LOCUS       sequence-354371-       18652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking C, prM, E proteins but with 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 18652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelCME-mVenus
FEATURES             Location/Qualifiers
     source          1..18652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..43
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    61..459
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    719..1192
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1265..2236)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2236..3411)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3737..3987
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3990..4745)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4836..5055
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5123..5654
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5131..5745
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6603..6705
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6706..7365
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6772..6791)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7585..7606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7621..7626,7627..7644,7645..7651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7664..7686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7683..7699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7683..7699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8136..8154
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8136..8153
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(8353..8355,8356..8358,8359..9069)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8398..8419)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8659..8678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9006..9027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     17942..17961
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(18325..18344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18326..18344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18351..18368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18351..18367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18360..18382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(18575..18594)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatcgaata taacttcgta taatgtatgc tatacgaagt tattagcgat gagctcggac
       61 ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagctc
      121 gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
      181 tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
      241 gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
      301 ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
      361 tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaagcaa cttcagacaa
      421 tacaaatcag cgacactgaa tacggggcaa cctcatgtcc gagctcgcga gctcgtcgac
      481 agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag
      541 gtattttgtc cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc
      601 cacagcaggc atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt
      661 caatacttgc ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga
      721 caatacaagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg
      781 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
      841 tcagaccgac gatacgagtg ggaccgtggt tccagactaa taatcagacc gacgatacga
      901 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac catggtccca
      961 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     1021 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     1081 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     1141 atcagaccga cgatacaagt ggaacagtgg gcccagagag aatattcagg ccagttatgc
     1201 tttctggcct gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt
     1261 cgcatcaggg tgctggcttt tcaagttcct taagaatggc ctcaattttc tctatacact
     1321 cagttggaac acgagacctg tccaggttaa gcaccatttt atcgccctta tacaatactg
     1381 tcgctccagg agcaaactga tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt
     1441 taagcacaga agttaaaaga gtgataactt cttcagcttc aaatatcacc ccagcttttt
     1501 tctgctcatg aaggttagat gcctgctgct taagtaattc ctctttatct gtaaaggctt
     1561 tttgaagtgc atcacctgac cgggcagata gttcaccggg gtgagaaaaa agagcaacaa
     1621 ctgatttagg caatttggcg gtgttgatac agcgggtaat aatcttacgt gaaatatttt
     1681 ccgcatcagc cagcgcagaa atatttccag caaattcatt ctgcaatcgg cttgcataac
     1741 gctgaccacg ttcataagca cttgttgggc gataatcgtt acccaatctg gataatgcag
     1801 ccatctgctc atcatccagc tcgccaacca gaacacgata atcactttcg gtaagtgcag
     1861 cagctttacg acggcgactc ccatcggcaa tttctatgac accagatact cttcgaccga
     1921 acgccggtgt ctgttgacca gtcagtagaa aagaagggat gagatcatcc agtgcgtcct
     1981 cagtaagcag ctcctggtca cgttcattac ctgaccatac ccgagaggtc ttctcaacac
     2041 tatcaccccg gagcacttca agagtaaact tcacatcccg accacataca ggcaaagtaa
     2101 tggcattacc gcgagccatt actcctacgc gcgcaattaa cgaatccacc atcggggcag
     2161 ctggtgtcga taacgaagta tcttcaaccg gttgagtatt gagcgtatgt tttggaataa
     2221 caggcgcacg cttcattatc taatctccca gcgtggttta atcagacgat cgaaaatttc
     2281 attgcagaca ggttcccaaa tagaaagagc atttctccag gcaccagttg aagagcgttg
     2341 atcaatggcc tgttcaaaaa cagttctcat ccggatctga cctttaccaa cttcatccgt
     2401 ttcacgtaca acatttttta gaaccatgct tccccaggca tcccgaattt gctcctccat
     2461 ccacggggac tgagagccat tactattgct gtatttggta agcaaaatac gtacatcagg
     2521 ctcgaaccct ttaagatcaa cgttcttgag cagatcacga agcatatcga aaaactgcag
     2581 tgcggaggtg tagtcaaaca actcagcagg cgtgggaaca atcagcacat cagcagcaca
     2641 tacgacatta atcgtgccga tacccaggtt aggcgcgctg tcaataacta tgacatcata
     2701 gtcatgagca acagtttcaa tggccagtcg gagcatcagg tgtggatcgg tgggcagttt
     2761 accttcatca aatttgccca ttaactcagt ttcaatacgg tgcagagcca gacaggaagg
     2821 aataatgtca agccccggcc agcaagtggg ctttattgca taagtgacat cgtccttttc
     2881 cccaagatag aaaggcagga gagtgtcttc tgcatgaata tgaagatctg gtacccatcc
     2941 gtgatacatt gaggctgttc cctgggggtc gttaccttcc acgagcaaaa cacgtagccc
     3001 cttcagagcc agatcctgag caagatgaac agaaactgag gttttgtaaa cgccaccttt
     3061 atgggcagca accccgatca ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc
     3121 aaacacatca cgcatatgat taatttgttc aattgtataa ccaacacgtt gctcaacccg
     3181 tcctcgaatt tccatatccg ggtgcggtag tcgccctgct ttctcggcat ctctgatagc
     3241 ctgagaagaa accccaacta aatccgctgc ttcacctatt ctccagcgcc gggttatttt
     3301 cctcgcttcc gggctgtcat cattaaactg tgcaatggcg atagccttcg tcatttcatg
     3361 accagcgttt atgcactggt taagtgtttc catgagtttc attctgaaca tcctttaatc
     3421 attgctttgc gtttttttat taaatcttgc aatttactgc aaagcaacaa caaaatcgca
     3481 aagtcatcaa aaaaccgcaa agttgtttaa aataagagca acactacaaa aggagataag
     3541 aagagcacat acctcagtca cttattatca ctagcgctcg ccgcagccgt gtaaccgagc
     3601 atagcgagcg aactggcgag gaagcaaaga agaactgttc tgtcagatag ctcttacgct
     3661 cagcgcaaga agaaatatcc accgtgggaa aaactccagg tagaggtaca cacgcggata
     3721 gccaattcag agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc
     3781 cgatatcaag tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc
     3841 aaatttggct tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa
     3901 ggaaacagca aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac
     3961 cctcaaatct gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg
     4021 aaggaaaata cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc
     4081 attggagttc tgctgttgat ctcattaaca cagacctgca ggaagcggcg gcggaagtca
     4141 ggcatacgct ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga
     4201 gagacgatgc ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg
     4261 gcatacggat tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt
     4321 aacccgataa agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat
     4381 ggactgtgcg cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca
     4441 tcctcttcag ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt
     4501 atatccttac tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg
     4561 atctcgcaga taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg
     4621 aacagataca gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg
     4681 tttgactgga cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc
     4741 gccatgacag atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag
     4801 agtcaataag cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca
     4861 gggttaaggg caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa
     4921 agggcaattt gtcacaacac cttctctaga accagcatgg ataaaggcct acaaggcgct
     4981 ctaaaaaaga agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggataa
     5041 gtggataacc ccaagggaag ttttttcagg catcgtgtgt aagcagaata tataagtgct
     5101 gttccctggt gcttcctcgc tcactcgacc gggagggttc gagaaggggg ggcacccccc
     5161 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     5221 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     5281 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     5341 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     5401 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     5461 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     5521 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     5581 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     5641 caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     5701 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagccgag ggcttcgccc
     5761 tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat catggttctg
     5821 tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa cgtaacaccg
     5881 cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg
     5941 catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat taataatgcg
     6001 gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag tggataacag
     6061 ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca tttccatata
     6121 aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca tcagactccg
     6181 gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga
     6241 cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc agcggtatac
     6301 cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg accggaacag
     6361 agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag gtcctgaccg
     6421 ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga cggttacgcc
     6481 gctccatgag cttatcgcga ataaatacct gtgacggaag atcacttcgc agaataaata
     6541 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     6601 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     6661 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     6721 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     6781 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     6841 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     6901 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     6961 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7021 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7081 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7141 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7201 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7261 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     7321 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     7381 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     7441 ggcagaaatt cgatgataag ctgtcaaaca tgagaattgg tcgacggccc gggcggccgc
     7501 aaggagttcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7561 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7621 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7681 cacaggaaac agctatgacc atgattacgc caagctattt aggtgagact atagaatact
     7741 caagcttgca tgcctgcagg tcgactctag aggatcccac attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtgt cgacgcggcc gctagcgatg
     7921 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     7981 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
     8041 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
     8101 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagagtaaat
     8161 cctgtgtgct aattgaggtg cattggtctg caaatcgagt tgctaggcaa taaacacatt
     8221 tggattaatt ttaatcgttc gttgagcgat tagcagagaa ctgaccagaa catgtctggt
     8281 cgtaaagctc agggaaaaac cctgggcgtc aatatggtac gacgaggagt tcgctccttg
     8341 tcaaacgagc tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     8401 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     8461 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     8521 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     8581 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     8641 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     8701 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     8761 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     8821 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     8881 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     8941 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     9001 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     9061 ctgtacaaga ccggtaactt tgaccttctc aagttggccg gcgacgtcga gtccaaccca
     9121 gggcccctgc agcaaatttt cgtgaagacc ctgacgggca agaccatcac tcttgaggtc
     9181 gagcccagtg acaccatcga gaatgtcaag gccaagatcc aagacaagga aggcatccca
     9241 cctgaccagc agaggctgat attcgcgggc aaacagctgg aggatggccg caccctgtcc
     9301 gactacaaca tccagaaaga gtccaccttg cacctggtgc tgtgtctccg cggtggaatg
     9361 tccggtcgta aagcccaggg caaaacccta ggcgtaaata tggtccgacg cggagtccgc
     9421 aacatgacaa tgtccatgag catgatcttg gtaggagtga tcatgatgtt tttgtctcta
     9481 ggagttgggg cggatcaagg atgcgccatc aactttggca agagagagct caagtgcgga
     9541 gatggtatct tcatatttag agactctgat gactggctga acaagtactc atactatcca
     9601 gaagatcctg tgaagcttgc atcaatagtg aaagcctctt ttgaagaagg gaagtgtggc
     9661 ctaaattcag ttgactccct tgagcatgag atgtggagaa gcagggcaga tgagatcaat
     9721 gccatttttg aggaaaacga ggtggacatt tctgttgtcg tgcaggatcc aaagaatgtt
     9781 taccagagag gaactcatcc attttccaga attcgggatg gtctgcagta tggttggaag
     9841 acttggggta agaaccttgt gttctcccca gggaggaaga atggaagctt catcatagat
     9901 ggaaagtcca ggaaagaatg cccgttttca aaccgggtct ggaattcttt ccagatagag
     9961 gagtttggga cgggagtgtt caccacacgc gtgtacatgg acgcagtctt tgaatacacc
    10021 atagactgcg atggatctat cttgggtgca gcggtgaacg gaaaaaagag tgcccatggc
    10081 tctccaacat tttggatggg aagtcatgaa gtaaatggga catggatgat ccacaccttg
    10141 gaggcattag attacaagga gtgtgagtgg ccactgacac atacgattgg aacatcagtt
    10201 gaagagagtg aaatgttcat gccgagatca atcggaggcc cagttagctc tcacaatcat
    10261 atccctggat acaaggttca gacgaacgga ccttggatgc aggtaccact agaagtgaag
    10321 agagaagctt gcccagggac tagcgtgatc attgatggca actgtgatgg acggggaaaa
    10381 tcaaccagat ccaccacgga tagcgggaaa gttattcctg aatggtgttg ccgctcctgc
    10441 acaatgccgc ctgtgagctt ccatggtagt gatgggtgtt ggtatcccat ggaaattagg
    10501 ccaaggaaaa cgcatgaaag ccatctggtg cgctcctggg ttacagctgg agaaatacat
    10561 gctgtccctt ttggtttggt gagcatgatg atagcaatgg aagtggtcct aaggaaaaga
    10621 cagggaccaa agcaaatgtt ggttggagga gtagtgctct tgggagcaat gctggtcggg
    10681 caagtaactc tccttgattt gctgaaactc acagtggctg tgggattgca tttccatgag
    10741 atgaacaatg gaggagacgc catgtatatg gcgttgattg ctgccttttc aatcagacca
    10801 gggctgctca tcggctttgg gctcaggacc ctatggagcc ctcgggaacg ccttgtgctg
    10861 accctaggag cagccatggt ggagattgcc ttgggtggcg tgatgggcgg cctgtggaag
    10921 tatctaaatg cagtttctct ctgcatcctg acaataaatg ctgttgcttc taggaaagca
    10981 tcaaatacca tcttgcccct catggctctg ttgacacctg tcactatggc tgaggtgaga
    11041 cttgccgcaa tgttcttttg tgccatggtt atcatagggg tccttcacca gaatttcaag
    11101 gacacctcca tgcagaagac tatacctctg gtggccctca cactcacatc ttacctgggc
    11161 ttgacacaac cttttttggg cctgtgtgca tttctggcaa cccgcatatt tgggcgaagg
    11221 agtatcccag tgaatgaggc actcgcagca gctggtctag tgggagtgct ggcaggactg
    11281 gcttttcagg agatggagaa cttccttggt ccgattgcag ttggaggact cctgatgatg
    11341 ctggttagcg tggctgggag ggtggatggg ctagagctca agaagcttgg tgaagtttca
    11401 tgggaagagg aggcggagat cagcgggagt tccgcccgct atgatgtggc actcagtgaa
    11461 caaggggagt tcaagctgct ttctgaagag aaagtgccat gggaccaggt tgtgatgacc
    11521 tcgctggcct tggttggggc tgccctccat ccatttgctc ttctgctggt ccttgctggg
    11581 tggctgtttc atgtcagggg agcgcgcaga agtggtcacc ggagaagtgg ggatgtcttg
    11641 tgggatattc ccactcctaa gatcatcgag gaatgtgaac atctggagga tgggatttat
    11701 ggcatattcc agtcaacctt cttgggggcc tcccagcgag gagtgggagt ggcacaggga
    11761 ggggtgttcc acacaatgtg gcatgtcaca agaggagctt tccttgtcag gaatggcaag
    11821 aagttgattc catcttgggc ttcagtaaag gaagaccttg tcgcctatgg tggctcatgg
    11881 aagttggaag gcagatggga tggagaggaa gaggtccagt tgatcgcggc tgttccagga
    11941 aagaacgtgg tcaacgtcca gacaaaaccg agcttgttca aagtgaggaa tgggggagaa
    12001 atcggggctg tcgctcttga ctatccgagt ggcacttcag gatctcctat tgttaacagg
    12061 aacggagagg tgattgggct gtacggcaat ggcatccttg tcggtgacaa ctccttcgtg
    12121 tccgccatat cccagactga ggtgaaggaa gaaggaaagg aggagctcca agagatcccg
    12181 acaatgctaa agaaaggaat gacaactgtc cttgattttc atcctggagc tgggaagaca
    12241 agacgtttcc tcccacagat cttggccgag tgcgcacgga gacgcttgcg cactcttgtg
    12301 ttggccccca ccagggttgt tctttctgaa atgaaggagg cttttcacgg cctggacgtg
    12361 aaattccaca cacaggcttt ttccgctcac ggcagcggga gagaagtcat tgatgccatg
    12421 tgccatgcca ccctaactta caggatgttg gaaccaacta gggttgttaa ctgggaagtg
    12481 atcattatgg atgaagccca ttttttggat ccagctagca tagccgctag aggttgggca
    12541 gcgcacagag ctagggcaaa tgaaagtgca acaatcttga tgacagccac accgcctggg
    12601 actagtgatg aatttccaca ttcaaatggt gaaatagaag atgttcaaac ggacataccc
    12661 agtgagccct ggaacacagg gcatgactgg atcctggctg acaaaaggcc cacggcatgg
    12721 ttccttccat ccatcagagc tgcaaatgtc atggctgcct ctttgcgtaa ggctggaaag
    12781 agtgtggtgg tcctgaacag gaaaaccttt gagagagaat accccacgat aaagcagaag
    12841 aaacctgact ttatattggc cactgacata gctgaaatgg gagccaacct ttgcgtggag
    12901 cgagtgctgg attgcaggac ggcttttaag cctgtgcttg tggatgaagg gaggaaggtg
    12961 gcaataaaag ggccacttcg tatctccgca tcctctgctg ctcaaaggag ggggcgcatt
    13021 gggagaaatc ccaacagaga tggagactca tactactatt ctgagcctac aagtgaaaat
    13081 aatgcccacc acgtctgctg gttggaggcc tcaatgctct tggacaacat ggaggtgagg
    13141 ggtggaatgg tcgccccact ctatggcgtt gaaggaacta aaacaccagt ttcccctggt
    13201 gaaatgagac tgagggatga ccagaggaaa gtcttcagag aactagtgag gaattgtgac
    13261 ctgcccgttt ggctttcgtg gcaagtggcc aaggctggtt tgaagacgaa tgatcgtaag
    13321 tggtgttttg aaggccctga ggaacatgag atcttgaatg acagcggtga aacagtgaag
    13381 tgcagggctc ctggaggagc aaagaagcct ctgcgcccaa ggtggtgtga tgaaagggtg
    13441 tcatctgacc agagtgcgct gtctgaattt attaagtttg ctgaaggtag gaggggagct
    13501 gctgaagtgc tagttgtgct gagtgaactc cctgatttcc tggctaaaaa aggtggagag
    13561 gcaatggata ccatcagtgt gttcctccac tctgaggaag gctctagggc ttaccgcaat
    13621 gcactatcaa tgatgcctga ggcaatgaca atagtcatgc tgtttatact ggctggacta
    13681 ctgacatcgg gaatggtcat ctttttcatg tctcccaaag gcatcagtag aatgtctatg
    13741 gcgatgggca caatggccgg ctgtggatat ctcatgttcc ttggaggcgt caaacccact
    13801 cacatctcct atgtcatgct catattcttt gtcctgatgg tggttgtgat ccccgagcca
    13861 gggcaacaaa ggtccatcca agacaaccaa gtggcatacc tcattattgg catcctgacg
    13921 ctggtttcag cggtggcagc caacgagcta ggcatgctgg agaaaaccaa agaggacctc
    13981 tttgggaaga agaacttaat tccatctagt gcttcaccct ggagttggcc ggatcttgac
    14041 ctgaagccag gagctgcctg gacagtgtac gttggcattg ttacaatgct ctctccaatg
    14101 ttgcaccact ggatcaaagt cgaatatggc aacctgtctc tgtctggaat agcccagtca
    14161 gcctcagtcc tttctttcat ggacaagggg ataccattca tgaagatgaa tatctcggtc
    14221 ataatgctgc tggtcagtgg ctggaattca ataacagtga tgcctctgct ctgtggcata
    14281 gggtgcgcca tgctccactg gtctctcatt ttacctggaa tcaaagcgca gcagtcaaag
    14341 cttgcacaga gaagggtgtt ccatggcgtt gccaagaacc ctgtggttga tgggaatcca
    14401 acagttgaca ttgaggaagc tcctgaaatg cctgcccttt atgagaagaa actggctcta
    14461 tatctccttc ttgctctcag cctagcttct gttgccatgt gcagaacgcc cttttcattg
    14521 gctgaaggca ttgtcctagc atcagctgcc ttagggccgc tcatagaggg aaacaccagc
    14581 cttctttgga atggacccat ggctgtctcc atgacaggag tcatgagggg gaatcactat
    14641 gcttttgtgg gagtcatgta caatctatgg aagatgaaaa ctggacgccg ggggagcgcg
    14701 aatggaaaaa ctttgggtga agtctggaag agggaactga atctgttgga caagcgacag
    14761 tttgagttgt ataaaaggac cgacattgtg gaggtggatc gtgatacggc acgcaggcat
    14821 ttggccgaag ggaaggtgga caccggggtg gcggtctcca gggggaccgc aaagttaagg
    14881 tggttccatg agcgtggcta tgtcaagctg gaaggtaggg tgattgacct ggggtgtggc
    14941 cgcggaggct ggtgttacta cgctgctgcg caaaaggaag tgagtggggt caaaggattt
    15001 actcttggaa gagacggcca tgagaaaccc atgaatgtgc aaagtctggg atggaacatc
    15061 atcaccttca aggacaaaac tgatatccac cgcctagaac cagtgaaatg tgacaccctt
    15121 ttgtgtgaca ttggagagtc atcatcgtca tcggtcacag agggggaaag gaccgtgaga
    15181 gttcttgata ctgtagaaaa atggctggct tgtggggttg acaacttctg tgtgaaggtg
    15241 ttagctccat acatgccaga tgttcttgag aaactggaat tgctccaaag gaggtttggc
    15301 ggaacagtga tcaggaaccc tctctccagg aattccactc atgaaatgta ctacgtgtct
    15361 ggagcccgca gcaatgtcac atttactgtg aaccaaacat cccgcctcct gatgaggaga
    15421 atgaggcgtc caactggaaa agtgaccctg gaggctgacg tcatcctccc aattgggaca
    15481 cgcagtgttg agacagacaa gggacccctg gacaaagagg ccatagaaga aagggttgag
    15541 aggataaaat ctgagtacat gacctcttgg ttttatgaca atgacaaccc ctacaggacc
    15601 tggcactact gtggctccta tgtcacaaaa acctcaggaa gtgcggcgag catggtaaat
    15661 ggtgttatta aaattctgac atatccatgg gacaggatag aggaggtcac aagaatggca
    15721 atgactgaca caaccccttt tggacagcaa agagtgttta aagaaaaagt tgacaccaga
    15781 gcaaaggatc caccagcggg aactaggaag atcatgaaag ttgtcaacag gtggctgttc
    15841 cgccacctgg ccagagaaaa gaaccccaga ctgtgcacaa aggaagaatt tattgcaaaa
    15901 gtccgaagtc atgcagccat tggagcttac ctggaagaac aagaacagtg gaagactgcc
    15961 aatgaggctg tccaagaccc aaagttctgg gaactggtgg atgaagaaag gaagctgcac
    16021 caacaaggca ggtgtcggac ttgtgtgtac aacatgatgg ggaaaagaga gaagaagctg
    16081 tcagagtttg ggaaagcaaa gggaagccgt gccatatggt atatgtggct gggagcgcgg
    16141 tatcttgagt ttgaggccct gggattcctg aatgaggacc attgggcttc cagggaaaac
    16201 tcaggaggag gagtggaagg cattggctta caatacctag gatatgtgat cagagacctg
    16261 gctgcaatgg atggtggtgg attctacgcg gatgacaccg ctggatggga cacgcgcatc
    16321 acagaggcag accttgatga tgaacaggag atcttgaact acatgagccc acatcacaaa
    16381 aaactggcac aagcagtgat ggaaatgaca tacaagaaca aagtggtgaa agtgttgaga
    16441 ccagccccag gagggaaagc ctacatggat gtcataagtc gacgagacca gagaggatcc
    16501 gggcaggtag tgacttatgc tctgaacacc atcaccaact tgaaagtcca attgatcaga
    16561 atggcagaag cagagatggt gatacatcac caacatgttc aagattgtga tgaatcagtt
    16621 ctgaccaggc tggaggcatg gctcactgag cacggatgtg acagactgaa gaggatggcg
    16681 gtgagtggag acgactgtgt ggtccggccc atcgatgaca ggttcggcct ggccctgtcc
    16741 catctcaacg ccatgtccaa ggttagaaag gacatatctg aatggcagcc atcaaaaggg
    16801 tggaatgatt gggagaatgt gcccttctgt tcccaccact tccatgaact acagctgaag
    16861 gatggcagga ggattgtggt gccttgccga gaacaggacg agctcattgg gagaggaagg
    16921 gtgtctccag gaaacggctg gatgatcaag gaaacagctt gcctcagcaa agcctatgcc
    16981 aacatgtggt cactgatgta ttttcacaaa agggacatga ggctactgtc attggctgtt
    17041 tcctcagctg ttcccacctc atgggttcca caaggacgca caacatggtc gattcatggg
    17101 aaaggggagt ggatgaccac ggaagacatg cttgaggtgt ggaacagagt atggataacc
    17161 aacaacccac acatgcagga caagacaatg gtgaaaaaat ggagagatgt cccttatcta
    17221 accaagagac aagacaagct gtgcggatca ctgattggaa tgaccaatag ggccacctgg
    17281 gcctcccaca tccatttagt catccatcgt atccgaacgc tgattggaca ggagaaatac
    17341 actgactacc taacagtcat ggacaggtat tctgtggatg ctgacctgca actgggtgag
    17401 cttatctgaa acaccatcta acaggaataa ccgggataca aaccacgggt ggagaaccgg
    17461 actccccaca acctgaaacc gggatataaa ccacggctgg agaaccggac tccgcactta
    17521 aaatgaaaca gaaaccggga taaaaactac ggatggagaa ccggactcca cacattgaga
    17581 cagaagaagt tgtcagccca gaaccccaca cgagttttgc cactgctaag ctgtgaggca
    17641 gtgcaggctg ggacagccga cctccaggtt gcgaaaaacc tggtttctgg gacctcccac
    17701 cccagagtaa aaagaacgga gcctccgcta ccaccctccc acgtggtggt agaaagacgg
    17761 ggtctagagg ttagaggaga ccctccaggg aacaaatagt gggaccatat tgacgccagg
    17821 gaaagaccgg agtggttctc tgcttttcct ccagaggtct gtgagcacag tttgctcaag
    17881 aataagcaga cctttggatg acaaacacaa aaccactcga gcaagacgtt tcccgttgaa
    17941 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
    18001 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
    18061 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
    18121 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
    18181 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
    18241 atgagtcagc aacaccttct tcacgaggca gacctcagcg ctagcggagt gtagtgggat
    18301 ccccgggtac cgagctcgaa ttcgccctat agtgagtcgt attacaattc actggccgtc
    18361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    18421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    18481 cagttgcgca gctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
    18541 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    18601 taagccagcc ccgacacccg ccaacacccg ctgacgcgaa ccccttgcgg cc
//
