcggag gaggaggaag cggaggagga ggtagcggac
     3241 ctaagaaaaa gaggaaggtg gcggccgctg gtaccccttc agggcagatc agcaaccagg
     3301 ccctggctct ggcccctagc tccgctccag tgctggccca gactatggtg ccctctagtg
     3361 ctatggtgcc tctggcccag ccacctgctc cagcccctgt gctgacccca ggaccacccc
     3421 agtcactgag cgctccagtg cccaagtcta cacaggccgg cgaggggact ctgagtgaag
     3481 ctctgctgca cctgcagttc gacgctgatg aggacctggg agctctgctg gggaacagca
     3541 ccgatcccgg agtgttcaca gatctggcct ccgtggacaa ctctgagttt cagcagctgc
     3601 tgaatcaggg cgtgtccatg tctcatagta cagccgaacc aatgctgatg gagtaccccg
     3661 aagccattac ccggctggtg accggcagcc agcggccccc cgaccccgct ccaactcccc
     3721 tgggaaccag cggcctgcct aatgggctgt ccggagatga agacttctca agcatcgctg
     3781 atatggactt tagtgccctg ctgtcacaga tttcctctag tgggcaggga ggaggtggaa
     3841 gcggcttcag cgtggacacc agtgccctgc tggacctgtt cagcccctcg gtgaccgtgc
     3901 ccgacatgag cctgcctgac cttgacagca gcctggccag tatccaagag ctcctgtctc
     3961 cccaggagcc ccccaggcct cccgaggcag agaacagcag cccggattca gggaagcagc
     4021 tggtgcacta cacagcgcag ccgctgttcc tgctggaccc cggctccgtg gacaccggga
     4081 gcaacgacct gccggtgctg tttgagctgg gagagggctc ctacttctcc gaaggggacg
     4141 gcttcgccga ggaccccacc atctccctgc tgacaggctc ggagcctccc aaagccaagg
     4201 accccactgt ctccgatatc ggcagtggag agggcagagg aagtctgcta acatgcggtg
     4261 acgtcgagga gaatcctggc ccaggtacca cagatccacc ggagcttacc atgaccgagt
     4321 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4381 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4441 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4501 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
     4561 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4621 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4681 tcctggccac cgtcggcgtt tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4741 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4801 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4861 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgct agctgaacgc
     4921 gttaagtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5041 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5221 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     5281 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5401 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5521 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5581 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     5641 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5701 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5761 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     5821 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5881 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5941 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6001 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6061 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6121 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6181 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6241 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6601 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8101 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8161 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8221 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8281 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8341 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8401 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8461 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8521 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8581 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8641 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaa
//
