LOCUS       sequence-354327-       20404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) 
            but with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20404)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 20404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelNS1-mCherry
FEATURES             Location/Qualifiers
     source          1..20404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..43
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    61..459
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    719..1192
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1265..2236)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2236..3411)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3737..3987
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3990..4745)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4836..5055
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5123..5654
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5131..5745
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6603..6705
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6706..7365
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6772..6791)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7585..7606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7621..7626,7627..7644,7645..7651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7664..7686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7683..7699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7683..7699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8136..8154
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8136..8153
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             8353..9060
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8498..8516)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8766..8785
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8960..8983
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     19694..19713
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(20077..20096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(20078..20096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(20103..20120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(20103..20119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20112..20134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(20327..20346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatcgaata taacttcgta taatgtatgc tatacgaagt tattagcgat gagctcggac
       61 ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagctc
      121 gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
      181 tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
      241 gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
      301 ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
      361 tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaagcaa cttcagacaa
      421 tacaaatcag cgacactgaa tacggggcaa cctcatgtcc gagctcgcga gctcgtcgac
      481 agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag
      541 gtattttgtc cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc
      601 cacagcaggc atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt
      661 caatacttgc ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga
      721 caatacaagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg
      781 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
      841 tcagaccgac gatacgagtg ggaccgtggt tccagactaa taatcagacc gacgatacga
      901 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac catggtccca
      961 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     1021 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     1081 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     1141 atcagaccga cgatacaagt ggaacagtgg gcccagagag aatattcagg ccagttatgc
     1201 tttctggcct gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt
     1261 cgcatcaggg tgctggcttt tcaagttcct taagaatggc ctcaattttc tctatacact
     1321 cagttggaac acgagacctg tccaggttaa gcaccatttt atcgccctta tacaatactg
     1381 tcgctccagg agcaaactga tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt
     1441 taagcacaga agttaaaaga gtgataactt cttcagcttc aaatatcacc ccagcttttt
     1501 tctgctcatg aaggttagat gcctgctgct taagtaattc ctctttatct gtaaaggctt
     1561 tttgaagtgc atcacctgac cgggcagata gttcaccggg gtgagaaaaa agagcaacaa
     1621 ctgatttagg caatttggcg gtgttgatac agcgggtaat aatcttacgt gaaatatttt
     1681 ccgcatcagc cagcgcagaa atatttccag caaattcatt ctgcaatcgg cttgcataac
     1741 gctgaccacg ttcataagca cttgttgggc gataatcgtt acccaatctg gataatgcag
     1801 ccatctgctc atcatccagc tcgccaacca gaacacgata atcactttcg gtaagtgcag
     1861 cagctttacg acggcgactc ccatcggcaa tttctatgac accagatact cttcgaccga
     1921 acgccggtgt ctgttgacca gtcagtagaa aagaagggat gagatcatcc agtgcgtcct
     1981 cagtaagcag ctcctggtca cgttcattac ctgaccatac ccgagaggtc ttctcaacac
     2041 tatcaccccg gagcacttca agagtaaact tcacatcccg accacataca ggcaaagtaa
     2101 tggcattacc gcgagccatt actcctacgc gcgcaattaa cgaatccacc atcggggcag
     2161 ctggtgtcga taacgaagta tcttcaaccg gttgagtatt gagcgtatgt tttggaataa
     2221 caggcgcacg cttcattatc taatctccca gcgtggttta atcagacgat cgaaaatttc
     2281 attgcagaca ggttcccaaa tagaaagagc atttctccag gcaccagttg aagagcgttg
     2341 atcaatggcc tgttcaaaaa cagttctcat ccggatctga cctttaccaa cttcatccgt
     2401 ttcacgtaca acatttttta gaaccatgct tccccaggca tcccgaattt gctcctccat
     2461 ccacggggac tgagagccat tactattgct gtatttggta agcaaaatac gtacatcagg
     2521 ctcgaaccct ttaagatcaa cgttcttgag cagatcacga agcatatcga aaaactgcag
     2581 tgcggaggtg tagtcaaaca actcagcagg cgtgggaaca atcagcacat cagcagcaca
     2641 tacgacatta atcgtgccga tacccaggtt aggcgcgctg tcaataacta tgacatcata
     2701 gtcatgagca acagtttcaa tggccagtcg gagcatcagg tgtggatcgg tgggcagttt
     2761 accttcatca aatttgccca ttaactcagt ttcaatacgg tgcagagcca gacaggaagg
     2821 aataatgtca agccccggcc agcaagtggg ctttattgca taagtgacat cgtccttttc
     2881 cccaagatag aaaggcagga gagtgtcttc tgcatgaata tgaagatctg gtacccatcc
     2941 gtgatacatt gaggctgttc cctgggggtc gttaccttcc acgagcaaaa cacgtagccc
     3001 cttcagagcc agatcctgag caagatgaac agaaactgag gttttgtaaa cgccaccttt
     3061 atgggcagca accccgatca ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc
     3121 aaacacatca cgcatatgat taatttgttc aattgtataa ccaacacgtt gctcaacccg
     3181 tcctcgaatt tccatatccg ggtgcggtag tcgccctgct ttctcggcat ctctgatagc
     3241 ctgagaagaa accccaacta aatccgctgc ttcacctatt ctccagcgcc gggttatttt
     3301 cctcgcttcc gggctgtcat cattaaactg tgcaatggcg atagccttcg tcatttcatg
     3361 accagcgttt atgcactggt taagtgtttc catgagtttc attctgaaca tcctttaatc
     3421 attgctttgc gtttttttat taaatcttgc aatttactgc aaagcaacaa caaaatcgca
     3481 aagtcatcaa aaaaccgcaa agttgtttaa aataagagca acactacaaa aggagataag
     3541 aagagcacat acctcagtca cttattatca ctagcgctcg ccgcagccgt gtaaccgagc
     3601 atagcgagcg aactggcgag gaagcaaaga agaactgttc tgtcagatag ctcttacgct
     3661 cagcgcaaga agaaatatcc accgtgggaa aaactccagg tagaggtaca cacgcggata
     3721 gccaattcag agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc
     3781 cgatatcaag tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc
     3841 aaatttggct tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa
     3901 ggaaacagca aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac
     3961 cctcaaatct gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg
     4021 aaggaaaata cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc
     4081 attggagttc tgctgttgat ctcattaaca cagacctgca ggaagcggcg gcggaagtca
     4141 ggcatacgct ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga
     4201 gagacgatgc ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg
     4261 gcatacggat tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt
     4321 aacccgataa agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat
     4381 ggactgtgcg cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca
     4441 tcctcttcag ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt
     4501 atatccttac tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg
     4561 atctcgcaga taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg
     4621 aacagataca gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg
     4681 tttgactgga cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc
     4741 gccatgacag atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag
     4801 agtcaataag cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca
     4861 gggttaaggg caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa
     4921 agggcaattt gtcacaacac cttctctaga accagcatgg ataaaggcct acaaggcgct
     4981 ctaaaaaaga agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggataa
     5041 gtggataacc ccaagggaag ttttttcagg catcgtgtgt aagcagaata tataagtgct
     5101 gttccctggt gcttcctcgc tcactcgacc gggagggttc gagaaggggg ggcacccccc
     5161 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     5221 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     5281 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     5341 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     5401 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     5461 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     5521 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     5581 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     5641 caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     5701 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagccgag ggcttcgccc
     5761 tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat catggttctg
     5821 tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa cgtaacaccg
     5881 cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg
     5941 catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat taataatgcg
     6001 gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag tggataacag
     6061 ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca tttccatata
     6121 aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca tcagactccg
     6181 gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga
     6241 cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc agcggtatac
     6301 cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg accggaacag
     6361 agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag gtcctgaccg
     6421 ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga cggttacgcc
     6481 gctccatgag cttatcgcga ataaatacct gtgacggaag atcacttcgc agaataaata
     6541 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     6601 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     6661 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     6721 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     6781 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     6841 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     6901 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     6961 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7021 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7081 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7141 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7201 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7261 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     7321 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     7381 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     7441 ggcagaaatt cgatgataag ctgtcaaaca tgagaattgg tcgacggccc gggcggccgc
     7501 aaggagttcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7561 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7621 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7681 cacaggaaac agctatgacc atgattacgc caagctattt aggtgagact atagaatact
     7741 caagcttgca tgcctgcagg tcgactctag aggatcccac attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtgt cgacgcggcc gctagcgatg
     7921 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     7981 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
     8041 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
     8101 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagagtaaat
     8161 cctgtgtgct aattgaggtg cattggtctg caaatcgagt tgctaggcaa taaacacatt
     8221 tggattaatt ttaatcgttc gttgagcgat tagcagagaa ctgaccagaa catgtctggt
     8281 cgtaaagctc agggaaaaac cctgggcgtc aatatggtac gacgaggagt tcgctccttg
     8341 tcaaacgagc tcatggtgag caagggcgag gaggacaaca tggccatcat caaggagttc
     8401 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     8461 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     8521 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     8581 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     8641 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     8701 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     8761 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     8821 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     8881 cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     8941 gcctacaacg tcaacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     9001 gaacagtacg agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     9061 accggtaact ttgaccttct caagttggcc ggcgacgtcg agtccaaccc agggcccctg
     9121 cagcaaattt tcgtgaagac cctgacgggc aagaccatca ctcttgaggt cgagcccagt
     9181 gacaccatcg agaatgtcaa ggccaagatc caagacaagg aaggcatccc acctgaccag
     9241 cagaggctga tattcgcggg caaacagctg gaggatggcc gcaccctgtc cgactacaac
     9301 atccagaaag agtccacctt gcacctggtg ctgtgtctcc gcggtggaat gtccggtcgt
     9361 aaagcccagg gcaaaaccct aggcgtaaat atggtccgac gcggagtccg ctcattgtcg
     9421 aacaaaataa aacaaaaaac aaaacaaatt ggaaacagac ctggaccttc aagaggtgtt
     9481 caaggattta tctttttctt tttgttcaac attttgactg gaaaaaagat cacagcccac
     9541 ctaaagaggt tgtggaaaat gctggaccca agacaaggct tggctgttct aaggaaagtc
     9601 aagagagtgg tggccagttt gatgagagga ttgtcctcaa ggaaacgccg ttcccatgat
     9661 gttctgactg tgcaattcct aattttggga atgctgttga tgacgggtgg agtgaccttg
     9721 gtgcggaaaa acagatggtt gctcctaaat gtgacatctg aggacctcgg gaaaacattc
     9781 tctgtgggca caggcaactg cacaacaaac attttggaag ccaagtactg gtgcccagac
     9841 tcaatggaat acaactgtcc caatctcagt ccaagagagg agccagatga cattgattgc
     9901 tggtgctatg gggtggaaaa cgttagagtc gcatatggta agtgtgactc agcaggcagg
     9961 tctaggaggt caagaagggc cattgacttg cctacgcatg aaaaccatgg tttgaagacc
    10021 cggcaagaaa aatggatgac tggaagaatg ggtgaaaggc aactccaaaa gattgagaga
    10081 tggttcgtga ggaacccctt ttttgcagtg acggctctga ccattgccta ccttgtggga
    10141 agcaacatga cgcaacgagt cgtgattgcc ctactggtct tggctgttgg tccggcctac
    10201 tcagctcact gcattggaat tactgacagg gatttcattg agggggtgca tggaggaact
    10261 tgggtttcag ctaccctgga gcaagacaag tgtgtcactg ttatggcccc tgacaagcct
    10321 tcattggaca tctcactaga gacagtagcc attgatagac ctgctgaggt gaggaaagtg
    10381 tgttacaatg cagttctcac tcatgtgaag attaatgaca agtgccccag cactggagag
    10441 gcccacctag ccgaagagaa cgaaggggac aatgcgtgca agcgcactta ttctgataga
    10501 ggctggggca atggctgtgg cctatttggg aaagggagca ttgtggcatg cgccaaattc
    10561 acttgtgcca aatccatgag tttgtttgag gttgatcaga ccaaaattca gtatgtcatc
    10621 agagcacaat tgcatgtagg ggccaagcag gaaaattgga ataccgacat taagactctc
    10681 aagtttgatg ccctgtcagg ctcccaggaa gtcgagttca ttgggtatgg aaaagctaca
    10741 ctggaatgcc aggtgcaaac tgcggtggac tttggtaaca gttacatcgc tgagatggaa
    10801 acagagagct ggatagtgga cagacagtgg gcccaggact tgaccctgcc atggcagagt
    10861 ggaagtggcg gggtgtggag agagatgcat catcttgtcg aatttgaacc tccgcatgcc
    10921 gccactatca gagtactggc cctgggaaac caggaaggct ccttgaaaac agctcttact
    10981 ggcgcaatga gggttacaaa ggacacaaat gacaacaacc tttacaaact acatggtgga
    11041 catgtttctt gcagagtgaa attgtcagct ttgacactca aggggacatc ctacaaaata
    11101 tgcactgaca aaatgttttt tgtcaagaac ccaactgaca ctggccatgg cactgttgtg
    11161 atgcaggtga aagtgtcaaa aggagccccc tgcaggattc cagtgatagt agctgatgat
    11221 cttacagcgg caatcaataa aggcattttg gttacagtta accccatcgc ctcaaccaat
    11281 gatgatgaag tgctgattga ggtgaaccca ccttttggag acagctacat tatcgttggg
    11341 agaggagatt cacgtctcac ttaccagtgg cacaaagagg gaagctcaat aggaaagttg
    11401 ttcactcaga ccatgaaagg cgtggaacgc ctggccgtca tgggagacac cgcctgggat
    11461 ttcagctccg ctggagggtt cttcacttcg gttgggaaag gaattcatac ggtgtttggc
    11521 tctgcctttc aggggctatt tggcggcttg aactggataa caaaggtcat catgggggcg
    11581 gtacttatat gggttggcat caacacaaga aacatgacaa tgtccatgag catgatcttg
    11641 gtaggagtga tcatgatgtt tttgtctcta ggagttgggg cggatcaagg atgcgccatc
    11701 aactttggca agagagagct cggatcacgg tccgggacac gcgtgtacat ggacgcagtc
    11761 tttgaataca ccatagactg cgatggatct atcttgggtg cagcggtgaa cggaaaaaag
    11821 agtgcccatg gctctccaac attttggatg ggaagtcatg aagtaaatgg gacatggatg
    11881 atccacacct tggaggcatt agattacaag gagtgtgagt ggccactgac acatacgatt
    11941 ggaacatcag ttgaagagag tgaaatgttc atgccgagat caatcggagg cccagttagc
    12001 tctcacaatc atatccctgg atacaaggtt cagacgaacg gaccttggat gcaggtacca
    12061 ctagaagtga agagagaagc ttgcccaggg actagcgtga tcattgatgg caactgtgat
    12121 ggacggggaa aatcaaccag atccaccacg gatagcggga aagttattcc tgaatggtgt
    12181 tgccgctcct gcacaatgcc gcctgtgagc ttccatggta gtgatgggtg ttggtatccc
    12241 atggaaatta ggccaaggaa aacgcatgaa agccatctgg tgcgctcctg ggttacagct
    12301 ggagaaatac atgctgtccc ttttggtttg gtgagcatga tgatagcaat ggaagtggtc
    12361 ctaaggaaaa gacagggacc aaagcaaatg ttggttggag gagtagtgct cttgggagca
    12421 atgctggtcg ggcaagtaac tctccttgat ttgctgaaac tcacagtggc tgtgggattg
    12481 catttccatg agatgaacaa tggaggagac gccatgtata tggcgttgat tgctgccttt
    12541 tcaatcagac cagggctgct catcggcttt gggctcagga ccctatggag ccctcgggaa
    12601 cgccttgtgc tgaccctagg agcagccatg gtggagattg ccttgggtgg cgtgatgggc
    12661 ggcctgtgga agtatctaaa tgcagtttct ctctgcatcc tgacaataaa tgctgttgct
    12721 tctaggaaag catcaaatac catcttgccc ctcatggctc tgttgacacc tgtcactatg
    12781 gctgaggtga gacttgccgc aatgttcttt tgtgccatgg ttatcatagg ggtccttcac
    12841 cagaatttca aggacacctc catgcagaag actatacctc tggtggccct cacactcaca
    12901 tcttacctgg gcttgacaca accttttttg ggcctgtgtg catttctggc aacccgcata
    12961 tttgggcgaa ggagtatccc agtgaatgag gcactcgcag cagctggtct agtgggagtg
    13021 ctggcaggac tggcttttca ggagatggag aacttccttg gtccgattgc agttggagga
    13081 ctcctgatga tgctggttag cgtggctggg agggtggatg ggctagagct caagaagctt
    13141 ggtgaagttt catgggaaga ggaggcggag atcagcggga gttccgcccg ctatgatgtg
    13201 gcactcagtg aacaagggga gttcaagctg ctttctgaag agaaagtgcc atgggaccag
    13261 gttgtgatga cctcgctggc cttggttggg gctgccctcc atccatttgc tcttctgctg
    13321 gtccttgctg ggtggctgtt tcatgtcagg ggagcgcgca gaagtggtca ccggagaagt
    13381 ggggatgtct tgtgggatat tcccactcct aagatcatcg aggaatgtga acatctggag
    13441 gatgggattt atggcatatt ccagtcaacc ttcttggggg cctcccagcg aggagtggga
    13501 gtggcacagg gaggggtgtt ccacacaatg tggcatgtca caagaggagc tttccttgtc
    13561 aggaatggca agaagttgat tccatcttgg gcttcagtaa aggaagacct tgtcgcctat
    13621 ggtggctcat ggaagttgga aggcagatgg gatggagagg aagaggtcca gttgatcgcg
    13681 gctgttccag gaaagaacgt ggtcaacgtc cagacaaaac cgagcttgtt caaagtgagg
    13741 aatgggggag aaatcggggc tgtcgctctt gactatccga gtggcacttc aggatctcct
    13801 attgttaaca ggaacggaga ggtgattggg ctgtacggca atggcatcct tgtcggtgac
    13861 aactccttcg tgtccgccat atcccagact gaggtgaagg aagaaggaaa ggaggagctc
    13921 caagagatcc cgacaatgct aaagaaagga atgacaactg tccttgattt tcatcctgga
    13981 gctgggaaga caagacgttt cctcccacag atcttggccg agtgcgcacg gagacgcttg
    14041 cgcactcttg tgttggcccc caccagggtt gttctttctg aaatgaagga ggcttttcac
    14101 ggcctggacg tgaaattcca cacacaggct ttttccgctc acggcagcgg gagagaagtc
    14161 attgatgcca tgtgccatgc caccctaact tacaggatgt tggaaccaac tagggttgtt
    14221 aactgggaag tgatcattat ggatgaagcc cattttttgg atccagctag catagccgct
    14281 agaggttggg cagcgcacag agctagggca aatgaaagtg caacaatctt gatgacagcc
    14341 acaccgcctg ggactagtga tgaatttcca cattcaaatg gtgaaataga agatgttcaa
    14401 acggacatac ccagtgagcc ctggaacaca gggcatgact ggatcctggc tgacaaaagg
    14461 cccacggcat ggttccttcc atccatcaga gctgcaaatg tcatggctgc ctctttgcgt
    14521 aaggctggaa agagtgtggt ggtcctgaac aggaaaacct ttgagagaga ataccccacg
    14581 ataaagcaga agaaacctga ctttatattg gccactgaca tagctgaaat gggagccaac
    14641 ctttgcgtgg agcgagtgct ggattgcagg acggctttta agcctgtgct tgtggatgaa
    14701 gggaggaagg tggcaataaa agggccactt cgtatctccg catcctctgc tgctcaaagg
    14761 agggggcgca ttgggagaaa tcccaacaga gatggagact catactacta ttctgagcct
    14821 acaagtgaaa ataatgccca ccacgtctgc tggttggagg cctcaatgct cttggacaac
    14881 atggaggtga ggggtggaat ggtcgcccca ctctatggcg ttgaaggaac taaaacacca
    14941 gtttcccctg gtgaaatgag actgagggat gaccagagga aagtcttcag agaactagtg
    15001 aggaattgtg acctgcccgt ttggctttcg tggcaagtgg ccaaggctgg tttgaagacg
    15061 aatgatcgta agtggtgttt tgaaggccct gaggaacatg agatcttgaa tgacagcggt
    15121 gaaacagtga agtgcagggc tcctggagga gcaaagaagc ctctgcgccc aaggtggtgt
    15181 gatgaaaggg tgtcatctga ccagagtgcg ctgtctgaat ttattaagtt tgctgaaggt
    15241 aggaggggag ctgctgaagt gctagttgtg ctgagtgaac tccctgattt cctggctaaa
    15301 aaaggtggag aggcaatgga taccatcagt gtgttcctcc actctgagga aggctctagg
    15361 gcttaccgca atgcactatc aatgatgcct gaggcaatga caatagtcat gctgtttata
    15421 ctggctggac tactgacatc gggaatggtc atctttttca tgtctcccaa aggcatcagt
    15481 agaatgtcta tggcgatggg cacaatggcc ggctgtggat atctcatgtt ccttggaggc
    15541 gtcaaaccca ctcacatctc ctatgtcatg ctcatattct ttgtcctgat ggtggttgtg
    15601 atccccgagc cagggcaaca aaggtccatc caagacaacc aagtggcata cctcattatt
    15661 ggcatcctga cgctggtttc agcggtggca gccaacgagc taggcatgct ggagaaaacc
    15721 aaagaggacc tctttgggaa gaagaactta attccatcta gtgcttcacc ctggagttgg
    15781 ccggatcttg acctgaagcc aggagctgcc tggacagtgt acgttggcat tgttacaatg
    15841 ctctctccaa tgttgcacca ctggatcaaa gtcgaatatg gcaacctgtc tctgtctgga
    15901 atagcccagt cagcctcagt cctttctttc atggacaagg ggataccatt catgaagatg
    15961 aatatctcgg tcataatgct gctggtcagt ggctggaatt caataacagt gatgcctctg
    16021 ctctgtggca tagggtgcgc catgctccac tggtctctca ttttacctgg aatcaaagcg
    16081 cagcagtcaa agcttgcaca gagaagggtg ttccatggcg ttgccaagaa ccctgtggtt
    16141 gatgggaatc caacagttga cattgaggaa gctcctgaaa tgcctgccct ttatgagaag
    16201 aaactggctc tatatctcct tcttgctctc agcctagctt ctgttgccat gtgcagaacg
    16261 cccttttcat tggctgaagg cattgtccta gcatcagctg ccttagggcc gctcatagag
    16321 ggaaacacca gccttctttg gaatggaccc atggctgtct ccatgacagg agtcatgagg
    16381 gggaatcact atgcttttgt gggagtcatg tacaatctat ggaagatgaa aactggacgc
    16441 cgggggagcg cgaatggaaa aactttgggt gaagtctgga agagggaact gaatctgttg
    16501 gacaagcgac agtttgagtt gtataaaagg accgacattg tggaggtgga tcgtgatacg
    16561 gcacgcaggc atttggccga agggaaggtg gacaccgggg tggcggtctc cagggggacc
    16621 gcaaagttaa ggtggttcca tgagcgtggc tatgtcaagc tggaaggtag ggtgattgac
    16681 ctggggtgtg gccgcggagg ctggtgttac tacgctgctg cgcaaaagga agtgagtggg
    16741 gtcaaaggat ttactcttgg aagagacggc catgagaaac ccatgaatgt gcaaagtctg
    16801 ggatggaaca tcatcacctt caaggacaaa actgatatcc accgcctaga accagtgaaa
    16861 tgtgacaccc ttttgtgtga cattggagag tcatcatcgt catcggtcac agagggggaa
    16921 aggaccgtga gagttcttga tactgtagaa aaatggctgg cttgtggggt tgacaacttc
    16981 tgtgtgaagg tgttagctcc atacatgcca gatgttcttg agaaactgga attgctccaa
    17041 aggaggtttg gcggaacagt gatcaggaac cctctctcca ggaattccac tcatgaaatg
    17101 tactacgtgt ctggagcccg cagcaatgtc acatttactg tgaaccaaac atcccgcctc
    17161 ctgatgagga gaatgaggcg tccaactgga aaagtgaccc tggaggctga cgtcatcctc
    17221 ccaattggga cacgcagtgt tgagacagac aagggacccc tggacaaaga ggccatagaa
    17281 gaaagggttg agaggataaa atctgagtac atgacctctt ggttttatga caatgacaac
    17341 ccctacagga cctggcacta ctgtggctcc tatgtcacaa aaacctcagg aagtgcggcg
    17401 agcatggtaa atggtgttat taaaattctg acatatccat gggacaggat agaggaggtc
    17461 acaagaatgg caatgactga cacaacccct tttggacagc aaagagtgtt taaagaaaaa
    17521 gttgacacca gagcaaagga tccaccagcg ggaactagga agatcatgaa agttgtcaac
    17581 aggtggctgt tccgccacct ggccagagaa aagaacccca gactgtgcac aaaggaagaa
    17641 tttattgcaa aagtccgaag tcatgcagcc attggagctt acctggaaga acaagaacag
    17701 tggaagactg ccaatgaggc tgtccaagac ccaaagttct gggaactggt ggatgaagaa
    17761 aggaagctgc accaacaagg caggtgtcgg acttgtgtgt acaacatgat ggggaaaaga
    17821 gagaagaagc tgtcagagtt tgggaaagca aagggaagcc gtgccatatg gtatatgtgg
    17881 ctgggagcgc ggtatcttga gtttgaggcc ctgggattcc tgaatgagga ccattgggct
    17941 tccagggaaa actcaggagg aggagtggaa ggcattggct tacaatacct aggatatgtg
    18001 atcagagacc tggctgcaat ggatggtggt ggattctacg cggatgacac cgctggatgg
    18061 gacacgcgca tcacagaggc agaccttgat gatgaacagg agatcttgaa ctacatgagc
    18121 ccacatcaca aaaaactggc acaagcagtg atggaaatga catacaagaa caaagtggtg
    18181 aaagtgttga gaccagcccc aggagggaaa gcctacatgg atgtcataag tcgacgagac
    18241 cagagaggat ccgggcaggt agtgacttat gctctgaaca ccatcaccaa cttgaaagtc
    18301 caattgatca gaatggcaga agcagagatg gtgatacatc accaacatgt tcaagattgt
    18361 gatgaatcag ttctgaccag gctggaggca tggctcactg agcacggatg tgacagactg
    18421 aagaggatgg cggtgagtgg agacgactgt gtggtccggc ccatcgatga caggttcggc
    18481 ctggccctgt cccatctcaa cgccatgtcc aaggttagaa aggacatatc tgaatggcag
    18541 ccatcaaaag ggtggaatga ttgggagaat gtgcccttct gttcccacca cttccatgaa
    18601 ctacagctga aggatggcag gaggattgtg gtgccttgcc gagaacagga cgagctcatt
    18661 gggagaggaa gggtgtctcc aggaaacggc tggatgatca aggaaacagc ttgcctcagc
    18721 aaagcctatg ccaacatgtg gtcactgatg tattttcaca aaagggacat gaggctactg
    18781 tcattggctg tttcctcagc tgttcccacc tcatgggttc cacaaggacg cacaacatgg
    18841 tcgattcatg ggaaagggga gtggatgacc acggaagaca tgcttgaggt gtggaacaga
    18901 gtatggataa ccaacaaccc acacatgcag gacaagacaa tggtgaaaaa atggagagat
    18961 gtcccttatc taaccaagag acaagacaag ctgtgcggat cactgattgg aatgaccaat
    19021 agggccacct gggcctccca catccattta gtcatccatc gtatccgaac gctgattgga
    19081 caggagaaat acactgacta cctaacagtc atggacaggt attctgtgga tgctgacctg
    19141 caactgggtg agcttatctg aaacaccatc taacaggaat aaccgggata caaaccacgg
    19201 gtggagaacc ggactcccca caacctgaaa ccgggatata aaccacggct ggagaaccgg
    19261 actccgcact taaaatgaaa cagaaaccgg gataaaaact acggatggag aaccggactc
    19321 cacacattga gacagaagaa gttgtcagcc cagaacccca cacgagtttt gccactgcta
    19381 agctgtgagg cagtgcaggc tgggacagcc gacctccagg ttgcgaaaaa cctggtttct
    19441 gggacctccc accccagagt aaaaagaacg gagcctccgc taccaccctc ccacgtggtg
    19501 gtagaaagac ggggtctaga ggttagagga gaccctccag ggaacaaata gtgggaccat
    19561 attgacgcca gggaaagacc ggagtggttc tctgcttttc ctccagaggt ctgtgagcac
    19621 agtttgctca agaataagca gacctttgga tgacaaacac aaaaccactc gagcaagacg
    19681 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
    19741 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
    19801 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
    19861 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
    19921 cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga
    19981 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctagcgga
    20041 gtgtagtggg atccccgggt accgagctcg aattcgccct atagtgagtc gtattacaat
    20101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    20161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    20221 cgcccttccc aacagttgcg cagctgaatg gcgaatggcg cctgatgcgg tattttctcc
    20281 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
    20341 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg aaccccttgc
    20401 ggcc
//
