LOCUS       sequence-354411-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     357..373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     357..373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     378..397
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        378..396
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(571..1761)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1390..1409
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1809..1814,1815..1831,1832..1837))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2044..2063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taactcacgt taagggattt tggtcatggg tggctcgacc agatatggtc tcattgctac
       61 aacttgatct ggggtgagtc cgtgggcttg acagagaact ggaaggaggc gctgaaccgt
      121 ctcaagagcc tgtttgccac caatattact agcaatcgct acaacttgcg ctggggttag
      181 tccgtgggct tgacagagaa ctggtagaag gcgttggacg gtttcaagag cctgtttgcc
      241 accgtcatga ctagcaatcg ctaccgagac cagctttggt ctagaggagg catgatgata
      301 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
      361 aaacagctat gaccatgtaa tacgactcac tataggggat attttatttt ctcccaatca
      421 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatcaagggt
      481 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
      541 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
      601 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
      661 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga
      721 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
      781 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
      841 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
      901 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
      961 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     1021 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     1081 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     1141 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc
     1201 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     1261 cgccgccgca aggaatggtg catgtaagga gatggcgccc aacagtcccc cggccacggg
     1321 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1381 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1441 gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc
     1501 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
     1561 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
     1621 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
     1681 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     1741 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     1801 actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct
     1861 ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgac gcg
//
