LOCUS       sequence-354340-        8585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of catalytic dead hMOV10 K530A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
  TITLE     Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts 
            retrotransposition.
  JOURNAL   EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
  PUBMED    35856394
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 20:e54458. doi: 10.15252/embr.202154458."
COMMENT     Sequence Label: pcDNA3-T11HA-hMOV10-CD
FEATURES             Location/Qualifiers
     source          1..8585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             979..1005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    3688..3699
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        complement(4138..4156)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4139..4156)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4176..4193)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4182..4406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4452..4880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4539..4558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4749..4770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4889..4909)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4894..5223
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5074..5209
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5136..5155
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5290..6084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5344..5363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5954..5973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6258..6379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6295..6314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6349..6368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6428..6444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6428..6444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6441..6463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6452..6468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6476..6482,6483..6500,6501..6506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6521..6542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6659..6676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6830..7418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6910..6929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7589..8380,8381..8449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8212..8231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8450..8554)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg
      901 gagaagaggg accacatggt gctgctggag tacgtgaccg ccgccggcat caccgacgcc
      961 tcgggggtac caggttcata tccgtatgat gtgccggatt atgcgggcgg acccgggagc
     1021 ggcggtgagg gctcagccgg cggaggaccg gtcggaggcg gatccatgcc cagtaagttc
     1081 agctgccggc agctccggga ggcgggccag tgtttcgaga gtttcctggt cgttcgggga
     1141 ctggacatgg agacagatcg cgagcggctg cggaccattt ataaccgcga cttcaagatc
     1201 agctttggga cccccgcccc tggcttctcc tccatgctgt atggaatgaa gattgcaaat
     1261 ctggcctacg tcaccaagac tcgggtcagg ttcttcagac tcgaccgctg ggccgacgtg
     1321 cggttcccag aaaagaggag aatgaagctg gggtcagata tcagcaaaca ccacaagtca
     1381 ctgctagcca agatctttta tgacagggct gagtatcttc atgggaaaca tggtgtggat
     1441 gtggaagtcc aggggcccca tgaagcccga gatgggcagc tccttatccg cctggatttg
     1501 aaccgcaaag aggtgctgac cctgaggctt cggaatggcg gaacccagtc tgttaccctc
     1561 actcacctct tcccactctg ccggacaccc cagtttgctt tctacaatga agaccaggag
     1621 ttgccctgtc cactgggccc cggtgaatgc tatgaactcc atgtccattg taagaccagc
     1681 tttgtgggct acttcccagc cacagtgctc tgggagctgc tgggacctgg ggagtcgggt
     1741 tcagaaggag ccggcacatt ctacattgcc cgcttcttgg ctgccgtcgc ccacagcccc
     1801 ctggctgcac agctgaagcc catgactccc ttcaagcgga cccggatcac cggaaaccct
     1861 gtggtgacca atcggataga ggaaggagag agacctgacc gcgctaaggg ctatgacctg
     1921 gagttaagta tggcgctggg gacatactac ccacctcccc gcctcaggca gctgctcccc
     1981 atgcttcttc agggaacaag tatcttcact gcccctaagg agatcgcaga gatcaaggcc
     2041 cagctggaga cagccctgaa gtggaggaac tatgaggtga agctgcggct gctgctgcac
     2101 ctggaggaac tgcagatgga gcatgatatc cggcactatg acctggagtc ggtgcccatg
     2161 acctgggacc ctgtggacca gaaccccagg ctgctcacgc tggaggttcc tggagtgact
     2221 gagagccgcc cctcagtgct acggggcgac cacctgtttg cccttttgtc ctcggagaca
     2281 caccaggagg accccatcac atataagggc tttgtgcaca aggtggaatt ggaccgtgtc
     2341 aagctgagct tttccatgag cctcctgagc cgctttgtgg atgggctgac cttcaaggtg
     2401 aactttacct tcaaccgcca gccgctgcga gtccagcacc gtgccctgga gctgacaggg
     2461 cgctggctgc tgtggcccat gctctttcct gtggcacctc gggacgtccc gctgctgccc
     2521 tcagatgtga aactcaagct gtacgaccgg agtctggagt caaacccaga gcagctgcag
     2581 gccatgaggc acattgttac gggcaccacc cgtccagccc cctacatcat ctttgggcct
     2641 ccaggcaccg gcgcaactgt cacgttagtg gaggcaatta agcaggtggt gaagcacttg
     2701 cccaaagccc acatcttggc ctgcgctcca tccaactcag gggctgacct actctgtcaa
     2761 aggctccggg tccaccttcc tagctccatc taccgcctcc tggcccccag cagggacatc
     2821 cgcatggtac ctgaggacat caagccctgc tgcaactggg acgcaaagaa gggggagtat
     2881 gtatttcccg ccaagaagaa gctgcaggaa taccgggtct taattaccac cctcatcact
     2941 gccggcaggt tggtctcggc ccagtttccc attgatcact tcacacacat cttcatcgat
     3001 gaggctggcc actgcatgga gcctgagagt ctggtagcta tagcagggct gatggaagta
     3061 aaggaaacag gtgatccagg agggcagctg gtgctggcag gagaccctcg gcagctgggg
     3121 cctgtgctgc gttccccact gacccagaag catggactgg gatactcact gctggagcgg
     3181 ctgctcacct acaactccct gtacaagaag ggccctgatg gctatgaccc ccagttcata
     3241 accaagctgc tccgcaacta caggtctcat cccaccatcc tggacattcc taaccagctc
     3301 tattatgaag gggagctgca ggcctgtgct gatgtcgtgg atcgagaacg cttctgccgc
     3361 tgggcgggcc tacctcgaca gggctttccc atcatctttc acggcgtaat gggcaaagat
     3421 gagcgtgaag gcaacagccc atccttcttc aaccctgaag aggctgccac agtgacttcc
     3481 tacctgaagc tgctcctggc cccctcctcc aagaagggca aagctcgcct gagccctcga
     3541 agtgtgggcg tcatctcccc gtaccggaaa caggtggaga aaatccgtta ctgcatcacc
     3601 aaacttgaca gggagcttcg aggactggat gacatcaagg acttgaaggt gggttcagta
     3661 gaagaattcc aaggccaaga acgaagcgtc atcctcatct ccaccgtgcg aagcagccag
     3721 agctttgtgc agctggatct ggactttaat ctgggtttcc ttaagaaccc caagaggttc
     3781 aatgtagctg tgacccgggc caaggccctg ctcatcatcg tggggaaccc ccttctcctg
     3841 ggccatgacc ctgactggaa agtattcctg gagttctgta aagaaaacgg agggtatacc
     3901 gggtgtccct tccctgccaa actggacctg caacagggac agaatttact gcaaggtctg
     3961 agcaagctca gcccctctac ctcagggccc cacagccatg actacctccc ccaggagcgg
     4021 gagggtgaag ggggcctgtc tctgcaagtg gagccagagt ggaggaatga gctctgagaa
     4081 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
     4141 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4201 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4261 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4321 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4381 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4441 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4501 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4561 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4621 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4681 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4741 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4801 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4861 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4921 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4981 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5041 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5101 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5161 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5221 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5281 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5341 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5401 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5461 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5521 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5581 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5641 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5701 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5761 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5821 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5881 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5941 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6001 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6061 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6121 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6181 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6241 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6361 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6421 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6481 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6541 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6601 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6661 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6721 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6781 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6841 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6901 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6961 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7021 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7081 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7141 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7201 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7261 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7321 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7381 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7441 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7501 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7561 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7621 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7681 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7741 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7801 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7861 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7921 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7981 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8041 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8101 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8161 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8221 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8281 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8341 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8401 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8461 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8521 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8581 acgtc
//
