LOCUS       sequence-354369-       11058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express p65-mRuby2 under p65 promoter (With 
            blasticidin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Lane K, Andres-Terre M, Kudo T, Monack DM, Covert MW
  TITLE     Escalating Threat Levels of Bacterial Infection Can Be Discriminated
            by Distinct MAPK and NF-kappaB Signaling Dynamics in Single Host 
            Cells.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):183-196.e4. doi: 
            10.1016/j.cels.2019.02.008. Epub 2019 Mar 20.
  PUBMED    30904375
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.008"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "183-196.e4"
COMMENT     Sequence Label: pLenti_Blast_DEST mouse_p65promoter_mouse_p65-...
FEATURES             Location/Qualifiers
     source          1..11058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    54..106
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2662..3204
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             3259..3969
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    complement(3996..4020)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4051..4639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4104..4124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4522..4533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4690..4710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4695..5024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4875..5010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5072..5119
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5138..5536
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5623..5856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5928..6049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5965..5984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6019..6038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6089..6224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6244..6263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6245..6263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6273..6290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6273..6289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6282..6304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6431..6886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6518..6537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6728..6749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6912..7016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7017..7085,7086..7877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7235..7254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8048..8636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8537..8556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8790..8807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8924..8945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8960..8965,8966..8983,8984..8990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8998..9014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9003..9025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9022..9038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9022..9038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9057..9077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9059..9077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9105..9331
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9332..9512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9559..9684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10177..10410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10595..10639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10906..11022
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gccggtcgcc
      121 accatgatta tcgactctta actgatgggc catctctcca gcctgcaacc atcctttttt
      181 aaagagcatc ttctgtagtt ctggatgggt tcaaactatg tagaggagga tgacgttgaa
      241 cgtctgaccc catgagcgct gggattactg gagcatcctg gagtcagaga tcaaatccca
      301 gcttcctgca acgttatgca aaaactactg agttctcatt tctagccttt actttttagt
      361 aaggacgctg gtattcatat taacttaaat tgtaactata tgtgttttta caagtggttc
      421 atgtgaggac caatgatggc tgtgtgccat taattggaga ttatattcat tctgtcgcct
      481 tgacatccgc cctcgcaaaa tattaaactg ctttttctaa tctgcgccaa gtgagaaact
      541 gaaatgagtg ggaggggcgt aactattttc acttttgtct tcctgttttt ttcagagcct
      601 gggaaagcaa ttcagaattt ccaatctttt ccaattccca catgatttcc taatttagag
      661 aaaataagtc tttctctgag tgctgaaacc tcctgtggcc cgggaggcgc ctcctccttc
      721 tggcatagtg gaggcagcct gatgtgttgc gtcctccggc cagagtcccc acactcaatc
      781 tgcacacctg cccttggctt agatggcaat ttttgctccc tacaaacgct ttcctcattg
      841 gagcctggac tcctgggagg ccttgaagga gatgttcacg gtgtgaccct agctggttcc
      901 ggagatccct aaagatctaa accttagcag acagcaccta tgtgtgcaga cgaagccctg
      961 gggctcttcc agcactcatg gctctcaggg actcttagag gtacaggtgt ctgcctagtc
     1021 ctcggtctgc ccctctaccc taaggcctgt cggatgcagc acgctcgctc cgcgaagcac
     1081 tcctgcgcgc agccggatct aggttgggga gcatgtggcc cgtagcacgc gggtccccag
     1141 aaggcccctg cacgcttagg aaaacgtgtg gaattcagtc actggcctcc gcgggagcag
     1201 caggcctggg cctctccctg cgcagggcga atggccgcag cctgccacca gccctggctc
     1261 ggctccagtc ccggccaggg tgacatcacc aaactcctga gatcagagcg gggcggggaa
     1321 cgggacccga aatcccctaa aaacagagtg agtaatcgcg ggcccgccct ccaggcgggg
     1381 ccgggaacgg gaccgggacc ccgagcgagc cgcgctgcgg agcttgtagt cgggccggcc
     1441 tcgggacaaa cagcctcggc gtcctcccgc cgcgctgcgg tgtccccgcc cgcgcgcccg
     1501 ccgcacacct tccgcgccca gcgtgagtcg cgcacctgct ctcgtctaga cctgaccatg
     1561 gacgatctgt ttcccctcat ctttccctca gagccagccc aggcttctgg gccttatgtg
     1621 gagatcatcg aacagccgaa gcaacggggc atgcgattcc gctataaatg cgaggggcgc
     1681 tcagcgggca gtattcctgg cgagagaagc acagatacca ccaagacaca ccccaccatc
     1741 aagatcaatg gctacacagg accaggaaca gttcgaatct ccctggtcac caaggatcca
     1801 cctcaccggc ctcatccaca tgaacttgtg gggaaggact gccgggatgg ctactatgag
     1861 gctgacctct gcccagaccg cagtatccat agcttccaga acctggggat ccagtgtgtg
     1921 aagaagcgag acctggagca agccattagc cagcgaatcc agaccaacaa taaccccttt
     1981 cacgttccta tagaggagca gcgcggggac tatgacttga atgcagtgcg cctctgcttc
     2041 caggtgacag tgcgggaccc agcaggcagg cccctcctcc tgacccctgt cctctcacat
     2101 ccgatttttg ataaccgggc ccccaacact gccgagctca agatctgccg agtaaaccgg
     2161 aactctggga gctgcctcgg tggggatgag atcttcttgc tgtgcgacaa ggtgcagaaa
     2221 gaagacattg aggtgtattt cacgggacca ggctgggagg cacgaggctc cttttctcaa
     2281 gctgatgtgc atcggcaagt ggccattgtg ttccggactc ctccgtacgc cgaccccagc
     2341 ctccaggctc ctgttcgagt ctccatgcag ctacggcggc cttctgatcg cgagctcagt
     2401 gagcccatgg agttccagta cttgccagac acagatgatc gccaccggat tgaagagaag
     2461 cgcaaaagga cctatgagac cttcaagagt atcatgaaga agagtccttt caatggacca
     2521 actgaacccc ggcctccaac ccggcgtatt gctgtgccta cccgaaactc aacttctgtc
     2581 cccaagccag ccccgcagcc ctacaccttc ccagcatccc tcagcaccat caactttgat
     2641 gagttttccc ccatgctgtt accatcaggg cagatctcaa accaggccct ggccttagca
     2701 ccgtcctctg ccccagtcct tgcccagacc atggtccctt cctcagccat ggtacctctg
     2761 gctcagcccc cagctcctgc cccagttcta accccgggtc ctccccagtc cctgtctgca
     2821 cctgttccaa agagcaccca ggctggggaa ggcacgctgt cggaagccct gctgcacctg
     2881 cagtttgatg ctgatgaaga cttgggggcc ttgcttggca acagcacaga cccaggagtg
     2941 ttcacagacc tggcatctgt ggacaactca gagtttcagc agctcctgaa ccagggtgtg
     3001 tccatgtctc actccacagc tgagcccatg ctgatggagt accctgaagc tataactcgc
     3061 ctggtgacag ggtcccagag gccccctgac ccagctccca cacccctggg gacctcgggg
     3121 cttcccaatg gtctctccgg agatgaagac ttctcctcca ttgcggacat ggacttctct
     3181 gctcttttga gtcagatcag ctccacccat gggcatggca ccggcagcac cggcagcggc
     3241 agctcatccg gaactagtat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt
     3301 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
     3361 gaaggcaatc cgtacatggg aactcaaacc atgaggatca aagtcatcga gggaggaccc
     3421 ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc
     3481 aagtacccga aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg
     3541 gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt
     3601 gaggatggct gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt
     3661 cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca
     3721 gatggtggtc tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg
     3781 tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc
     3841 ggtatccatg ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc
     3901 gtagtacaac gcgaacacgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag
     3961 ctgtacaagt aagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt
     4021 tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa
     4081 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4141 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4201 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4261 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4321 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4381 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4441 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4501 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4561 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4621 ctttgggccg cctccccgcc tggaattctg cagatatccg gttagtaatg agtttggaat
     4681 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4741 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4801 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4861 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4981 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5041 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     5101 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga
     5161 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     5221 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     5281 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     5341 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     5401 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     5461 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     5521 tgtgtgggag ggctaagcac aattcgagct cggtaccttt aagaccaatg acttacaagg
     5581 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact
     5641 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5701 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5761 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5821 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5881 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6061 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6121 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6181 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6241 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6301 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6421 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8941 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9001 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9061 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     9121 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     9181 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     9241 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     9301 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     9361 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9421 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9481 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9541 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9601 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9661 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9721 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9781 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9841 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9901 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9961 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    10021 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    10081 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10141 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10201 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10261 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    10321 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    10381 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    10441 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    10501 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    10561 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    10621 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    10681 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    10741 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    10801 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    10861 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
    10921 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    10981 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtagt ccagtgtggt
    11041 ggaattctgc agatatca
//
