LOCUS       sequence-35442-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, 
            Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li 
            X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T,
            Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, 
            Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, 
            Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu
            B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L,
            Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar
            Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley
            DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu 
            VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, 
            Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, 
            Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, 
            Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, 
            Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D
  TITLE     Community-Wide Assessment of Protein-Interface Modeling Suggests 
            Improvements to Design Methodology.
  JOURNAL   J Mol Biol. 2011 Sep 29.
  PUBMED    22001016
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Sep 29."
COMMENT     Sequence Label: pETCON-HB44/design_80
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             308..319
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             320..346
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             431..451
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctactt catacatttt caattaagat gcagttactt cgctgttttt caatattttc
       61 tgttattgct tcagttttag cacaggaact gacaactata tgcgagcaaa tcccctcacc
      121 aactttagaa tcgacgccgt actctttgtc aacgactact attttggcca acgggaaggc
      181 aatgcaagga gtttttgaat attacaaatc agtaacgttt gtcagtaatt gcggttctca
      241 cccctcaaca actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag
      301 ctcgacgatt gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg
      361 tggaggaggc tctggtggag gcggtagcgg aggcggaggg tcggctagcc atatggtaga
      421 tttgggtaca gaaaacttat acttccaatc catggtccaa agagttgaaa tccataagtt
      481 gagacaaggt gaaaacttga tcttgggttt ttcaatcggt ggtggttggt tacaagaccc
      541 atcccaaaat cctttcagtg aagataagac tgacattggt atatacgtaa caagagtctc
      601 tgaaggtggt ccagctgaaa tagcaggttt gcaaatcggt gacaagataa tgcaagttaa
      661 cggtaaaaga atggacaacg tattccacaa cgaagccaga aatttgttaa cccaaagatc
      721 tgaagaagtt gtaagattgt tagtcactag acaatcattg caaaaagctg ttcaacaatc
      781 tatgttatca gaaaccgaag ttctcgaggg gg
//
