LOCUS       sequence-354407-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(194..1384)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1013..1032
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1432..1437,1438..1454,1455..1460))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1587..2175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1667..1686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2584..2600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2584..2600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taatacgact cactataggg gatattttat tttctcccaa tcaggcttga tccccagtaa
       61 gtcaaaaaat agctcgacat actgttcttc cccgatcaag ggttggtttg cgcattcaca
      121 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
      181 cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga
      241 ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg
      301 aggcggcata aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag
      361 ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
      421 cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
      481 tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg
      541 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
      601 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
      661 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
      721 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
      781 agctgactgg gttgaaggct ctcaagggca tgggtcggcg ctctccctta tgcgactcct
      841 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
      901 gtgcatgtaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
      961 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     1021 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     1081 cggcgtagag aatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc
     1141 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa
     1201 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg
     1261 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc
     1321 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
     1381 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct
     1441 aatcgatgat aagctgtcaa acatgagaat taattcatgt tctttcctgc gttatcccct
     1501 gattctgtgg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2221 gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgaccagat atggtctcaa
     2281 ccacttgatc tggggtgagt ccgtgggctt gacagagaac tggaaggagg cgctgaaccg
     2341 tctcaagagc ctgtttgcca ccaatattac tagcaatcgc tacaacttgc gctggggtta
     2401 gtccgtgggc ttgacagaga actggtagaa ggcgttggac ggtttcaaga gcctgtttgc
     2461 caccgtcatg actagcaatc gctacaacct gcgagaccag ctttggtcta gaggaggcat
     2521 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
     2581 tgccaggaaa cagctatgac catg
//
