LOCUS       sequence-354333-       20728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) 
            but with Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20728)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 20728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelNS1-Cre
FEATURES             Location/Qualifiers
     source          1..20728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..43
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    61..459
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    719..1192
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1265..2236)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2236..3411)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3737..3987
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3990..4745)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4836..5055
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5123..5654
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5131..5745
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6603..6705
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6706..7365
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6772..6791)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7585..7606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7621..7626,7627..7644,7645..7651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7664..7686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7683..7699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7683..7699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8136..8154
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8136..8153
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             8353..9384
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSG
                     LPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLA
                     FLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLV
                     ERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSG
                     QRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLL
                     EDGD"
     primer_bind     20018..20037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(20401..20420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(20402..20420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(20427..20444)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(20427..20443)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20436..20458)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(20651..20670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatcgaata taacttcgta taatgtatgc tatacgaagt tattagcgat gagctcggac
       61 ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagctc
      121 gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
      181 tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
      241 gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
      301 ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
      361 tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaagcaa cttcagacaa
      421 tacaaatcag cgacactgaa tacggggcaa cctcatgtcc gagctcgcga gctcgtcgac
      481 agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag
      541 gtattttgtc cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc
      601 cacagcaggc atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt
      661 caatacttgc ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga
      721 caatacaagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg
      781 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
      841 tcagaccgac gatacgagtg ggaccgtggt tccagactaa taatcagacc gacgatacga
      901 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac catggtccca
      961 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     1021 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     1081 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     1141 atcagaccga cgatacaagt ggaacagtgg gcccagagag aatattcagg ccagttatgc
     1201 tttctggcct gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt
     1261 cgcatcaggg tgctggcttt tcaagttcct taagaatggc ctcaattttc tctatacact
     1321 cagttggaac acgagacctg tccaggttaa gcaccatttt atcgccctta tacaatactg
     1381 tcgctccagg agcaaactga tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt
     1441 taagcacaga agttaaaaga gtgataactt cttcagcttc aaatatcacc ccagcttttt
     1501 tctgctcatg aaggttagat gcctgctgct taagtaattc ctctttatct gtaaaggctt
     1561 tttgaagtgc atcacctgac cgggcagata gttcaccggg gtgagaaaaa agagcaacaa
     1621 ctgatttagg caatttggcg gtgttgatac agcgggtaat aatcttacgt gaaatatttt
     1681 ccgcatcagc cagcgcagaa atatttccag caaattcatt ctgcaatcgg cttgcataac
     1741 gctgaccacg ttcataagca cttgttgggc gataatcgtt acccaatctg gataatgcag
     1801 ccatctgctc atcatccagc tcgccaacca gaacacgata atcactttcg gtaagtgcag
     1861 cagctttacg acggcgactc ccatcggcaa tttctatgac accagatact cttcgaccga
     1921 acgccggtgt ctgttgacca gtcagtagaa aagaagggat gagatcatcc agtgcgtcct
     1981 cagtaagcag ctcctggtca cgttcattac ctgaccatac ccgagaggtc ttctcaacac
     2041 tatcaccccg gagcacttca agagtaaact tcacatcccg accacataca ggcaaagtaa
     2101 tggcattacc gcgagccatt actcctacgc gcgcaattaa cgaatccacc atcggggcag
     2161 ctggtgtcga taacgaagta tcttcaaccg gttgagtatt gagcgtatgt tttggaataa
     2221 caggcgcacg cttcattatc taatctccca gcgtggttta atcagacgat cgaaaatttc
     2281 attgcagaca ggttcccaaa tagaaagagc atttctccag gcaccagttg aagagcgttg
     2341 atcaatggcc tgttcaaaaa cagttctcat ccggatctga cctttaccaa cttcatccgt
     2401 ttcacgtaca acatttttta gaaccatgct tccccaggca tcccgaattt gctcctccat
     2461 ccacggggac tgagagccat tactattgct gtatttggta agcaaaatac gtacatcagg
     2521 ctcgaaccct ttaagatcaa cgttcttgag cagatcacga agcatatcga aaaactgcag
     2581 tgcggaggtg tagtcaaaca actcagcagg cgtgggaaca atcagcacat cagcagcaca
     2641 tacgacatta atcgtgccga tacccaggtt aggcgcgctg tcaataacta tgacatcata
     2701 gtcatgagca acagtttcaa tggccagtcg gagcatcagg tgtggatcgg tgggcagttt
     2761 accttcatca aatttgccca ttaactcagt ttcaatacgg tgcagagcca gacaggaagg
     2821 aataatgtca agccccggcc agcaagtggg ctttattgca taagtgacat cgtccttttc
     2881 cccaagatag aaaggcagga gagtgtcttc tgcatgaata tgaagatctg gtacccatcc
     2941 gtgatacatt gaggctgttc cctgggggtc gttaccttcc acgagcaaaa cacgtagccc
     3001 cttcagagcc agatcctgag caagatgaac agaaactgag gttttgtaaa cgccaccttt
     3061 atgggcagca accccgatca ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc
     3121 aaacacatca cgcatatgat taatttgttc aattgtataa ccaacacgtt gctcaacccg
     3181 tcctcgaatt tccatatccg ggtgcggtag tcgccctgct ttctcggcat ctctgatagc
     3241 ctgagaagaa accccaacta aatccgctgc ttcacctatt ctccagcgcc gggttatttt
     3301 cctcgcttcc gggctgtcat cattaaactg tgcaatggcg atagccttcg tcatttcatg
     3361 accagcgttt atgcactggt taagtgtttc catgagtttc attctgaaca tcctttaatc
     3421 attgctttgc gtttttttat taaatcttgc aatttactgc aaagcaacaa caaaatcgca
     3481 aagtcatcaa aaaaccgcaa agttgtttaa aataagagca acactacaaa aggagataag
     3541 aagagcacat acctcagtca cttattatca ctagcgctcg ccgcagccgt gtaaccgagc
     3601 atagcgagcg aactggcgag gaagcaaaga agaactgttc tgtcagatag ctcttacgct
     3661 cagcgcaaga agaaatatcc accgtgggaa aaactccagg tagaggtaca cacgcggata
     3721 gccaattcag agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc
     3781 cgatatcaag tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc
     3841 aaatttggct tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa
     3901 ggaaacagca aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac
     3961 cctcaaatct gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg
     4021 aaggaaaata cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc
     4081 attggagttc tgctgttgat ctcattaaca cagacctgca ggaagcggcg gcggaagtca
     4141 ggcatacgct ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga
     4201 gagacgatgc ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg
     4261 gcatacggat tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt
     4321 aacccgataa agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat
     4381 ggactgtgcg cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca
     4441 tcctcttcag ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt
     4501 atatccttac tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg
     4561 atctcgcaga taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg
     4621 aacagataca gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg
     4681 tttgactgga cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc
     4741 gccatgacag atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag
     4801 agtcaataag cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca
     4861 gggttaaggg caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa
     4921 agggcaattt gtcacaacac cttctctaga accagcatgg ataaaggcct acaaggcgct
     4981 ctaaaaaaga agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggataa
     5041 gtggataacc ccaagggaag ttttttcagg catcgtgtgt aagcagaata tataagtgct
     5101 gttccctggt gcttcctcgc tcactcgacc gggagggttc gagaaggggg ggcacccccc
     5161 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     5221 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     5281 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     5341 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     5401 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     5461 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     5521 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     5581 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     5641 caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     5701 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagccgag ggcttcgccc
     5761 tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat catggttctg
     5821 tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa cgtaacaccg
     5881 cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg
     5941 catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat taataatgcg
     6001 gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag tggataacag
     6061 ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca tttccatata
     6121 aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca tcagactccg
     6181 gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga
     6241 cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc agcggtatac
     6301 cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg accggaacag
     6361 agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag gtcctgaccg
     6421 ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga cggttacgcc
     6481 gctccatgag cttatcgcga ataaatacct gtgacggaag atcacttcgc agaataaata
     6541 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     6601 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     6661 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     6721 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     6781 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     6841 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     6901 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     6961 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7021 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7081 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7141 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7201 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7261 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     7321 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     7381 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     7441 ggcagaaatt cgatgataag ctgtcaaaca tgagaattgg tcgacggccc gggcggccgc
     7501 aaggagttcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7561 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7621 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7681 cacaggaaac agctatgacc atgattacgc caagctattt aggtgagact atagaatact
     7741 caagcttgca tgcctgcagg tcgactctag aggatcccac attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtgt cgacgcggcc gctagcgatg
     7921 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     7981 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
     8041 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
     8101 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagagtaaat
     8161 cctgtgtgct aattgaggtg cattggtctg caaatcgagt tgctaggcaa taaacacatt
     8221 tggattaatt ttaatcgttc gttgagcgat tagcagagaa ctgaccagaa catgtctggt
     8281 cgtaaagctc agggaaaaac cctgggcgtc aatatggtac gacgaggagt tcgctccttg
     8341 tcaaacgagc tcatggtgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
     8401 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
     8461 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
     8521 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     8581 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     8641 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     8701 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     8761 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     8821 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     8881 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     8941 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     9001 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     9061 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     9121 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     9181 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     9241 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     9301 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     9361 gtgcgcctgc tggaagatgg cgataccggt aactttgacc ttctcaagtt ggccggcgac
     9421 gtcgagtcca acccagggcc cctgcagcaa attttcgtga agaccctgac gggcaagacc
     9481 atcactcttg aggtcgagcc cagtgacacc atcgagaatg tcaaggccaa gatccaagac
     9541 aaggaaggca tcccacctga ccagcagagg ctgatattcg cgggcaaaca gctggaggat
     9601 ggccgcaccc tgtccgacta caacatccag aaagagtcca ccttgcacct ggtgctgtgt
     9661 ctccgcggtg gaatgtccgg tcgtaaagcc cagggcaaaa ccctaggcgt aaatatggtc
     9721 cgacgcggag tccgctcatt gtcgaacaaa ataaaacaaa aaacaaaaca aattggaaac
     9781 agacctggac cttcaagagg tgttcaagga tttatctttt tctttttgtt caacattttg
     9841 actggaaaaa agatcacagc ccacctaaag aggttgtgga aaatgctgga cccaagacaa
     9901 ggcttggctg ttctaaggaa agtcaagaga gtggtggcca gtttgatgag aggattgtcc
     9961 tcaaggaaac gccgttccca tgatgttctg actgtgcaat tcctaatttt gggaatgctg
    10021 ttgatgacgg gtggagtgac cttggtgcgg aaaaacagat ggttgctcct aaatgtgaca
    10081 tctgaggacc tcgggaaaac attctctgtg ggcacaggca actgcacaac aaacattttg
    10141 gaagccaagt actggtgccc agactcaatg gaatacaact gtcccaatct cagtccaaga
    10201 gaggagccag atgacattga ttgctggtgc tatggggtgg aaaacgttag agtcgcatat
    10261 ggtaagtgtg actcagcagg caggtctagg aggtcaagaa gggccattga cttgcctacg
    10321 catgaaaacc atggtttgaa gacccggcaa gaaaaatgga tgactggaag aatgggtgaa
    10381 aggcaactcc aaaagattga gagatggttc gtgaggaacc ccttttttgc agtgacggct
    10441 ctgaccattg cctaccttgt gggaagcaac atgacgcaac gagtcgtgat tgccctactg
    10501 gtcttggctg ttggtccggc ctactcagct cactgcattg gaattactga cagggatttc
    10561 attgaggggg tgcatggagg aacttgggtt tcagctaccc tggagcaaga caagtgtgtc
    10621 actgttatgg cccctgacaa gccttcattg gacatctcac tagagacagt agccattgat
    10681 agacctgctg aggtgaggaa agtgtgttac aatgcagttc tcactcatgt gaagattaat
    10741 gacaagtgcc ccagcactgg agaggcccac ctagccgaag agaacgaagg ggacaatgcg
    10801 tgcaagcgca cttattctga tagaggctgg ggcaatggct gtggcctatt tgggaaaggg
    10861 agcattgtgg catgcgccaa attcacttgt gccaaatcca tgagtttgtt tgaggttgat
    10921 cagaccaaaa ttcagtatgt catcagagca caattgcatg taggggccaa gcaggaaaat
    10981 tggaataccg acattaagac tctcaagttt gatgccctgt caggctccca ggaagtcgag
    11041 ttcattgggt atggaaaagc tacactggaa tgccaggtgc aaactgcggt ggactttggt
    11101 aacagttaca tcgctgagat ggaaacagag agctggatag tggacagaca gtgggcccag
    11161 gacttgaccc tgccatggca gagtggaagt ggcggggtgt ggagagagat gcatcatctt
    11221 gtcgaatttg aacctccgca tgccgccact atcagagtac tggccctggg aaaccaggaa
    11281 ggctccttga aaacagctct tactggcgca atgagggtta caaaggacac aaatgacaac
    11341 aacctttaca aactacatgg tggacatgtt tcttgcagag tgaaattgtc agctttgaca
    11401 ctcaagggga catcctacaa aatatgcact gacaaaatgt tttttgtcaa gaacccaact
    11461 gacactggcc atggcactgt tgtgatgcag gtgaaagtgt caaaaggagc cccctgcagg
    11521 attccagtga tagtagctga tgatcttaca gcggcaatca ataaaggcat tttggttaca
    11581 gttaacccca tcgcctcaac caatgatgat gaagtgctga ttgaggtgaa cccacctttt
    11641 ggagacagct acattatcgt tgggagagga gattcacgtc tcacttacca gtggcacaaa
    11701 gagggaagct caataggaaa gttgttcact cagaccatga aaggcgtgga acgcctggcc
    11761 gtcatgggag acaccgcctg ggatttcagc tccgctggag ggttcttcac ttcggttggg
    11821 aaaggaattc atacggtgtt tggctctgcc tttcaggggc tatttggcgg cttgaactgg
    11881 ataacaaagg tcatcatggg ggcggtactt atatgggttg gcatcaacac aagaaacatg
    11941 acaatgtcca tgagcatgat cttggtagga gtgatcatga tgtttttgtc tctaggagtt
    12001 ggggcggatc aaggatgcgc catcaacttt ggcaagagag agctcggatc acggtccggg
    12061 acacgcgtgt acatggacgc agtctttgaa tacaccatag actgcgatgg atctatcttg
    12121 ggtgcagcgg tgaacggaaa aaagagtgcc catggctctc caacattttg gatgggaagt
    12181 catgaagtaa atgggacatg gatgatccac accttggagg cattagatta caaggagtgt
    12241 gagtggccac tgacacatac gattggaaca tcagttgaag agagtgaaat gttcatgccg
    12301 agatcaatcg gaggcccagt tagctctcac aatcatatcc ctggatacaa ggttcagacg
    12361 aacggacctt ggatgcaggt accactagaa gtgaagagag aagcttgccc agggactagc
    12421 gtgatcattg atggcaactg tgatggacgg ggaaaatcaa ccagatccac cacggatagc
    12481 gggaaagtta ttcctgaatg gtgttgccgc tcctgcacaa tgccgcctgt gagcttccat
    12541 ggtagtgatg ggtgttggta tcccatggaa attaggccaa ggaaaacgca tgaaagccat
    12601 ctggtgcgct cctgggttac agctggagaa atacatgctg tcccttttgg tttggtgagc
    12661 atgatgatag caatggaagt ggtcctaagg aaaagacagg gaccaaagca aatgttggtt
    12721 ggaggagtag tgctcttggg agcaatgctg gtcgggcaag taactctcct tgatttgctg
    12781 aaactcacag tggctgtggg attgcatttc catgagatga acaatggagg agacgccatg
    12841 tatatggcgt tgattgctgc cttttcaatc agaccagggc tgctcatcgg ctttgggctc
    12901 aggaccctat ggagccctcg ggaacgcctt gtgctgaccc taggagcagc catggtggag
    12961 attgccttgg gtggcgtgat gggcggcctg tggaagtatc taaatgcagt ttctctctgc
    13021 atcctgacaa taaatgctgt tgcttctagg aaagcatcaa ataccatctt gcccctcatg
    13081 gctctgttga cacctgtcac tatggctgag gtgagacttg ccgcaatgtt cttttgtgcc
    13141 atggttatca taggggtcct tcaccagaat ttcaaggaca cctccatgca gaagactata
    13201 cctctggtgg ccctcacact cacatcttac ctgggcttga cacaaccttt tttgggcctg
    13261 tgtgcatttc tggcaacccg catatttggg cgaaggagta tcccagtgaa tgaggcactc
    13321 gcagcagctg gtctagtggg agtgctggca ggactggctt ttcaggagat ggagaacttc
    13381 cttggtccga ttgcagttgg aggactcctg atgatgctgg ttagcgtggc tgggagggtg
    13441 gatgggctag agctcaagaa gcttggtgaa gtttcatggg aagaggaggc ggagatcagc
    13501 gggagttccg cccgctatga tgtggcactc agtgaacaag gggagttcaa gctgctttct
    13561 gaagagaaag tgccatggga ccaggttgtg atgacctcgc tggccttggt tggggctgcc
    13621 ctccatccat ttgctcttct gctggtcctt gctgggtggc tgtttcatgt caggggagcg
    13681 cgcagaagtg gtcaccggag aagtggggat gtcttgtggg atattcccac tcctaagatc
    13741 atcgaggaat gtgaacatct ggaggatggg atttatggca tattccagtc aaccttcttg
    13801 ggggcctccc agcgaggagt gggagtggca cagggagggg tgttccacac aatgtggcat
    13861 gtcacaagag gagctttcct tgtcaggaat ggcaagaagt tgattccatc ttgggcttca
    13921 gtaaaggaag accttgtcgc ctatggtggc tcatggaagt tggaaggcag atgggatgga
    13981 gaggaagagg tccagttgat cgcggctgtt ccaggaaaga acgtggtcaa cgtccagaca
    14041 aaaccgagct tgttcaaagt gaggaatggg ggagaaatcg gggctgtcgc tcttgactat
    14101 ccgagtggca cttcaggatc tcctattgtt aacaggaacg gagaggtgat tgggctgtac
    14161 ggcaatggca tccttgtcgg tgacaactcc ttcgtgtccg ccatatccca gactgaggtg
    14221 aaggaagaag gaaaggagga gctccaagag atcccgacaa tgctaaagaa aggaatgaca
    14281 actgtccttg attttcatcc tggagctggg aagacaagac gtttcctccc acagatcttg
    14341 gccgagtgcg cacggagacg cttgcgcact cttgtgttgg cccccaccag ggttgttctt
    14401 tctgaaatga aggaggcttt tcacggcctg gacgtgaaat tccacacaca ggctttttcc
    14461 gctcacggca gcgggagaga agtcattgat gccatgtgcc atgccaccct aacttacagg
    14521 atgttggaac caactagggt tgttaactgg gaagtgatca ttatggatga agcccatttt
    14581 ttggatccag ctagcatagc cgctagaggt tgggcagcgc acagagctag ggcaaatgaa
    14641 agtgcaacaa tcttgatgac agccacaccg cctgggacta gtgatgaatt tccacattca
    14701 aatggtgaaa tagaagatgt tcaaacggac atacccagtg agccctggaa cacagggcat
    14761 gactggatcc tggctgacaa aaggcccacg gcatggttcc ttccatccat cagagctgca
    14821 aatgtcatgg ctgcctcttt gcgtaaggct ggaaagagtg tggtggtcct gaacaggaaa
    14881 acctttgaga gagaataccc cacgataaag cagaagaaac ctgactttat attggccact
    14941 gacatagctg aaatgggagc caacctttgc gtggagcgag tgctggattg caggacggct
    15001 tttaagcctg tgcttgtgga tgaagggagg aaggtggcaa taaaagggcc acttcgtatc
    15061 tccgcatcct ctgctgctca aaggaggggg cgcattggga gaaatcccaa cagagatgga
    15121 gactcatact actattctga gcctacaagt gaaaataatg cccaccacgt ctgctggttg
    15181 gaggcctcaa tgctcttgga caacatggag gtgaggggtg gaatggtcgc cccactctat
    15241 ggcgttgaag gaactaaaac accagtttcc cctggtgaaa tgagactgag ggatgaccag
    15301 aggaaagtct tcagagaact agtgaggaat tgtgacctgc ccgtttggct ttcgtggcaa
    15361 gtggccaagg ctggtttgaa gacgaatgat cgtaagtggt gttttgaagg ccctgaggaa
    15421 catgagatct tgaatgacag cggtgaaaca gtgaagtgca gggctcctgg aggagcaaag
    15481 aagcctctgc gcccaaggtg gtgtgatgaa agggtgtcat ctgaccagag tgcgctgtct
    15541 gaatttatta agtttgctga aggtaggagg ggagctgctg aagtgctagt tgtgctgagt
    15601 gaactccctg atttcctggc taaaaaaggt ggagaggcaa tggataccat cagtgtgttc
    15661 ctccactctg aggaaggctc tagggcttac cgcaatgcac tatcaatgat gcctgaggca
    15721 atgacaatag tcatgctgtt tatactggct ggactactga catcgggaat ggtcatcttt
    15781 ttcatgtctc ccaaaggcat cagtagaatg tctatggcga tgggcacaat ggccggctgt
    15841 ggatatctca tgttccttgg aggcgtcaaa cccactcaca tctcctatgt catgctcata
    15901 ttctttgtcc tgatggtggt tgtgatcccc gagccagggc aacaaaggtc catccaagac
    15961 aaccaagtgg catacctcat tattggcatc ctgacgctgg tttcagcggt ggcagccaac
    16021 gagctaggca tgctggagaa aaccaaagag gacctctttg ggaagaagaa cttaattcca
    16081 tctagtgctt caccctggag ttggccggat cttgacctga agccaggagc tgcctggaca
    16141 gtgtacgttg gcattgttac aatgctctct ccaatgttgc accactggat caaagtcgaa
    16201 tatggcaacc tgtctctgtc tggaatagcc cagtcagcct cagtcctttc tttcatggac
    16261 aaggggatac cattcatgaa gatgaatatc tcggtcataa tgctgctggt cagtggctgg
    16321 aattcaataa cagtgatgcc tctgctctgt ggcatagggt gcgccatgct ccactggtct
    16381 ctcattttac ctggaatcaa agcgcagcag tcaaagcttg cacagagaag ggtgttccat
    16441 ggcgttgcca agaaccctgt ggttgatggg aatccaacag ttgacattga ggaagctcct
    16501 gaaatgcctg ccctttatga gaagaaactg gctctatatc tccttcttgc tctcagccta
    16561 gcttctgttg ccatgtgcag aacgcccttt tcattggctg aaggcattgt cctagcatca
    16621 gctgccttag ggccgctcat agagggaaac accagccttc tttggaatgg acccatggct
    16681 gtctccatga caggagtcat gagggggaat cactatgctt ttgtgggagt catgtacaat
    16741 ctatggaaga tgaaaactgg acgccggggg agcgcgaatg gaaaaacttt gggtgaagtc
    16801 tggaagaggg aactgaatct gttggacaag cgacagtttg agttgtataa aaggaccgac
    16861 attgtggagg tggatcgtga tacggcacgc aggcatttgg ccgaagggaa ggtggacacc
    16921 ggggtggcgg tctccagggg gaccgcaaag ttaaggtggt tccatgagcg tggctatgtc
    16981 aagctggaag gtagggtgat tgacctgggg tgtggccgcg gaggctggtg ttactacgct
    17041 gctgcgcaaa aggaagtgag tggggtcaaa ggatttactc ttggaagaga cggccatgag
    17101 aaacccatga atgtgcaaag tctgggatgg aacatcatca ccttcaagga caaaactgat
    17161 atccaccgcc tagaaccagt gaaatgtgac acccttttgt gtgacattgg agagtcatca
    17221 tcgtcatcgg tcacagaggg ggaaaggacc gtgagagttc ttgatactgt agaaaaatgg
    17281 ctggcttgtg gggttgacaa cttctgtgtg aaggtgttag ctccatacat gccagatgtt
    17341 cttgagaaac tggaattgct ccaaaggagg tttggcggaa cagtgatcag gaaccctctc
    17401 tccaggaatt ccactcatga aatgtactac gtgtctggag cccgcagcaa tgtcacattt
    17461 actgtgaacc aaacatcccg cctcctgatg aggagaatga ggcgtccaac tggaaaagtg
    17521 accctggagg ctgacgtcat cctcccaatt gggacacgca gtgttgagac agacaaggga
    17581 cccctggaca aagaggccat agaagaaagg gttgagagga taaaatctga gtacatgacc
    17641 tcttggtttt atgacaatga caacccctac aggacctggc actactgtgg ctcctatgtc
    17701 acaaaaacct caggaagtgc ggcgagcatg gtaaatggtg ttattaaaat tctgacatat
    17761 ccatgggaca ggatagagga ggtcacaaga atggcaatga ctgacacaac cccttttgga
    17821 cagcaaagag tgtttaaaga aaaagttgac accagagcaa aggatccacc agcgggaact
    17881 aggaagatca tgaaagttgt caacaggtgg ctgttccgcc acctggccag agaaaagaac
    17941 cccagactgt gcacaaagga agaatttatt gcaaaagtcc gaagtcatgc agccattgga
    18001 gcttacctgg aagaacaaga acagtggaag actgccaatg aggctgtcca agacccaaag
    18061 ttctgggaac tggtggatga agaaaggaag ctgcaccaac aaggcaggtg tcggacttgt
    18121 gtgtacaaca tgatggggaa aagagagaag aagctgtcag agtttgggaa agcaaaggga
    18181 agccgtgcca tatggtatat gtggctggga gcgcggtatc ttgagtttga ggccctggga
    18241 ttcctgaatg aggaccattg ggcttccagg gaaaactcag gaggaggagt ggaaggcatt
    18301 ggcttacaat acctaggata tgtgatcaga gacctggctg caatggatgg tggtggattc
    18361 tacgcggatg acaccgctgg atgggacacg cgcatcacag aggcagacct tgatgatgaa
    18421 caggagatct tgaactacat gagcccacat cacaaaaaac tggcacaagc agtgatggaa
    18481 atgacataca agaacaaagt ggtgaaagtg ttgagaccag ccccaggagg gaaagcctac
    18541 atggatgtca taagtcgacg agaccagaga ggatccgggc aggtagtgac ttatgctctg
    18601 aacaccatca ccaacttgaa agtccaattg atcagaatgg cagaagcaga gatggtgata
    18661 catcaccaac atgttcaaga ttgtgatgaa tcagttctga ccaggctgga ggcatggctc
    18721 actgagcacg gatgtgacag actgaagagg atggcggtga gtggagacga ctgtgtggtc
    18781 cggcccatcg atgacaggtt cggcctggcc ctgtcccatc tcaacgccat gtccaaggtt
    18841 agaaaggaca tatctgaatg gcagccatca aaagggtgga atgattggga gaatgtgccc
    18901 ttctgttccc accacttcca tgaactacag ctgaaggatg gcaggaggat tgtggtgcct
    18961 tgccgagaac aggacgagct cattgggaga ggaagggtgt ctccaggaaa cggctggatg
    19021 atcaaggaaa cagcttgcct cagcaaagcc tatgccaaca tgtggtcact gatgtatttt
    19081 cacaaaaggg acatgaggct actgtcattg gctgtttcct cagctgttcc cacctcatgg
    19141 gttccacaag gacgcacaac atggtcgatt catgggaaag gggagtggat gaccacggaa
    19201 gacatgcttg aggtgtggaa cagagtatgg ataaccaaca acccacacat gcaggacaag
    19261 acaatggtga aaaaatggag agatgtccct tatctaacca agagacaaga caagctgtgc
    19321 ggatcactga ttggaatgac caatagggcc acctgggcct cccacatcca tttagtcatc
    19381 catcgtatcc gaacgctgat tggacaggag aaatacactg actacctaac agtcatggac
    19441 aggtattctg tggatgctga cctgcaactg ggtgagctta tctgaaacac catctaacag
    19501 gaataaccgg gatacaaacc acgggtggag aaccggactc cccacaacct gaaaccggga
    19561 tataaaccac ggctggagaa ccggactccg cacttaaaat gaaacagaaa ccgggataaa
    19621 aactacggat ggagaaccgg actccacaca ttgagacaga agaagttgtc agcccagaac
    19681 cccacacgag ttttgccact gctaagctgt gaggcagtgc aggctgggac agccgacctc
    19741 caggttgcga aaaacctggt ttctgggacc tcccacccca gagtaaaaag aacggagcct
    19801 ccgctaccac cctcccacgt ggtggtagaa agacggggtc tagaggttag aggagaccct
    19861 ccagggaaca aatagtggga ccatattgac gccagggaaa gaccggagtg gttctctgct
    19921 tttcctccag aggtctgtga gcacagtttg ctcaagaata agcagacctt tggatgacaa
    19981 acacaaaacc actcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
    20041 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
    20101 tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg
    20161 agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa
    20221 agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca
    20281 ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac
    20341 gaggcagacc tcagcgctag cggagtgtag tgggatcccc gggtaccgag ctcgaattcg
    20401 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    20461 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    20521 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagctg aatggcgaat
    20581 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    20641 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
    20701 cacccgctga cgcgaacccc ttgcggcc
//
