LOCUS       sequence-354317-        8707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture 
            sequence, and PCP-P65-HSF1 cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, 
            Blaeschke F, Ye CJ, Marson A
  TITLE     CRISPR activation and interference screens decode stimulation 
            responses in primary human T cells.
  JOURNAL   Science. 2022 Feb 4;375(6580):eabj4008. doi: 
            10.1126/science.abj4008. Epub 2022 Feb 4.
  PUBMED    35113687
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-02-4"; volume: "375(6580):eabj4008. doi"; pages: " 
            10.1126/science.abj4008. Epub 2022 Feb 4"
COMMENT     Sequence Label: 
            pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro
FEATURES             Location/Qualifiers
     source          1..8707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..203
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    250..375
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    868..1101
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1286..1330
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1508..1756
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1508..1528
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1679..1698
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2043..2160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2209..2719
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2638..2656
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3184..3208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3258..3278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3294..3836
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             3861..4232
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             4248..4301
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4329..4925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4329..4348)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4825..4845
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5015..5248
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5326..5447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5363..5382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5417..5436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5487..5622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5642..5661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5643..5661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5671..5688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5671..5687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5680..5702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5829..6284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5916..5935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6126..6147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6310..6414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6415..6483,6484..7275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6633..6652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7446..8034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7935..7954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8188..8205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8322..8343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8358..8363,8364..8381,8382..8388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8396..8412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8401..8423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8420..8436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8420..8436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8455..8475
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8457..8475
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(8503..8707,1..22)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
       61 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      181 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      241 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      301 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      361 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      421 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      481 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      541 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      601 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      661 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
      721 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
      781 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
      841 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
      901 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
      961 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1021 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1081 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1141 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1201 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1261 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1321 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1381 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1441 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1501 acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     1561 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     1621 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     1681 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     1741 gaaaggacga aacaccggag acgaccggtt ttttgcgtct ctgtttaaga gctatgctgc
     1801 gaatacgagg gagcagacga tatggcgtcg ctcctctcca cgagagcata tgggctccgt
     1861 ggtctcgtac gctttaaggc cggtcctagc aagtacgtat cagacaccat cagggtctgc
     1921 tcgtattcgc agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     1981 caccgagtcg gtgctttttt tgaattctag atcttgagac aaatggcagt attcatccac
     2041 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2101 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2161 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtt
     2221 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2281 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2341 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2401 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2461 tcttactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2521 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2581 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2641 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2701 caccgacctc tctccccagg gggatccaac ttaagcttgc caccatgtcg cggaggcggc
     2761 acagctacga gaacgatggt ggacaacctc acaaaaggag gaagacgtct atgctagcct
     2821 ccaaaaccat cgttctttcg gtcggcgagg ctactcgcac tctgactgag atccagtcca
     2881 ccgcagaccg tcagatcttc gaagagaagg tcgggcctct ggtgggtcgg ctgcgcctca
     2941 cggcttcgct ccgtcaaaac ggagccaaga ccgcgtatcg agtcaaccta aaactggatc
     3001 aggcggacgt cgttgattcc ggacttccga aagtgcgcta cactcaggta tggtcgcacg
     3061 acgtgacaat cgttgcgaat agcaccgagg cctcgcgcaa atcgttgtac gatttgacca
     3121 agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc
     3181 gttcttcaac tttcttgtac aaagtgggcg gaggaggtgg aagcggagga ggaggaagcg
     3241 gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctggt accccttcag
     3301 ggcagatcag caaccaggcc ctggctctgg cccctagctc cgctccagtg ctggcccaga
     3361 ctatggtgcc ctctagtgct atggtgcctc tggcccagcc acctgctcca gcccctgtgc
     3421 tgaccccagg accaccccag tcactgagcg ctccagtgcc caagtctaca caggccggcg
     3481 aggggactct gagtgaagct ctgctgcacc tgcagttcga cgctgatgag gacctgggag
     3541 ctctgctggg gaacagcacc gatcccggag tgttcacaga tctggcctcc gtggacaact
     3601 ctgagtttca gcagctgctg aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa
     3661 tgctgatgga gtaccccgaa gccattaccc ggctggtgac cggcagccag cggccccccg
     3721 accccgctcc aactcccctg ggaaccagcg gcctgcctaa tgggctgtcc ggagatgaag
     3781 acttctcaag catcgctgat atggacttta gtgccctgct gtcacagatt tcctctagtg
     3841 ggcagggagg aggtggaagc ggcttcagcg tggacaccag tgccctgctg gacctgttca
     3901 gcccctcggt gaccgtgccc gacatgagcc tgcctgacct tgacagcagc ctggccagta
     3961 tccaagagct cctgtctccc caggagcccc ccaggcctcc cgaggcagag aacagcagcc
     4021 cggattcagg gaagcagctg gtgcactaca cagcgcagcc gctgttcctg ctggaccccg
     4081 gctccgtgga caccgggagc aacgacctgc cggtgctgtt tgagctggga gagggctcct
     4141 acttctccga aggggacggc ttcgccgagg accccaccat ctccctgctg acaggctcgg
     4201 agcctcccaa agccaaggac cccactgtct ccgatatcgg cagtggagag ggcagaggaa
     4261 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggtaccaca gatccaccgg
     4321 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     4381 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4441 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4501 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
     4561 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4621 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4681 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtttc gcccgaccac cagggcaagg
     4741 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4801 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     4861 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     4921 gtgccgctag ctgaacgcgt taagtcgact ttaagaccaa tgacttacaa ggcagctgta
     4981 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5041 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5101 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5161 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5221 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     5281 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     5341 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5401 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     5461 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5521 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     5641 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     5701 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5761 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5821 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5881 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5941 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6001 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6061 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6121 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6181 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6241 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6301 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6361 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6421 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6481 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6541 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6601 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6661 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6721 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6781 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6841 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6901 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6961 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7021 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7081 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7141 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7201 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7261 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7321 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7561 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8101 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8221 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8281 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8341 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8401 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     8461 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     8521 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     8581 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     8641 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     8701 tatttaa
//
